Inhibition of human platelet aggregation in vitro by standardized extract of Wendtia calycina

Abstract

Wendtia calycina (Griseb.) Griseb., Vivianiaceae, is a Paraguayan herbaceous plant commonly known as burrito. Our previous study indicated that burrito leaves are a very good source of phenylpropanoid glycosides, principally verbascoside. From W. calycina leaves, a standardized, water-soluble extract rich in phenylpropanoid glycosides (WSE) has been developed on an industrial scale to be used as a food supplement, cosmetic, phytomedicine, and ingredient of different formulations. In this study, we investigated the effect of the WSE on human platelet aggregation in vitro induced by adenosine diphosphate (ADP), epinephrine (EPN), collagen (COL) or arachidonic acid (AA). WSE, concentration-dependently, inhibited ADP and EP-induced human platelet aggregation (IC50 were 0.82±0.15 mg/mL and 0.41±0.02 mg/mL, respectively). It did not inhibit collagen-induced platelet aggregation, thus suggesting a selectivity for the ADP-induced platelet activation pathways.

Preparations from selected cucurbit vegetables as antiplatelet agents

“…Preparations from pattypan squash are include a range of phenolic derivates, many of which show anti-platelet activity. One of the main compounds found in pattypan squash was phenylpropanoid glycoside, known to demonstrate anti-platelet activity 33 . Mesa et al report that Wendtia calycina extract significantly reduced platelet aggregation induced by collagen in platelet-rich plasma in vitro.…”

“…Mesa et al report that Wendtia calycina extract significantly reduced platelet aggregation induced by collagen in platelet-rich plasma in vitro. This extract contains a high level of phenylpropanoid glycoside, and a certain amount of benzoic acid derivates 33 , both of which were identified in pattypan squash (Table 1 ), with the benzoic acid derivative in the pattypan squash demonstrating a strong anti-aggregation effect 33 . The batch variability of plant matter is connected with the quantitative differences in chemical composition, due to the various plant cultivation methods and soil quality.…”

Preparations from Selected Cucurbit Vegetables as Antiplatelet Agents – An in Vitro Study

“…Mesa et al report that Wendtia calycina extract signi cantly reduced platelet aggregation induced by collagen in platelet-rich plasma in vitro. This extract contains a high level of phenylpropanoid glycoside, and a certain amount of benzoic acid derivates 31 , both of which were identi ed in pattypan squash (Table 1), with the benzoic acid derivative in the pattypan squash demonstrating a strong anti-aggregation effect 31 .…”