Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta

Yokoya, Nair S.; Yoneshigue‐Valentin, Yocie

doi:10.1590/s0102-695x2011005000077

Cited by 31 publications

(12 citation statements)

References 20 publications

(28 reference statements)

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“…This is consistent with previously published results demonstrating wide variation in concentrations of IAA and other auxin metabolites, such as IAA-Glu, IAA-Leu and IAM, in cyanobacteria and algae (Tarakhovskaya et al , 2007; Hussain et al , 2010; Yokoya et al , 2010; Yokoya and Yoneshigue-Valentin, 2011). The auxin derivatives OxIAA and IAA-Asp detected in our study represent metabolic products of two major catabolic pathways of IAA in plants.…”

Section: Discussionsupporting

confidence: 93%

Control of cytokinin and auxin homeostasis in cyanobacteria and algae

Žižková

Kubeš

Dobrev

et al. 2016

Ann Bot

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Background and Aims The metabolism of cytokinins (CKs) and auxins in vascular plants is relatively well understood, but data concerning their metabolic pathways in non-vascular plants are still rather rare. With the aim of filling this gap, 20 representatives of taxonomically major lineages of cyanobacteria and algae from Cyanophyceae, Xanthophyceae, Eustigmatophyceae, Porphyridiophyceae, Chlorophyceae, Ulvophyceae, Trebouxiophyceae, Zygnematophyceae and Klebsormidiophyceae were analysed for endogenous profiles of CKs and auxins and some of them were used for studies of the metabolic fate of exogenously applied radiolabelled CK, [3H]trans-zeatin (transZ) and auxin ([3H]indole-3-acetic acid (IAA)), and the dynamics of endogenous CK and auxin pools during algal growth and cell division.Methods Quantification of phytohormone levels was performed by high-performance or ultrahigh-performance liquid chromatography–electrospray tandem mass spectrometry (HPLC-MS/MS, UHPLC-MS/MS). The dynamics of exogenously applied [3H]transZ and [3H]IAA in cell cultures were monitored by HPLC with on-line radioactivity detection.Key Results The comprehensive screen of selected cyanobacteria and algae for endogenous CKs revealed a predominance of bioactive and phosphate CK forms while O- and N-glucosides evidently did not contribute greatly to the total CK pool. The abundance of cis-zeatin-type CKs and occurrence of CK 2-methylthio derivatives pointed to the tRNA pathway as a substantial source of CKs. The importance of the tRNA biosynthetic pathway was proved by the detection of tRNA-bound CKs during the course of Scenedesmus obliquus growth. Among auxins, free IAA and its oxidation catabolite 2-oxindole-3-acetic acid represented the prevailing endogenous forms. After treatment with [3H]IAA, IAA-aspartate and indole-3-acetyl-1-glucosyl ester were detected as major auxin metabolites. Moreover, different dynamics of endogenous CKs and auxin profiles during S. obliquus culture clearly demonstrated diverse roles of both phytohormones in algal growth and cell division.Conclusions Our data suggest the existence and functioning of a complex network of metabolic pathways and activity control of CKs and auxins in cyanobacteria and algae that apparently differ from those in vascular plants.

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Section: Discussionsupporting

confidence: 93%

Control of cytokinin and auxin homeostasis in cyanobacteria and algae

Žižková

Kubeš

Dobrev

et al. 2016

Ann Bot

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“…Proses kultur in vitro dapat dipengaruhi oleh faktor endogen dan eksogen. Faktor endogen meliputi karakteristik eksplan seperti umur, sumber, tahap perkembangan dan fisiologi, sedangkan faktor eksogen meliputi salinitas, iradiasi, fotoperiodisasi, temperatur, pH dan komposisi media [9]. Laju pertumbuhan tinggi dapat dicapai melalui pengamatan dan pengendalian faktor abiotik yang diketahui berpengaruh terhadap pertumbuhan spesies [10].…”

Section: Pendahuluanunclassified

Laju Pertumbuhan Eksplan Rumput Laut (Gracilaria verrucosa (Hudson) Papenfuss) Secara In vitro

Nurrahmawan

Jadid

2017

JSSITS

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Pada tahun 2016, kebutuhan Agar dunia mencapai 550.000 ton dan terus meningkat tiap tahunnya. Peningkatan kebutuhan tersebut harus diiringi dengan peningkatan produksi rumput laut. Gracilaria verrucosa merupakan rumput laut penghasil Agar dan banyak dibudidayakan di Indonesia. Akan tetapi, produksi rumput laut G. verrucosa masih rendah disebabkan oleh laju pertumbuhan yang lambat. Selain itu, penyediaan bibit unggul juga masih sulit dilakukan. Teknik kultur in vitro melalui penambahan ZPT auksin dan sitokinin diketahui dapat meningkatkan laju pertumbuhan eksplan. Oleh karena itu, penelitian bertujuan untuk mengetahui pengaruh kombinasi ZPT IAA dan BAP terhadap laju pertumbuhan rumput laut G. verrucosa secara in vitro. Penelitian dilakukan dengan menumbuhkan eksplan interkalar G. verrucosa ke dalam medium PES dengan penambahan kombinasi IAA dan BAP (0; 0,1; 0,3; 0,5 mg/l) selama 30 hari. Hasil penelitian menunjukkan kombinasi IAA dan BAP berpengaruh terhadap laju pertumbuhan eksplan rumput laut G. verrucosa. Perlakuan IAA 0 mg/l dan BAP 0,5 mg/l menunjukkan pengaruh tertinggi terhadap laju pertumbuhan yakni sebesar 0,42% per hari.

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“…There is an increasing attention for in vitro cultivation of macroalgae; however, it lacks the theoretical and practical know‐how in comparison with land‐based plant systems. The in vitro cultures have been reported to be applicable for axenic production of micro plantlets and sometimes for callus regeneration and clonal propagation of macroalgae cells . The idea of using axenic seaweed cultures started to develop macroalgae production in lab scale experiments.…”

Section: Algal Bioprocesses In Large Scalesmentioning

confidence: 99%

Algae as a promising resource for biofuel industry: facts and challenges

Köse

Öncel

2016

Int. J. Energy Res.

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Summary Energy is the main driving force of society today that should be handled as a whole starting from production to consumption. With the rapid increase in the energy necessity, alternative methods and sources are becoming a crucial topic that should be scientifically highlighted with all their pros and cons. Especially the problems related to the fossil sources of energy triggered the search on the renewable alternatives like algae. In order to reach the desired amounts of energy with the satisfactory quality and quantity, understanding the algae as a living thing with the biological mechanism and existing production technologies are the key points to have a projection for commercialization. In this regard, technical facts and challenges on algal biofuel production should be evaluated. Keeping in mind the specifications and possible advantages related to their taxonomy, algae can serve as a promising source to reduce fossil fuel consumption. With the progress in the modern technology, reaching an effective production process will be possible, and this will help the algal biofuels to prove their maturity as a sustainable source for future. Within this context, the aim of this review is to point out the crucial technical challenges about algal fuels comprising both the macroalgae and microalgae as a reliable source of renewable energy. Copyright © 2016 John Wiley & Sons, Ltd.

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Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta

Cited by 31 publications

References 20 publications

Control of cytokinin and auxin homeostasis in cyanobacteria and algae

Control of cytokinin and auxin homeostasis in cyanobacteria and algae

Laju Pertumbuhan Eksplan Rumput Laut (Gracilaria verrucosa (Hudson) Papenfuss) Secara In vitro

Algae as a promising resource for biofuel industry: facts and challenges

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