Efeito do tratamento com própolis de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich em camundongos

Abstract

RESUMO:Neste trabalho foi investigado o efeito do extrato hidroalcoólico de própolis (EHP) de Scaptotrigona aff. postica sobre o desenvolvimento do tumor de Ehrlich na forma sólida, sobre a celularidade dos órgãos linfóides dos animais portadores de tumor, bem como, sobre a produção de óxido nítrico (NO) pelos macrófagos destes animais. Camundongos Swiss foram divididos em quatro grupos: controle, EHP 0,5; EHP 5 e EHP 50, os quais foram tratados por via intraperitoneal com dose única de solução salina (NaCl 0,9%); 0,5; 5 ou 50 mg de EHP/kg de animal, respectivamente. Depois de 48 h do tratamento, os animais foram inoculados com 10 5 células do tumor de Ehrlich nas patas. Os resultados mostraram que o tratamento com EHP nas doses de 5 e 50 mg/kg inibiu de forma significativa o desenvolvimento do tumor a partir do 6º dia pós-inóculo quando comparado ao controle e ao EHP 0,5. Além disso, houve aumento significativo da celularidade do baço e da medula óssea nos grupos EHP 0,5 e EHP 5 em relação ao controle. A produção de NO estimulada com concanavalina A (ConA) apresentou uma significante diminuição nos grupos tratados com EHP em relação ao controle. Pode-se concluir que o tratamento com EHP apresentou efeito antitumoral quando administrado nas doses de 5 e 50 mg/kg, o que pode estar relacionado com a sua composição química e com a inibição da produção de NO.Unitermos: própolis, Scaptotrigona aff. postica, tumor de Ehrlich, abelhas sem ferrão, Meliponinae.ABSTRACT: "Effect of propolis of Scaptotrigona aff. postica on the development of the tumor of Ehrlich in mice". It was investigated the effect of hydroalcoholic extract (HEP) of propolis from Scaptotrigona aff. postica on the solid Ehrlich tumor, on the tumor-bearing mice lymphoid organs and on the nitric oxide (NO) production. Swiss mice were divided in 4 groups: control, HEP 0.5; HEP 5 and HEP 50 that was treated by intraperitoneal route with a single dose of saline solution (NaCl 0.9%) or 0.5 or 5 or 50 mg of HEP/kg body weight, respectively. After 48 h of treatment, the animals were inoculated with 10 5 tumor cells in their footpad. The results showed that the treatment with HEP in the doses of 5 and 50 mg/kg inhibited the development of the tumor from the 6 th day post inoculums when compared to the control and to the HEP 0.5 groups. Besides, there was an increase of spleen and bone marrow cell number in HEP 0.5 and HEP 5 as compared to the control. Concanavalin A (ConA)-stimulated NO production was decreased in all HEP-treated groups when compared to the control. In conclusion, the treatment with HEP had an anti-tumor effect what may be related to its chemical composition and to the inhibition of NO production.

The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil

“…postica on Ehrlich tumor development in mice [8]. Propolis inhibited the tumor growth and increased the cell number in bone marrow and spleen.…”

Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propolis: a brief review

“…In fact, various biological properties have been demonstrated and attributed to different types of propolis, including antibacterial, antifungal, antiprotozoal, antioxidant, antitumor, anti-infl ammatory, anesthetic, wound healing, immunomodulatory, antiproliferative and anticariogenic activities (Dobrowolski et al, 1991;Ivanovska et al, 1995;Moura et al, 1999;Kujumgiev et al, 1999;Isla et al, 2001;Chen et al, 2004;Simões et al, 2004;Duran et al, 2006;Medic-Saric et al, 2009;Araujo et al, 2010;Sforcin, 2007;Libério et al, 2009;Pagliarone et al, 2009, Paulino et al, 2003.…”

Chemopreventive Effect of Brazilian Green Propolis on Experimental Dermal Carcinogenesis in Murine Model

“…One hypothesis to explain these results is that the hydroalcoholic extract of green propolis inhibits the synthesis of nitric oxide that facilitates the rapid growth tumor (Araújo et al, 2010). Another explanation is that caffeic acid phenethyl ester (CAPE), a derivative of caffeic acid, and another anticancer ingredient called ARC (artepillin C) inactivates PAK1 which is one of the responsibles for tumor growth (Orsolic et al, 2005;Messerli et al, 2009;Wu et al, 2011).…”