Sulfated polysaccharide extracted of the green algae Caulerpa racemosa increase the enzymatic activity and paw edema induced by sPLA2 from Crotalus durissus terrificus venom

Pires, Camila Lehnhardt; Rodrigues, Samuel Filgueiras; Bristot, Daniel; Gaeta, Henrique Hessel; Toyama, Daniela de Oliveira; Farias, Wladimir Ronald Lobo; Toyama, Marcos Hikari

doi:10.1590/s0102-695x2013005000050

Cited by 9 publications

(4 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When native sPLA2 (14 kDa) was incubated with CrSP, the compounds formed a stable molecular complex (sPLA2:CrSP) with a Mw of approximately 32 kDa. CrSP caused significant increases in sPLA2 enzymatic and bactericidal activity and edematogenic effects in a pharmacological assay with an animal model because of this stable molecular complex [ 100 ].…”

Section: Biological Activities Of Sulfated Polysaccharides Of Greementioning

confidence: 99%

Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years

et al. 2014

View full text Add to dashboard Cite

Among the three main divisions of marine macroalgae (Chlorophyta, Phaeophyta and Rhodophyta), marine green algae are valuable sources of structurally diverse bioactive compounds and remain largely unexploited in nutraceutical and pharmaceutical areas. Recently, a great deal of interest has been developed to isolate novel sulfated polysaccharides (SPs) from marine green algae because of their numerous health beneficial effects. Green seaweeds are known to synthesize large quantities of SPs and are well established sources of these particularly interesting molecules such as ulvans from Ulva and Enteromorpha, sulfated rhamnans from Monostroma, sulfated arabinogalactans from Codium, sulfated galacotans from Caulerpa, and some special sulfated mannans from different species. These SPs exhibit many beneficial biological activities such as anticoagulant, antiviral, antioxidative, antitumor, immunomodulating, antihyperlipidemic and antihepatotoxic activities. Therefore, marine algae derived SPs have great potential for further development as healthy food and medical products. The present review focuses on SPs derived from marine green algae and presents an overview of the recent progress of determinations of their structural types and biological activities, especially their potential health benefits.

show abstract

Section: Biological Activities Of Sulfated Polysaccharides Of Greementioning

confidence: 99%

Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The incubations of C. d. terrificus sPLA2 with purified 8CR (mol/mol) were performed according to the procedure described previously [ 21 ]. The results of reverse phase HPLC analysis showed that 8CR could form a stable complex with sPLA2, and that 8CR also changed the molecular structure of sPLA2, since the retention time of sPLA2 changed significantly in the presence of 8CR ( Figure 2 A).…”

Section: Resultsmentioning

confidence: 99%

Non-Clinical Studies for Evaluation of 8-C-Rhamnosyl Apigenin Purified from Peperomia obtusifolia against Acute Edema

Tamayose

Romoff

Toyama

et al. 2017

IJMS

View full text Add to dashboard Cite

Compound 8-C-rhamnosyl apigenin (8CR) induced a moderate reduction in the enzymatic activity of secretory phospholipase A2 (sPLA2) from Crotalus durissus terrificus and cytosolic phospholipase A2 (cPLA2), but the compound also significantly inhibited the enzymatic activity of the enzyme cyclooxygenase. In vitro assays showed that the compound induced a slight change in the secondary structure of sPLA2 from Crotalus durissus terrificus snake venom. In vivo assays were divided into two steps. In the first step, the 8CR compound was administered by intraperitoneal injections 30 min prior to administration of sPLA2. In this condition, 8CR inhibited edema and myonecrosis induced by the sPLA2 activity of Crotalus durissus terrificus in a dose-dependent manner by decreasing interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α), prostaglandin E2 (PGE2), and lipid peroxidation. This has been demonstrated by monitoring the levels of malondialdehyde (MDA) in rat paws after the course of edema induced by sPLA2. These results, for the first time, show that sPLA2 of Crotalus durissus terrificus venom induces massive muscle damage, as well as significant edema by mobilization of cyclooxygenase enzymes. Additionally, its pharmacological activity involves increased lipid peroxidation as well as TNF-α and IL-1β production. Previous administration by the peritoneal route has shown that dose-dependent 8CR significantly decreases the enzymatic activity of cyclooxygenase enzymes. This resulted in a decrease of the amount of bioactive lipids involved in inflammation; it also promoted a significant cellular protection against lipid peroxidation. In vivo experiments performed with 8CR at a concentration adjusted to 200 μg (8 mg/kg) of intraperitoneal injection 15 min after sPLA2 injection significantly reduced sPLA2 edema and the myotoxic effect induced by sPLA2 through the decrease in the enzymatic activity of cPLA2, cyclooxygenase, and a massive reduction of lipid peroxidation. These results clearly show that 8CR is a potent anti-inflammatory that inhibits cyclooxygenase-2 (COX-2), and it may modulate the enzymatic activity of sPLA2 and cPLA2. In addition, it was shown that Crotalus durissus terrificus sPLA2 increases cell oxidative stress during edema and myonecrosis, and the antioxidant properties of the polyphenolic compound may be significant in mitigating the pharmacological effect induced by sPLA2 and other snake venom toxins.

show abstract

“…2F. It behaves as a shadeadapted species with a low photoprotective capacity due to light is one of the main factors governing its distribution 74,75 .…”

Section: Caulerpa Proliferamentioning

confidence: 99%

Untitled

2019

IJPSR

View full text Add to dashboard Cite

From the beginning of civilization, humans depend on the natural products for their food and as medical treatment. Diversity in various bioactive compounds from marine organisms has been tested for the multiple pharmacological effects. Marine algae are surviving in a competitive environment with developed defense strategies and significant levels of chemical structural diversity with a wide variety of bioactive secondary metabolites. Therefore, algae are a promising source of novel active biochemical compounds with different pharmacological properties for the prospective development of novel drugs by the pharmaceutical industries. Microalgae (blue-green, dinoflagellates, bacillariophyte, etc.) and Macroalgae (green, brown and red algae) are the two groups of algae. Caulerpa is the genera of green macroalgae, represent the numerous bioactive compounds like tannin, steroids, flavonoids, terpenoids, sulphated polysaccharides (SP), glycosides, phenolic compounds, and saponins, etc., for significant pharmacological effects in the current medicine. Exploration of these compounds for the pharmaceutical and medical purpose, the present review focused on morphological behavior and pharmacological activities of some Caulerpa species in current drug research.

show abstract

Sulfated polysaccharide extracted of the green algae Caulerpa racemosa increase the enzymatic activity and paw edema induced by sPLA2 from Crotalus durissus terrificus venom

Cited by 9 publications

References 41 publications

Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years

Overview on Biological Activities and Molecular Characteristics of Sulfated Polysaccharides from Marine Green Algae in Recent Years

Non-Clinical Studies for Evaluation of 8-C-Rhamnosyl Apigenin Purified from Peperomia obtusifolia against Acute Edema

Untitled

Contact Info

Product

Resources

About