Development and validation of a HPLC method for standardization of herbal and commercial extracts of Myrcia uniflora

Batista, Andrea Nastri de Luca; Colombo, Renata; Pascoli, Inara C. de; Teles, Helder Lopes; Silva, Geraldo Humberto; Bomfim, Giovanni C. C.; Burgos, Rosilene Cristina Rossetto; Cavalheiro, Alberto José; Bolzani, Vanderlan da Silva; Silva, Dulce Helena Siqueira; Reimberg, Maria Célia H.

doi:10.1590/s0102-695x2011005000066

Cited by 5 publications

(3 citation statements)

References 10 publications

(16 reference statements)

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“…[49], M. splendens [50], M. palustris DC. [50] and M. uniflora [51], mearnsitrin ( 7 ) was also obtained from M. uniflora [52]. From the leaves of M. tomentosa , avicularin ( 11 ) and juglanin ( 12 ) were isolated [53].…”

Section: Non-volatilesmentioning

confidence: 99%

Constituents and Pharmacological Activities of Myrcia (Myrtaceae): A Review of an Aromatic and Medicinal Group of Plants

Cascaes

Guilhon

Andrade

et al. 2015

IJMS

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Myrcia is one of the largest genera of the economically important family Myrtaceae. Some of the species are used in folk medicine, such as a group known as “pedra-hume-caá” or “pedra-ume-caá” or “insulina vegetal” (insulin plant) that it is used for the treatment of diabetes. The species are an important source of essential oils, and most of the chemical studies on Myrcia describe the chemical composition of the essential oils, in which mono- and sesquiterpenes are predominant. The non-volatile compounds isolated from Myrcia are usually flavonoids, tannins, acetophenone derivatives and triterpenes. Anti-inflammatory, antinociceptive, antioxidant, antimicrobial activities have been described to Myrcia essential oils, while hypoglycemic, anti-hemorrhagic and antioxidant activities were attributed to the extracts. Flavonoid glucosides and acetophenone derivatives showed aldose reductase and α-glucosidase inhibition, and could explain the traditional use of Myrcia species to treat diabetes. Antimicrobial and anti-inflammatory are some of the activities observed for other isolated compounds from Myrcia.

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Section: Non-volatilesmentioning

confidence: 99%

Constituents and Pharmacological Activities of Myrcia (Myrtaceae): A Review of an Aromatic and Medicinal Group of Plants

Cascaes

Guilhon

Andrade

et al. 2015

IJMS

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“…соответствовали 3-О-рамнозиду мирицетина (мирицитрину) (Batista et al, 2011), обнаруженному во многих видах рода Rhododendron (Komissarenko, Levashova, 1980;Yang et al, 2009;Jaiswal et al, 2012;Mok, Lee, 2013).…”

Section: таблица 2 размеры особей и листовых пластинок Rhododendron Punclassified

Ecological and geographical variation of morphometric parameters and flavonoid composition of Rhododendron parvifolium

2018

turczaninowia

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Ключевые слова: кластерный анализ, морфометрия, популяции, флавоноиды, Rhododendron parvifolium.Аннотация. Rhododendron parvifolium обладает обширным ареалом в Восточной Сибири и Дальнем Вос-токе России, включая остров Сахалин. Значительный полиморфизм морфологических и химических призна-ков вида слабо изучен. Нами проведено изучение морфологических показателей и состава флавоноидов в образцах растений из четырех популяций этого вида, в том числе растений из популяции, расположенной в Иркутской области (г. Слюдянка), отличающейся от остальных значительной высотой растений и размерами листьев. Климат в месте ее произрастания был значительно более мягким по сравнению с местообитаниями остальных популяций. Определяли длину и ширину листовой пластинки, длину черешка, листовой индекс -отношение ширины к длине листа. В результате кластерного анализа морфометрических данных выявлено обособление популяции из Иркутской области от остальных, образующих единый кластер. Методом высоко-эффективной жидкостной хроматографии в гидролизатах образцов удалось идентифицировать пять агликонов (дигидрокверцетин, мирицетин, кверцетин, лютеолин и кемпферол), главными из которых были кверцетин и ди-гидрокверцетин. Методом высокоэффективной жидкостной хроматографии-масс-спектрометрии в нативных экстрактах всех образцов идентифицированы гиперозид, авикулярин, а также пентозиды дигидрокверцетина и кверцетина, дезоксигексозид дигидрокверцетина, кверцетин и кемпферол в свободном виде. Основными гликозидными компонентами во всех образцах были пентозид дигидрокверцетина и гиперозид. Кластерный анализ данных по составу флавоноидов показал наличие двух кластеров, характеризующихся различными эдафическими условиями. Популяции, произрастающие на гольцах, отличались от растущих на болотах по-вышенным содержанием флавоноидов, в том числе гликозидов дигидрокверцетина, мирицетина, кверцетина, а также свободных агликонов.

