Metabolic and biological prospecting of Coreopsis tinctoria

Gaspar, Luís; Oliveira, Andreia P.; Silva, Luís; Andrade, Paula B.; Pinho, Paula Guedes de; Botelho, João; Valentão, Patrı́cia

doi:10.1590/s0102-695x2011005000224

Cited by 13 publications

(13 citation statements)

References 34 publications

(40 reference statements)

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“…A literature search revealed limonene (63.5%), 3-carene (7.0%), p-cymene (5.2%), and zingiberene (2.4%) to be the main constituents of EO from the wild-growing C. tinctoria flower native to the Kunlun Mountain area, Xinjiang, China [19]. Gaspar et al [12] reported that the major components of C. tinctoria flower EO were α-pinene (45.2%) and trans-carve (28.6 %). These variations in the chemical constituents of the EO may be attributed to the various agro climatic (geographical, climatical, and seasonal) conditions of the regions, the adaptive metabolism of plants, and the stage of maturity.…”

Section: Discussionmentioning

confidence: 99%

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Anti-cryptococcus Activity and Mechanism of Essential Oil from Coreopsis tinctoria Flowering on Cryptococcus

Zeng¹,

Li²,

Ding³

et al. 2016

Fungal Genom Biol

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The essential oil (EO) extracted from the flower of Coreopsis tinctoria Nutt. is a kind of plant-derived natural therapeutics against Cryptococcus neoformans. In our study, the anti-cryptococcal effects of the EO were observed from various aspects, such as minimal inhibitory concentration, minimum fungicidal concentration, germ tube formation, and biomass. The results indicated that the EO had potent antifungal activities on C. neoformans and that it was more effective than the commonly used antifungal agents fluconazole and amphotericin B. To further elucidate the mechanism of the antifungal action, the effect of the EO on the plasma membrane and the mitochondria of C. neoformans were investigated. The lesion in the plasma membrane was detected through flow cytometry and was further verified through the inhibition of ergosterol synthesis. The essential oil caused morphological changes in the cells of C. neoformans and a reduction in the ergosterol quantity. Moreover, mitochondrial membrane potential, mitochondrial ATPase, succinate dehydrogenase (SDH), and malate dehydrogenase (MDH) were detected. The reactive oxygen species (ROS) accumulation was examined through fluorometric assay. Decreased SDH, MDH, and ATPase in C. neoformans cells were also observed in a dose-dependent manner. The above dysfunctions of the mitochondria caused ROS accumulation in C. neoformans. A reduction in cell viability was prevented through the addition of L-cysteine, and this reduction indicates that ROS is an important mediator of the antifungal action of the EO. In summary, EO from C. tinctoria flower showed anti-cryptococcus activity. Its mechanism of action is to disrupt the permeability barrier of the plasma membrane and the mitochondrial dysfunction-induced ROS accumulation in C. neoformans. With better antifungal activity than the commonly used antifungal agents and less possibility to induce drug resistance, the EO can be used as a potential anti-cryptococcal agent.

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Section: Discussionmentioning

confidence: 99%

“…C. tinctoria is widely used not only as an ornamental plant but also as functional food in China. It is rich in essential nutrients (e.g., vitamins, selenium, zinc, and amino acids [11,12], etc.) and many phytochemicals (e.g., flavonoids, polyphenols, polysaccharides, saponins, and EO) [13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Anti-cryptococcus Activity and Mechanism of Essential Oil from Coreopsis tinctoria Flowering on Cryptococcus

Zeng¹,

Li²,

Ding³

et al. 2016

Fungal Genom Biol

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“…Therefore, as limonene is known for its antitumorigenic effects [11], this species of Coreopsis tinctoria could be tested for its antitumoral activity. In addition, the relatively high concentration of p-cymene together with important concentrations of pinene and terpinene ensures that this plant possesses antibacterial properties, as was reported by Gaspar and co-workers [7]. …”

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confidence: 53%

“…A critical search of the literature reveals that there are few papers dedicated to the Coreopsis genus, and most of them focused on the chemical composition of the petals [5,6]. So far, only one paper described a systematic study on the metabolic and biological properties of Coreopsis tinctoria [7].Considered an alien species in Romania, this plant is cultivated mostly for its decorative value and has been classified among the new and rare plants in Romanian flora [8]. Therefore, because of its interesting properties, Coreopsis tinctoria cultivated in Romania was subjected to a systematic study regarding its chemical composition.…”

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Chemical Composition of the Aerial Part and Fruits of Coreopsis tinctoria

et al. 2015

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Coreopsis tinctoria Nutt. from the Asteraceae family is an annual plant originating from North America and is widespread all over the world [1]. It is cultivated for both its ornamental and dyeing properties because of its bright yellow daisy-like flowers with maroon centers. Recently, this plant has been used to treat several disorders including diarrhea, internal pain and bleeding, to strengthen blood, to control diabetes, and also as an emetic [2][3][4]. A critical search of the literature reveals that there are few papers dedicated to the Coreopsis genus, and most of them focused on the chemical composition of the petals [5,6]. So far, only one paper described a systematic study on the metabolic and biological properties of Coreopsis tinctoria [7].Considered an alien species in Romania, this plant is cultivated mostly for its decorative value and has been classified among the new and rare plants in Romanian flora [8]. Therefore, because of its interesting properties, Coreopsis tinctoria cultivated in Romania was subjected to a systematic study regarding its chemical composition. Thus, this study reports on the identification of the major volatile compounds found in both the dried fruits and the aerial parts of this plant.Plant Material. Coreopsis tinctoria plants were cultivated in Bucharest and their aerial parts were collected during the flowering period, while fruits were collected at maturity. The plant material was air-dried and further stored in hermetically sealed dark paper bags to protect them from humidity and light. The Coreopsis tinctoria plant was authenticated by botanists from the Bucharest Botanical Garden based on available literature [9].Headspace GC/MS Analysis. A quantity of 0.5 g of dry plant material (whether aerial part or fruits) was placed in a 20 mL headspace vial sealed with a silicone rubber septum and aluminum cap. Gas chromatography was carried out using a Thermo Electron apparatus fitted with a Triplus headspace automatic sampler. A DB-5MS capillary column (25 m u 0.25 mm; 0.25 Pm film thickness) was used. The GC oven temperature program was: initial temperature 60qC (3 min) followed by an increase of 10qC/min up to 200qC (2 min) and then 12qC/min to the final temperature of 240qC (2 min). The carrier gas (helium) flow rate was 1 mL/min. The headspace temperature was kept at 80qC for 10 min prior to the injection of 500 PL of the headspace gas into the column. The source and interface temperatures were 200 and 250qC, respectively. The detector operated in the electron impact mode (70 eV). Detection was performed in the range of m/z 35-300. The mass spectrometer was operated in the full-scan mode. All peaks of the chromatograms were analyzed using Xcalibur® software and the NIST 11 Mass Spectral Library to identify the corresponding compounds. An alkane standard solution for GC (C 8 -C 20 in hexane) was used for retention index (RI) calculations [10]. The relative percent of individual components was calculated based on GC peak areas.GC/MS analysis of both the aerial part of the ...

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