Antiviral activity of Ageratina havanensis and major chemical compounds from the most active fraction

Barrio, Gloria del; Spengler, Iraida; García, T.; Roque, Annele; Álvarez, Ángel L.; Calderón, José S.; Parra, Francisco

doi:10.1590/s0102-695x2011005000159

Cited by 21 publications

(14 citation statements)

References 24 publications

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“…Several studies suggest the potential antiherpetic effect of flavonoids . The antiviral effect of kaempferol against HSV‐1 and HSV‐2 was previously demonstrated .…”

Section: Resultsmentioning

confidence: 96%

Anti‐HSV‐1 and HSV‐2 Flavonoids and a New Kaempferol Triglycoside from the Medicinal Plant Kalanchoe daigremontiana

Ürményi

Saraiva

Casanova

et al. 2016

Chemistry & Biodiversity

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Kalanchoe daigremontiana (Crassulaceae) is a medicinal plant native to Madagascar. The aim of this study was to investigate the flavonoid content of an aqueous leaf extract from K. daigremontiana (Kd), and assess its antiherpetic potential. The major flavonoid, kaempferol 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside (1), was isolated from the AcOEt fraction (Kd-AC). The BuOH-soluble fraction afforded quercetin 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside (2) and the new kaempferol 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside-7-O-β-d-glucopyranoside (3), named daigremontrioside. The crude extract, Kd-AC fraction, flavonoids 1 and 2 were evaluated using acyclovir-sensitive strains of HSV-1 and HSV-2. Kd-AC was highly active against HSV-1 (EC = 0.97 μg/ml, SI > 206.1) and HSV-2 (EC = 0.72 μg/ml, SI > 277.7). Flavonoids 1 and 2 showed anti-HSV-1 (EC = 7.4 μg/ml; SI > 27 and EC = 5.8 μg/ml; SI > 8.6, respectively) and anti-HSV-2 (EC = 9.0 μg/ml; SI > 22.2 and EC = 36.2 μg/ml; SI > 5.5, respectively) activities, suggesting the contribution of additional substances to the antiviral activity.

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“…Several studies suggest the potential antiherpetic effect of flavonoids . The antiviral effect of kaempferol against HSV‐1 and HSV‐2 was previously demonstrated .…”

Section: Resultsmentioning

confidence: 96%

Anti‐HSV‐1 and HSV‐2 Flavonoids and a New Kaempferol Triglycoside from the Medicinal Plant Kalanchoe daigremontiana

Ürményi

Saraiva

Casanova

et al. 2016

Chemistry & Biodiversity

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“…First, the cytotoxic effects of Myt-C and Myt-Tat on Vero cells were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay, as previously described (28). Briefly, increasing concentrations of the test samples were added to confluent Vero cells in 96-well plates, with six replicates per concentration.…”

Section: Methodsmentioning

confidence: 99%

Antiviral Activity of Myticin C Peptide from Mussel: an Ancient Defense against Herpesviruses

