Método para o estudo in vivo da angiogênese: indução de neovascularização na córnea de coelho

González, Raimundo Pajón; Leyva, A.; Melo, Ramon Andrade Bezerra; Moreira, Rafael Dias Marques; Pessoa, Cláudia; Farias, Romulo Feio; Moraes, Manoel Odoríco de

doi:10.1590/s0102-86502000000300006

Cited by 11 publications

(15 citation statements)

References 9 publications

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“…In the heart in particular, angiogenesis promotes vascular ramification of coronary vessels, increase in blood flow, and cardiac contractility. Angiogenesis also occurs in pathological processes such as chronic arthropathy, psoriasis, diabetic retinopathy, tumoral growth, and metastasis dissemination (González et al, 2000;Safatle et al, 2002;Folkman, 1976Folkman, , 2004.…”

Section: Introductionmentioning

confidence: 99%

Angiogenic activity of latex from Euphorbia tirucalliLinnaeus 1753 (Plantae, Euphorbiaceae)

et al. 2015

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To assess the pro-angiogenic activity of Euphorbia tirucalli, commonly known as "avelós" plant, we performed a series of tests by applying an aqueous E. tirucalli latex solution (10 mg/mL) to the chorioallantoic membranes (CAMs) of 80 fertilized chicken eggs incubated in a temperature-and humidity-controlled automatic incubator. The results indicated that the aqueous latex solution increased vascular network formation compared to that with the negative control (p < 0.05) and the inhibitor control (p < 0.05). This suggests that under the experimental conditions tested, the aqueous latex solution induced an inflammatory response leading to neoangiogenesis.Keywords: angiogenesis, chorioallantoic membrane, Euphorbia tirucalli. Atividade angiogênica do látex da Euphorbia tirucalliLinnaeus 1753 (Plantae, Euphorbiaceae) ResumoCom o objetivo de analisar a atividade angiogênica apresentada pela Euphorbia tirucalli, popularmente conhecida comumente como "avelós", foram realizados ensaios utilizando solução aquosa de látex na concentração de 10 mg/ml, aplicada em membrana corioalantóide (MCA) de 80 ovos férteis de galinha, incubado em estufa automática com temperatura e umidade controladas. Os resultados apontaram que a ação da solução aquosa provocou aumento da percentagem da rede vascular formada em relação aos controles negativo (p<0,05) e inibidor (p<0,05), indicando que nas condições deste experimento, foi responsável pela ativação da resposta inflamatória e crescimento de novos vasos sanguíneos.Palavras-chave: angiogênese, membrana corioalantóide, Euphorbia tirucalli.

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Section: Introductionmentioning

confidence: 99%

Angiogenic activity of latex from Euphorbia tirucalliLinnaeus 1753 (Plantae, Euphorbiaceae)

et al. 2015

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“…The implantation of pellets containing drugs or pro-angiogenic molecules are emerging in the literature as reliable strategies. However, the vascular response associated with these procedures is very intense and unpredictable 25 . In addition, the investment required for pellet production is high.…”

Section: ■ Discussionmentioning

confidence: 99%

Corneal angiogenesis based on different protocols of alkaline cauterization in murine models

et al. 2017

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Purpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer's lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.

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“…Many methods have been developed to study angiogenesis because, when known how to control it, there is a great probability to prevent many of the major causes of visual loss (12)(13) . The technique of corneal vascularization induction was developed by Gimbrone Junior et al (1974) (14) .…”

Section: Discussionmentioning

confidence: 99%

Clinical evaluation and induced corneal vascularization study by native and anionic collagen membranes in rabbits corneas

Binotto¹,

Andrade²,

Costa³

et al. 2009

Arq. Bras. Oftalmol.

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Método para o estudo in vivo da angiogênese: indução de neovascularização na córnea de coelho

Cited by 11 publications

References 9 publications

Angiogenic activity of latex from Euphorbia tirucalliLinnaeus 1753 (Plantae, Euphorbiaceae)

Angiogenic activity of latex from Euphorbia tirucalliLinnaeus 1753 (Plantae, Euphorbiaceae)

Corneal angiogenesis based on different protocols of alkaline cauterization in murine models

Clinical evaluation and induced corneal vascularization study by native and anionic collagen membranes in rabbits corneas

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