Wound healing potential of aqueous crude extract of Stevia rebaudiana in mice

Das, Kuntal

doi:10.1590/s0102-695x2013005000011

Cited by 38 publications

(22 citation statements)

References 16 publications

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“…Wound healing rate was significantly higher (p ≤ 0.05) in THC-SLNs gel treated animals than those treated with free THC gel. THC-SLNs gel left no prominent scar at the wound site, pointing towards the enhanced bioactivity of THC post incorporation into SLNs62 .Latter was confirmed by the histological investigations, which revealed a complete healing of skin on application of THC-SLNs gel. Similar study reported byRao et al (2015) reported the usefulness of glucosylated THC in the process of wound healing63 .…”

mentioning

confidence: 63%

Topical delivery of tetrahydrocurcumin lipid nanoparticles effectively inhibits skin inflammation:in vitroandin vivostudy

Kakkar

Kaur

et al. 2018

Drug Development and Industrial Pharmacy

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Tetrahydrocurcumin (THC) also referred to as 'white curcumin', is a stable colorless hydrogenated product of curcumin with superior antioxidant and anti-inflammatory properties. The present study is an attempt to elevate the topical bioavailability of THC, post-incorporation into a nano-carrier system with its final dosage as a hydrogel. Lipid nanoparticles of THC (THC-SLNs) prepared by microemulsification technique were ellipsoidal in shape (revealed in transmission electron microscopy) with a mean particle size of 96.6 nm and zeta potential of -22 mV. Total drug content and entrapment efficiency of THC-SLNs was 94.51% ± 2.15% and 69.56% ± 1.35%, respectively. Differential scanning calorimetry and X-ray diffraction studies confirmed the formation of THC-SLNs. In vitro drug release studies showed the drug release from THC-SLNs gel to follow Higuchi's equation revealing a Fickian diffusion. Ex vivo permeation studies indicated a 17 times (approximately) higher skin permeation of THC-SLNs gel as compared with the free THC gel. Skin irritation, occlusion, and stability studies indicated the formulation to be nonirritating, and stable with a desired occlusivity. Pharmacodynamic evaluation in an excision wound mice model clearly revealed the enhanced anti-inflammatory activity of THC-SLNs gel and was further confirmed using biochemical and histopathological studies. It is noteworthy to report here that THC-SLNs gel showed significantly better (p ≤ 0.001) activity than free THC in gel. As inflammation is innate to all the skin disorders, the developed product opens up new therapeutic avenues for several skin diseases. To the best of our knowledge, this is the first paper elaborating the therapeutic usefulness of white curcumin-loaded lipidic nanoparticles for skin inflammation.

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mentioning

confidence: 63%

Topical delivery of tetrahydrocurcumin lipid nanoparticles effectively inhibits skin inflammation:in vitroandin vivostudy

Kakkar

Kaur

et al. 2018

Drug Development and Industrial Pharmacy

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“…The dorsal skin area of the test animals was shaved prior to the incision. Wounds (10 × 6 mm average) were cut on each mouse under sterile conditions (Karodi et al, 2009 ; Das, 2013 ). Chloroform was used for anesthesia to minimize pain and stress to the animals as a result of the wounding processes.…”

Section: Methodsmentioning

confidence: 99%

Wound Healing Properties of Selected Plants Used in Ethnoveterinary Medicine

et al. 2017

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Plants have arrays of phytoconstituents that have wide ranging biological effects like antioxidant, anti-inflammatory and antimicrobial properties key in wound management. In vivo wound healing properties of ointments made of crude methanolic extracts (10% extract w/w in white soft paraffin) of three plant species, Cissus quadrangularis L. (whole aerial plant parts), Adenium multiflorum Klotzsch (whole aerial plant parts) and Erythrina abyssinica Lam. Ex DC. (leaves and bark) used in ethnoveterinary medicine were evaluated on BALB/c female mice based on wound area changes, regular observations, healing skin's percentage crude protein content and histological examinations. White soft paraffin and 3% oxytetracycline ointment were used as negative and positive controls, respectively. Wound area changes over a 15 day period for mice treated with C. quadrangularis and A. multiflorum extract ointments were comparable to those of the positive control (oxytetracycline ointment). Wounds managed with the same extract ointments exhibited high crude protein contents, similar to what was observed on animals treated with the positive control. Histological evaluations revealed that C. quadrangularis had superior wound healing properties with the wound area completely returning to normal skin structure by day 15 of the experiment. E. abyssinica leaf and bark extract ointments exhibited lower wound healing properties though the leaf extract exhibited some modest healing properties.

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“…Where by using the stable DPPH radical, the antioxidant capacity of the extract was measured in terms of hydrogen donating or radical scavenging ability. 1mL of 0.3mM solution of DPPH in ethanol was added to various concentrations of sample (10,20,40,60,80, 100 μg/mL) and the reference compound (5,10,15,20,25 and 30 μg/mL), shaken vigorously, and left to stand in the dark at room temperature. After 30 min absorbance was measured at 517nm against a blank.…”

Section: Invitro Antioxidant Study Of Aqzrmentioning

confidence: 99%

“…Measurement of wound contraction [15] The progression of wound healing was judged by the periodic assessment of the contraction of excision wounds. Wound contraction was monitored by tracing the outline of the wound on tracing sheet and then using graph sheet to calculate the area of the wound size.…”

Section: Estimation Of Parametersmentioning

confidence: 99%

Evaluation of Wound Healing Effect of Aqueous Stem Extract of Zanthoxylum Rhetsa (Roxb.) Dc on Wistar Rat

Abarnadevika¹

2017

WJPR

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The present investigation has been undertaken to study the wound healing properties of aqueous extract of Zanthoxylum rhetsa (AQZR).The plant Zanthoxylum rhetsa has a long history in herbal medicine in many countries. Experiments were conducted following standard procedures. The extract was evaluated for their invitro antioxidant, antimicrobial and total phenol and flavonoid content. The AQZR ointment were administered topically, for evaluating the wound healing potential in excision wound model for twenty one days.Povidone iodine ointment was the standard for excision wound model. Extracts treated group showed in vitro antioxidant, anti microbialproperties as compared with standard and control group. AQZR exhibited similar wound healing activity that of the standard but with lesser magnitude. The result may be attributed to the phytoconstituents such as flavonoids and phenolics present in it which may be due to their individual or cumulative effect that enhanced wound healing and provided scientific evidence to the ethnomedicinal futures of Zanthoxylum rhetsa. These findings could justify the inclusion of this plant in the management of wound healing.

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Wound healing potential of aqueous crude extract of Stevia rebaudiana in mice

Cited by 38 publications

References 16 publications

Topical delivery of tetrahydrocurcumin lipid nanoparticles effectively inhibits skin inflammation:in vitroandin vivostudy

Topical delivery of tetrahydrocurcumin lipid nanoparticles effectively inhibits skin inflammation:in vitroandin vivostudy

Wound Healing Properties of Selected Plants Used in Ethnoveterinary Medicine

Evaluation of Wound Healing Effect of Aqueous Stem Extract of Zanthoxylum Rhetsa (Roxb.) Dc on Wistar Rat

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