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“…The application of the validated UHPLC-UV/PDA method for simultaneous flavonoid quantification has been established for various plant extracts, including Dracocephalum heterophyllum Benth. [ 13 ], Myrcia uniflora [ 14 ], Dimorphandra gardneriana [ 15 ], Trigonella stellate [ 11 ], and many others [ 16 , 17 , 18 , 19 , 20 , 21 ]. The identification of flavonoid C -glycosides in OPL via UHPLC-UV/PDA-MS/MS analysis has been described in our previous works [ 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

UHPLC-UV/PDA Method Validation for Simultaneous Quantification of Luteolin and Apigenin Derivatives from Elaeis guineensis Leaf Extracts: An Application for Antioxidant Herbal Preparation

et al. 2021

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Luteolin and apigenin derivatives present in oil palm (Elaeis guineensis) leaves (OPL) are reported to possess excellent antioxidant properties relating to numerous health benefits. To meet the global demand for flavonoids, OPL, which is plentifully generated as an agricultural by-product from oil palm plantations, can be further exploited as a new source of natural antioxidant compounds. However, to produce a standardized herbal preparation, validation of the quantification method for these compounds is required. Therefore, in this investigation, we developed and validated an improved and rapid analytical method, ultra-high-performance liquid chromatography equipped with ultraviolet/photodiode array (UHPLC-UV/PDA) for the quantification of 12 luteolin and apigenin derivatives, particularly focusing on flavonoid isomeric pairs: orientin/isoorientin and vitexin/isovitexin, present in various OPL extracts. Several validation parameters were assessed, resulting in the UHPLC-UV/PDA technique offering good specificity, linearity, accuracy, precision, and robustness, where the values were within acceptable limits. Subsequently, the validated method was employed to quantify luteolin and apigenin derivatives from OPL subjected to different drying treatments and extraction with various solvent systems, giving total luteolin (TLC) and apigenin content (TAC) in the range of 2.04–56.30 and 1.84–160.38 µg/mg extract, respectively. Additionally, partial least square (PLS) analysis disclosed the combination of freeze dry-aqueous methanol yielded OPL extracts with high TLC and TAC, which are strongly correlated with antioxidant activity. Therefore, we provide the first validation report of the UHPLC-UV/PDA method for quantification of luteolin and apigenin derivatives present in various OPL extracts, suggesting that this approach could be employed in standardized herbal preparations by adopting orientin, isoorientin, vitexin, and isovitexin as chemical markers.

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Development and validation of a HPLC method for standardization of herbal and commercial extracts of Myrcia uniflora

Cited by 5 publications

References 10 publications

Constituents and Pharmacological Activities of Myrcia (Myrtaceae): A Review of an Aromatic and Medicinal Group of Plants

Constituents and Pharmacological Activities of Myrcia (Myrtaceae): A Review of an Aromatic and Medicinal Group of Plants

Ecological and geographical variation of morphometric parameters and flavonoid composition of Rhododendron parvifolium

UHPLC-UV/PDA Method Validation for Simultaneous Quantification of Luteolin and Apigenin Derivatives from Elaeis guineensis Leaf Extracts: An Application for Antioxidant Herbal Preparation

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