Morphometric study of gastric mucosa in dogs submitted to proximal gastric vagotomy, splenectomy or proximal gastric vagotomy associated with splenectomy

Abstract

OBJECTIVE: The aim of the present study was to evaluate the effects of total splenectomy and proximal gastric vagotomy (PGV) isolated or associated to the vascularization of the stomach. METHODS: Twenty-eight dogs were distributed in: group A - animals undergoing laparotomy and gastric manipulation; group B - animals undergoing PGV; group C - animals undergoing PGV and splenectomy; and group D - animals undergoing splenectomy alone. All animals were sacrificed on the 7th postoperative day, and immediately followed by infusion of xanthene dye into the thoracic aorta. On the gastric mucous surface, the formation of stained areas and other areas lacking staining was measured by millimeter squares, planimetry and the computerized morphometry method. RESULTS: The formation of an area lacking in staining along the lesser gastric curvature was seen in all animals of groups B and C, which was significantly increased in group C, although in groups A and D such areas were not observed. CONCLUSION: These results showed that, in animals with splenectomy, the vascularization of the stomach was capable of perfusing the whole organ surface, but in animals with PGV alone or associated with splenectomy there was a notable decrease in blood supply throughout the area of the lesser gastric curvature, which suggests the ischemic impairment of this region.

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

“…Variables that can be quantitatively evaluated, such as colon crypt length, thicknesses of the different layers of the intestinal wall and number of caliciform cells can be determined objectively, thereby enabling conclusions that are more precise. Computer-assisted analysis has been used with various objectives and, in relation to conventional methods, it has the advantage of enabling fast and objective quantitative evaluation of the microscopic structures 22,23 . Few studies have evaluated inflammatory alterations in the colon wall of intestinal segments that have been taken out of function, by means of morphometric analysis methods 9,11,15 .…”

Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

“…With the advent of computer-assisted image analysis software, it has become possible to objectively quantify the tissue expression of various proteins, stained using histochemical or immunohistochemical techniques 16,17 . With this method, it has become possible to quantify the expression of sulfomucins and sialomucins in the colon mucosa more precisely, thereby observing changes in total acid mucin content and the influence of the duration of exclusion, by comparing segments with and without fecal stream.…”

Tissue quantification of neutral and acid mucins in the mucosa of the colon with and without fecal stream in rats

“…However, the neutral mucins was not evaluated and the quantification of the acids mucins was conducted subjectively and its concentration in the colon epithelium was not quantified using methodology of greater precision. With the advent of computer-assisted image analysis software, it has become possible to precisely determine macroscopic and microscopic changes such as number of goblet cells and the tissue expression of various substances 11,12,13 . With this technology, the neutral and acids mucins expression in the colon mucosa can be quantified, and the possibility of differences in total content between segments with and without fecal stream can be investigated, along with the influence of the duration of intestinal exclusion on the intensity of expression of these glycoprotein's.…”