A new method for the experimental induction of secundary biliary cirrhosis in wistar rats

Abstract

The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.

Comparative study between ligature with thread or metallic clamping by means of laparoscopy with the purpose of experimental biliary obstruction in swines

“…But, seven days after the procedure for biliary obstruction, the animals showed strongly yellow urine, skin and mucous membranes icteric, ranging from mild to severe; stools lighter shade than that observed before ligation, so as to hepatomegaly palpation. Signs compatibles with what is described on cholestasis in humans 1,48,49 , dogs 9,18 , mices 20 and rats 22,23 . However, in less time than that observed in other animal species researchs 20,22 .…”

“…Signs compatibles with what is described on cholestasis in humans 1,48,49 , dogs 9,18 , mices 20 and rats 22,23 . However, in less time than that observed in other animal species researchs 20,22 . The development of jaundice justifies the color changes of skin and mucous membranes observed, because bilirubin is deposited in tissues such as in high concentrations 49,50 .…”

“…However, in recent years, there was an increase in the diagnosis of asymptomatic lithiasis due to upgrading diagnostic methods routinely performed in patients [14][15][16][17] . Because of its frequency, many researchs are conducted to expand the knowledge about the pathogenesis and improving treatment of this disease 14,15,[18][19][20][21][22][23][24] . The treatment of biliary obstructions depends on the time between occurrence and diagnosis of the injury and, the type, extent and location of same in the bile ducts 14,25 , being the surgical treatment the most recommended 2,26 .…”

Liver cirrhosis on the colonic anastomotic healing in rats

“…Jaundice, when not accompanied by hypoproteinemia, does not increase the rate of dehiscence, probably because hepatic function is not significantly impaired before hypoproteinemia is established. Increased rates of abdominal wall dehiscence and incisional hernias have been found in jaundiced patients 20 .…”

“…Other authors have published studies evaluating the healing of peptic ulcers in patients with hepatic cirrhosis 20 , but there are few studies on hepatic cirrhosis and anastomosis healing [21][22][23] . Patients with cirrhosis may require colonic anastomosis, which causes concern on the part of the surgeon 22 .…”