Comparative profiles of Achyrocline alata (Kunth) DC. and A. satureioides (Lam.) DC., Asteraceae, applying HPLC-DAD-MS

Grassi-Zampieron, Rafaela F.; França, Leonardo V.; Carollo, Carlos Alexandre; Vieira, Maria do Carmo; Oliveros‐Bastidas, Alberto; Siqueira, João Máximo de

doi:10.1590/s0102-695x2010000400017

Cited by 16 publications

(9 citation statements)

References 13 publications

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“…The concentration of kaempferol in F 1 was 6.4% ( w / w ), calculated using the ACB analytical curve. Grassi‐Zampieron et al . observed the presence of glycosylated kaempferol.…”

Section: Resultsmentioning

confidence: 99%

“…The concentration of kaempferol in F 1 was 6.4% (w/w), calculated using the ACB analytical curve. Grassi-Zampieron et al 21 common for this flavonoid class. 43,44 The structural elucidation of compounds 2, 3, and 4 was possible after isolation by HPCCC using UPLC-QTOF-HDMS (Table 3) and NMR (Table 4).…”

Section: Isolation Purification and Identification Of Phenolic Commentioning

confidence: 98%

“…More specifically, 3 O MQ is a methoxyflavonoid with pronounced biological activity, including anticancer, antioxidant, and antiviral action . Yet, the methods used to extract QCT, LUT, and 3 O MQ from A. satureioides , which include thin‐layer chromatography, column chromatography and liquid–liquid extraction, presented disadvantages such as long run times, complexity (too many steps), lower yield, and high costs …”

Section: Introductionmentioning

confidence: 99%

“…19 Yet, the methods used to extract QCT, LUT, and 3OMQ from A. satureioides, which include thin-layer chromatography, column chromatography and liquid-liquid extraction, presented disadvantages such as long run times, complexity (too many steps), lower yield, and high costs. 6,9,11,20,21 ACB, a bichalcone, has a unique chemical structure. In fact, chalcones are biosynthetic precursors of flavonoids, and their biological activities include antimalarial, 22 cytotoxic, anti-inflammatory, and anticancer properties.…”

Section: Introductionmentioning

confidence: 99%

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Semi‐preparative isolation and purification of phenolic compounds from Achyrocline satureioides (Lam) D.C. by high‐performance counter‐current chromatography

Bianchi

Kaiser

Pittol

et al. 2018

Phytochemical Analysis

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Introduction Phenolic compounds present in Achyrocline satureioides are known to have therapeutic benefits like antioxidant, anti‐inflammatory, and antitumour properties. The main polyphenols present in the plant are quercetin (QCT), luteolin (LUT), 3‐O‐methylquercetin (3OMQ), and achyrobichalcone (ACB). However, the effective isolation and purification of these compounds from A. satureioides inflorescences are not an easy task. Objective To develop an efficient high‐performance counter‐current chromatography (HPCCC) method for quick separation and purification of naturally occurring phenolic compounds from the extract of A. satureioides. Methodology A two‐step HPCCC semi‐preparative isolation method was developed using a solvent system composed of n‐hexane/ethyl acetate/methanol/water (0.8:1.0:0.8:1.0) and dichloromethane/methanol/water (3.5:3.5:2.5). Results The HPCCC method was used to obtain two fractions. The first fraction (F1) contained high levels of ACB, among other constituents, while the second fraction (F2) contained mostly QCT, LUT, and 3OMQ. Besides the high ACB content, F1 contained three other flavonoid‐aglycones (kaempferol, 97.3%; isokaempferide, 92.4%; and 3,3′‐di‐O‐methylquercetin, 95.2%) identified by an ultra‐performance liquid chromatography system coupled to a quadrupole time‐of‐flight with high‐definition mass spectrometry (UPLC‐QTOF/HDMS) and nuclear magnetic resonance (NMR) analysis. Purity levels of ACB, 3OMQ, QCT, and LUT were 98.0, 97.0, 97.5, and 90.2%, respectively. Conclusion This is the first time that high purity ACB and six other flavonoids were obtained from A. satureioides inflorescences by HPCCC. These excellent results reveal the potential and versatility of HPCCC as a technique to produce different types of products from this plant species on a semi‐preparative scale: enriched fractions, new metabolites, or high purity compounds.

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“…The concentration of kaempferol in F 1 was 6.4% ( w / w ), calculated using the ACB analytical curve. Grassi‐Zampieron et al . observed the presence of glycosylated kaempferol.…”

Section: Resultsmentioning

confidence: 99%

Section: Isolation Purification and Identification Of Phenolic Commentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Semi‐preparative isolation and purification of phenolic compounds from Achyrocline satureioides (Lam) D.C. by high‐performance counter‐current chromatography

Bianchi

Kaiser

Pittol

et al. 2018

Phytochemical Analysis

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“…Polar compounds between 11-17 min were also observed in the chromatogram. These compounds showed an UV spectrum characteristic of the caffeoyl/feruloyl group ( Grassi-Zampieron et al 2010 ) and a molecular weight range of 990-1638 Da ( Table I ). The fragmentation patterns of these peaks are similar to the sequential losses of caffeoyl acids and hexose moieties ( Fig.…”

Section: Resultsmentioning

confidence: 99%

In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii

Queiroz

Carollo

Toffoli-Kadri

et al. 2016

Mem. Inst. Oswaldo Cruz

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The polar hydroethanolic extract from Selaginella sellowii(SSPHE) has been previously proven active on intracellular amastigotes (in vitro test) and now was tested on hamsters infected with Leishmania (Leishmania) amazonensis (in vivo test). SSPHE suppressed a 100% of the parasite load in the infection site and draining lymph nodes at an intralesional dose of 50 mg/kg/day × 5, which was similar to the results observed in hamsters treated with N-methylglucamine antimonate (Sb) (28 mg/Kg/day × 5). When orally administered, SSPHE (50 mg/kg/day × 20) suppressed 99.2% of the parasite load in infected footpads, while Sb suppressed 98.5%. SSPHE also enhanced the release of nitric oxide through the intralesional route in comparison to Sb. The chemical fingerprint of SSPHE by high-performance liquid chromatography with diode-array detection and tandem mass spectrometry showed the presence of biflavonoids and high molecular weight phenylpropanoid glycosides. These compounds may have a synergistic action in vivo. Histopathological study revealed that the intralesional treatment with SSPHE induced an intense inflammatory infiltrate, composed mainly of mononuclear cells. The present findings reinforce the potential of this natural product as a source of future drug candidates for American cutaneous leishmaniasis.

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Cuyo

Álvarez¹

2019

Pharmacological Properties of Native Plants From Argentina

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Comparative profiles of Achyrocline alata (Kunth) DC. and A. satureioides (Lam.) DC., Asteraceae, applying HPLC-DAD-MS

Cited by 16 publications

References 13 publications

Semi‐preparative isolation and purification of phenolic compounds from Achyrocline satureioides (Lam) D.C. by high‐performance counter‐current chromatography

Semi‐preparative isolation and purification of phenolic compounds from Achyrocline satureioides (Lam) D.C. by high‐performance counter‐current chromatography

In vivo antileishmanial activity and chemical profile of polar extract from Selaginella sellowii

Cuyo

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