Novoa

Romero

Álvarez

et al. 2016

J Virol

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Little is known about the antiviral response in mollusks. As in other invertebrates, the interferon signaling pathways have not been identified, and in fact, there is a debate about whether invertebrates possess antiviral immunity similar to that of vertebrates. In marine bivalves, due to their filtering activity, interaction with putative pathogens, including viruses, is very high, suggesting that they should have mechanisms to address these infections. In this study, we confirmed that constitutively expressed molecules in naive mussels confer resistance in oysters to ostreid herpesvirus 1 (OsHV-1) when oyster hemocytes are incubated with mussel hemolymph. Using a proteomic approach, myticin C peptides were identified in both mussel hemolymph and hemocytes. Myticins, antimicrobial peptides that have been previously characterized, were constitutively expressed in a fraction of mussel hemocytes and showed antiviral activity against OsHV-1, suggesting that these molecules could be responsible for the antiviral activity of mussel hemolymph. For the first time, a molecule from a bivalve has shown antiviral activity against a virus affecting mollusks. Moreover, myticin C peptides showed antiviral activity against human herpes simplex viruses 1 (HSV-1) and 2 (HSV-2). In summary, our work sheds light on the invertebrate antiviral immune response with the identification of a molecule with potential biotechnological applications. IMPORTANCESeveral bioactive molecules that have potential pharmaceutical or industrial applications have been identified and isolated from marine invertebrates. Myticin C, an antimicrobial peptide from the Mediterranean mussel (Mytilus galloprovincialis) that was identified by proteomic techniques in both mussel hemolymph and hemocytes, showed potential as an antiviral agent against ostreid herpesvirus 1 (OsHV-1), which represents a major threat to the oyster-farming sector. Both hemolymph from mussels and a myticin C peptide inhibited OsHV-1 replication in oyster hemocytes. Additionally, a modified peptide derived from myticin C or the nanoencapsulated normal peptide also showed antiviral activity against the human herpesviruses HSV-1 and HSV-2. Therefore, myticin C is an example of the biotechnological and therapeutic potential of mollusks.

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“…The genus Ageratina (Asteraceae) consists of about 1200 species and is distributed in temperate and subtropical regions of the America, Europe, Africa, and Asia [ 1 , 2 , 3 , 4 ]; in Mexico, about 164 species of Ageratina have been reported [ 5 , 6 ]. Several species of this genus have been studied, and these studies have demonstrated bactericidal, antifungal, antiviral, analgesic, cytotoxic, and anti-inflammatory effects, as well as the ability to treat gastric ulcers [ 7 , 8 , 9 , 10 , 11 , 12 ]. In the state of Morelos, Mexico, Ageratina pichinchensis is traditionally used to treat gastric ulcers and heal deep wounds.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Sánchez-Ramos

Álvarez

Romero-Estrada

et al. 2020

Plants

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Ageratina pichinchensis (Kunth) is a plant used in traditional Mexican medicine to treat multiple ailments. However, there have not been biotechnological studies on producing compounds in in vitro cultures. The aim of this study was to establish a cell suspension culture of A. pichinchensis, quantify the anti-inflammatory constituents 2,3-dihydrobenzofuran (2) and 3-epilupeol (3), evaluate the anti-inflammatory potential of its extracts, and perform a phytochemical analysis. Cell suspension cultures were established in a MS culture medium of 30-g L−1 sucrose, 1.0-mg L−1 α-naphthaleneacetic acid, and 0.1-mg L−1 6-furfurylaminopurine. The ethyl acetate extract of the cell culture analyzed by gas chromatography (GC) revealed that the maximum production of anti-inflammatory compounds 2 and 3 occurs on days eight and 16, respectively, improving the time and previously reported yields in callus cultures. The anti-inflammatory activity of these extracts exhibited a significant inhibition of nitric oxide (NO) production. Furthermore, a phytochemical study of the ethyl acetate (EtOAc) and methanol (MeOH) extracts from day 20 led to the identification of 17 known compounds. The structures of the compounds were assigned by an analysis of 1D and 2D NMR data and the remainder by GC–MS. This is the first report of the production of (-)-Artemesinol, (-)-Artemesinol glucoside, encecalin, and 3,5-diprenyl-acetophenone by a cell suspension culture of A. pichinchensis.

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Antiviral activity of Ageratina havanensis and major chemical compounds from the most active fraction

Cited by 21 publications

References 24 publications

Anti‐HSV‐1 and HSV‐2 Flavonoids and a New Kaempferol Triglycoside from the Medicinal Plant Kalanchoe daigremontiana

Anti‐HSV‐1 and HSV‐2 Flavonoids and a New Kaempferol Triglycoside from the Medicinal Plant Kalanchoe daigremontiana

Antiviral Activity of Myticin C Peptide from Mussel: an Ancient Defense against Herpesviruses

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

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