Study of corpus callosum in experimental hydrocephalic wistar rats

Lopes, Luíza da Silva; Machado, Hélio Rubens; Lachat, João José

doi:10.1590/s0102-86502003001200005

Cited by 12 publications

(4 citation statements)

References 26 publications

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“…This suggests an attempt of to remyelination. These data are in agreement with the work of Del Bigio 18 and Lopes 19 . Further studies are needed to better understand the pathophysiology of hydrocephalus lesions produced in the corpus callosum and other structures of the brain parenchyma.…”

Section: Discussionsupporting

confidence: 93%

Changes caused by hidrocephalus, induced by kaolin, in the corpus callosum of adult dogs

Cardoso¹,

Lachat²,

Lopes³

et al. 2011

Acta Cir. Bras.

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PURPOSE:To analyze the ventricular enlargement and myelination of the corpus callosum in adult dogs after four and eight weeks of kaolin-induction of hydrocephalus. METHODS: 36 dogs were randomly divided into 3 groups: 1 -without hydrocephalus, 2 -kaolin-induction of hydrocephalus until the fourth week, and 3 -kaolin-induction of hydrocephalus until the eighth week. Ventricular ratios and volumes were calculated using magnetic resonance images, and myelination of the corpus callosum were histologically evaluated using solocromo-cianin stain. RESULTS: Radiological hydrocephalus was observed in 93.75% and overall mortality was 38.4%. Ventricular volumes and ratios were higher in groups 2 and 3 compared to group 1 and similar when measures in the fourth and eighth weeks were compared in the group 3. Indices of luminescence in the knee and in the splenium of the corpus callosum were higher in group 2 than in group 1 indicating that there was loss of myelin in group 2, and similar in groups 1 and 3, showing a tendency to remyelination after 8 weeks. CONCLUSION: The corpus callosum of dogs with kaolin-induced hydrocephalus responds with demyelination of the knee and splenium by the fourth week with a tendency to remyelination by the eighth week. Key words: Hydrocephalus. Corpus Callosum. Myelin Sheath. Dogs. RESUMO OBJETIVO:Analisar a dilatação ventricular e a mielinização do corpo caloso em cães adultos após quatro e oito semanas da indução de hidrocefalia por caulin. MÉTODOS: 36 cães foram aleatoriamente divididos em 3 grupos: 1-sem hidrocefalia, 2-quatro semanas de hidrocefalia induzida por caulin, 3-oito semanas de hidrocefalia induzida por caulin. As razões e volumes ventriculares foram calculados utilizando imagens de ressonância magnética, e, a mielinização do corpo caloso por estudo histológico (coloração com solocromo-cianina). RESULTADOS: Hidrocefalia foi observada radiologicamente em 93,75% e a mortalidade global foi de 38,4%. Os volumes e as razões ventriculares foram maiores nos grupos 2 e 3 em relação ao grupo 1 e semelhantes nas quarta e oitava semanas no grupo 3. Índices de luminescência no joelho e no esplênio do corpo caloso foram maiores no grupo 2 em relação ao grupo 1, indicando que houve perda de mielina no grupo 2, e semelhantes nos grupos 1 e 3, mostrando uma tendência à remielinização em torno de 8 semanas. CONCLUSÃO: O corpo caloso de cães com hidrocefalia induzida por caulin responde com desmielinização no joelho e esplênio em torno de quatro semanas com tendência à remielinização em torno da oitava semana. Descritores: Hidrocefalia. Corpo Caloso. Bainha de Mielina. Cães. Changes caused by hidrocephalus, induced by kaolin, in the corpus callosum of adult dogsActa Cirúrgica Brasileira -Vol. 26 (Suppl. 2) 2011 -9

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Section: Discussionsupporting

confidence: 93%

Changes caused by hidrocephalus, induced by kaolin, in the corpus callosum of adult dogs

Cardoso¹,

Lachat²,

Lopes³

et al. 2011

Acta Cir. Bras.

