Avaliação microscópica e ultra-estrutural do endotélio de veia safena preparada pela técnica "no touch"

Silva, Virgílio Figueiredo; Ishigai, Márcia Marcelino de Souza; Freymüller, Edna; Branco, João Nelson Rodrigues; Gaia, Diego Felipe; Gabriel, Edmo Atique; Romão, Renata Aparecida Leonel; Buffolo, Ênio

doi:10.1590/s0102-76382008000300007

Cited by 7 publications

(3 citation statements)

References 16 publications

(15 reference statements)

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“…This technique stains the extracellular matrix blue and the cellular portion red 10. Using Masson's trichrome staining, the saphenous veins that were endoscopically harvested with a no-touch technique showed well-preserved collagen fibers, whereas those harvested using conventional techniques showed more subendothelial collagen degradation 11. In comparison, the architectures of the radial arterial grafts were preserved with both endoscopic and conventional techniques,8 indicating that the wall structures of the vein grafts were more prone to being damaged by surgical maneuvers.…”

Section: Discussionmentioning

confidence: 99%

Transforming growth factor-β in graft vessels: histology and immunohistochemistry

Yuan

Wang

Shen

et al. 2011

Clinics

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OBJECTIVES:The biological functions of transforming growth factor-β signaling that involves Smad proteins have not been previously investigated with respect to coronary artery bypass grafts. The aim of the present study was to observe the immunostaining of proteins that are related to this signaling pathway.METHODS:Fifteen remnants of coronary artery bypass grafts, including nine saphenous veins, three radial arteries and three mammary arteries, were collected from 12 patients who were undergoing coronary artery bypass. Hematoxylin and eosin, Masson's trichrome, and immunohistochemical staining of transforming growth factor-β1, type I receptor of transforming growth factor-β, Smad2/3, Smad4, and Smad7 were performed.RESULTS:The saphenous veins showed more severe intimal degeneration, more severe smooth muscle cell proliferation and more collagen deposition than the arterial grafts, as evidenced by hematoxylin and eosin and Masson's trichrome stainings. Immunohistochemical assays demonstrated that the majority of the transforming growth factor -β1 signaling cytokines were primarily localized in the cytoplasm in the medial layers of all three types of grafts, whereas ectopic transforming growth factor-β1, type I receptor of transforming growth factor-β, and Smad7 overexpressions in the interstices were observed particularly in the saphenous vein and radial arterial grafts.CONCLUSION:Enhanced transforming growth factor-β1 signal transduction with medial smooth muscle cell proliferation and ectopic transforming growth factor-β1, the presence of the type I receptor of transforming growth factor-β, and Smad7 overexpressions in the extracellular matrix may provide primary evidence for early or late graft failure.

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Section: Discussionmentioning

confidence: 99%

Transforming growth factor-β in graft vessels: histology and immunohistochemistry

Yuan

Wang

Shen

et al. 2011

Clinics

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“…Other important studies were those on the best preservation of venous grafts 34,35 , the use of arterial grafts 36,37 and the comparison between the on-or off-pump CABG surgery 38 .…”

Section: Introductionmentioning

confidence: 99%

Impacto das publicações científicas brasileiras nos recentes avanços científicos na cardiologia

Bacal¹

2009

Arq. Bras. Cardiol.

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The Brazilian research, in the clinical and surgical areas as well as in the basic areas, has developed greatly in recent years. Cardiology, in turn, has effectively contributed for this development. Currently, the Brazilian Archives of Cardiology (Arquivos Brasileiros de Cardiologia), an ISI-indexed journal, joins other important national and also indexed journals, offering the national and international researchers good options for the publication of their studies. This Editorial will discuss the impact of the recent Brazilian scientific publications from the clinical and surgical areas in Cardiology.

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“…When performing OVH, the recent 2014 ESC/EACTS Guidelines on myocardial revascularization support the use of the notouch technique to reduce graft injury and improve patency [ 6 ] . There is evidence that the improved patency of no-touch SV over conventional OVH grafts is associated with a reduction in vascular damage [ 15 ] , maintaining normal vessel architecture, preservation of an intact endothelium [ 16 , 17 ] and endotheliumderived NO [ 17 ] , as well as preservation of the vasa vasorum [ 18 , 19 ] and the SVs surrounding cushion of perivascular fat [ 20 , 21 ] . The damage inflicted to the SV using both OVH and EVH affects both short- and long-term graft performance.…”

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confidence: 99%

Endoscopic or No-Touch Vein Harvesting for CABG: What is Best for the Patient?

Kopjar¹,

Ivanković²,

Lima³

et al. 2016

Brazilian Journal of Cardiovascular Surgery

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Avaliação microscópica e ultra-estrutural do endotélio de veia safena preparada pela técnica "no touch"

Abstract: Avaliação microscópica e ultra-estrutural do endotélio de veia safena preparada pela técnica "no touch"Microscopic and ultrastructural evaluation of the saphenous vein endothelium for CABG prepared by the no touch technique

Cited by 7 publications

References 16 publications

Transforming growth factor-β in graft vessels: histology and immunohistochemistry

Transforming growth factor-β in graft vessels: histology and immunohistochemistry

Impacto das publicações científicas brasileiras nos recentes avanços científicos na cardiologia

Endoscopic or No-Touch Vein Harvesting for CABG: What is Best for the Patient?

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