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“…They lost weight in the first week after induction of hydrocephalus and gained weight in the second week (at par with the age-matched controls) but lost weight again in the third week. The weight loss in the first week may be due to a reduction in breastfeeding activity in the early phase of induction of hydrocephalus, while the loss in the third week may be due to adaptation of the animals with the new condition and before return to normal feeding [41]. The reduction in weight observed in the hydrocephalic groups may also be due to the apathy and loss of appetite [30].…”

Section: Discussionmentioning

confidence: 99%

Changes in Neuronal Density of the Sensorimotor Cortex and Neurodevelopmental Behaviour in Neonatal Mice with Kaolin-Induced Hydrocephalus

Femi-Akinlosotu

Shokunbi

2020

Pediatr Neurosurg

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Introduction: Hydrocephalus is a disorder in which the circulation of cerebrospinal fluid is altered in a manner that leads to its accumulation in the ventricles and subarachnoid space. Its impact on the neuronal density and networks in the overlying cerebral cortex in a time-dependent neonatal hydrocephalic process is largely unknown. We hypothesize that hydrocephalus will affect the cytoarchitecture of the cerebral cortical mantle of neonatal hydrocephalic mice, which will in turn modify sensorimotor processing and neurobehaviour. Objective: The purpose of this study is to probe the effect of hydrocephalus on 3 developmental milestones (surface righting reflex, cliff avoidance reflex, and negative geotaxis) and on cortical neuronal densities in neonatal hydrocephalic mice. Methods: Hydrocephalus was induced in 1-day-old mice by intracisternal injection of sterile kaolin suspension. The pups were tested for reflex development and sensorimotor ability using surface righting reflex (PND 5, 7, and 9), cliff avoidance (PND 6), and negative geotaxis (PND 10 and 12) prior to their sacrifice on PND 7, 14, and 21. Neuronal density and cortical thickness in the sensorimotor cortex were evaluated using atlas-based segmentation of the neocortex and boundary definition in 4-μm paraffin-embedded histological sections with hematoxylin and eosin as well as cresyl violet stains. Results: Surface righting and cliff avoidance activities were significantly impaired in hydrocephalic pups but no statistically significant difference was observed in negative geotaxis in both experimental and control pups. The neuronal density of the sensorimotor cortex was significantly higher in hydrocephalic mice than in age-matched controls on PND 14 and 21 (373.20 ± 21.54 × 10−6 μm2 vs. 157.70 ± 21.88 × 10−6 μm2; 230.0 ± 44.1 × 10−6 μm2 vs. 129.60 ± 3.72 × 10−6 μm2, respectively; p < 0.05). This was accompanied by reduction in the cortical thickness (µm) in the hydrocephalic mice on PND 7 (2,409 ± 43.37 vs. 3,752 ± 65.74, p < 0.05), PND 14 (2,035 ± 322.10 vs. 4,273 ± 67.26, p < 0.05), and PND 21 (1,676 ± 33.90 vs. 4,945 ± 81.79, p < 0.05) compared to controls. Conclusion: In this murine model of neonatal hydrocephalus, the quantitative changes in the cortical neuronal population may play a role in the observed changes in neurobehavioural findings.

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“…The same was found in the study by Khan et al [3], who investigated brain damage in kaolin-induced hydrocephalus in young rats. By studying the corpus callosum in experimental hydrocephalus, Lopes et al [16] observed that the ependyma appeared to be lesioned at an early stage, firstly in the subsequent region of the lateral ventricles, progressing to their anterior portions. Next, ependymal breakage points were observed, and they developed to the almost total destruction of the epithelium, thus undressing the ventricular roof and only leaving islands of ependymal cells.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Morphometric Analysis of the Hippocampus in Wistar Rats with Experimental Hydrocephalus

Taveira

Carraro²,

Catalão³

et al. 2012

Pediatr Neurosurg

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Background: The present study aims at better establishing the alterations caused by the usual enlargement of brain ventricles in this structure. Methods: Hydrocephalus was induced in 7-day-old Wistar rats by the injection of kaolin into the cisterna magna. Morphological studies were performed on the hippocampus 7, 14 and 21 days after injection. The total number of neurons in each hippocampus subarea as well as that of pyknotic neurons were counted. Then we calculated the pyknotic index (PI) by hippocampal subarea, taking into account the level of ventricular dilatation and time of induction of hydrocephalus. Results: PI was statistically larger in the CA1 subarea of the experimental group after 1 week of hydrocephalus induction as compared to the corresponding control as well as in animals that had developed mild hydrocephalus in groups G1, G2 and G3. Conclusion: Hydrocephalus caused morphological alterations in the hippocampus, leading to important changes in its shape.

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Study of corpus callosum in experimental hydrocephalic wistar rats

Cited by 12 publications

References 26 publications

Changes caused by hidrocephalus, induced by kaolin, in the corpus callosum of adult dogs

Changes caused by hidrocephalus, induced by kaolin, in the corpus callosum of adult dogs

Changes in Neuronal Density of the Sensorimotor Cortex and Neurodevelopmental Behaviour in Neonatal Mice with Kaolin-Induced Hydrocephalus

Morphological and Morphometric Analysis of the Hippocampus in Wistar Rats with Experimental Hydrocephalus

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