Anti-inflammatory and analgesic potential of hydrolyzed extract of Agave sisalana Perrine ex Engelm., Asparagaceae

Abstract

RESUMO: "Potencial anti-inflamatório e analgésico do extrato hidrolisado deUnitermos: Agave sisalana, Asparagaceae, Agavoideae, hemolítica anti-inflamatória, analgesia. ABSTRACT:The hemolytic, anti-inflammatory and antinociceptive properties from hydrolyzed extract Agave sisalana Perrine ex Engelm., Asparagaceae (HEAS) was evaluated on classic inflammation models. Male Swiss mice and male Wistars rats received HEAS (500 mg/kg) in two administration p.o. and i.p. in saline solution 0.9%. The acid hydrolysis inhibited the hemolytic action of saponins due to the retreat of side chain sugar. The treatment of the ear induced oedema by xylene with HEAS significantly reduced in two routes 13±1.5 and 10±0.63 mg, respectively, p.o. and i.p., in comparison with controls 27±1.5 saline and 13.5±1.2 AAS. The HEAS also diminished edema induced by carrageenin 43±1.58 mg (p.o.) and 17±1.26 mg (i.p.), when compared with control groups 52±1.58 mg (saline) and 10.05±1.58 (indomethacin). HEAS showed analgesic effects in abdominal constrictions 30.7% (p.o.), 88.7% (i.p.) comparable to that produced by (AAS) 70.6%. However in granuloma cotton pellet a chronic model of inflammation just the i.p. pathway decreased granulomatous tissue (20.4±1.32 mg) compared with controls 30.5±2.53 mg (saline) and 20.2±2.18 mg (dexamethasone). These data suggest that HEAS has anti-inflammatory and analgesic activity on acute and chronic processes.

In vitro ovicidal and larvicidal activity of Agave sisalana Perr. (sisal) on gastrointestinal nematodes of goats

“…Previous studies reported that A. sisalana had several biological effects, including antimicrobial (Santos et al, 2009), anti-inflammatory (Dunder et al, 2010) and anthelmintic properties (Domingues et al, 2010). Steroidal saponins (Ding et al, 1989;Zou et al, 2006;Chen et al, 2011) and flavonoids (Chen et al, 2009) are among the secondary metabolites that have been isolated from this plant.…”

Applications of the hexanic fraction of Agave sisalana Perrine ex Engelm (Asparagaceae): control of inflammation and pain screening

“…HFAS maintained the reduction of oedema during the second phase, which involves PGE 2 and leukocyte infiltration. Another study (Dunder et al 2010) showed that hydrolysed extracts of A. sisalana inhibited oedema in the same model, but at a higher dose. In the present study, with a more purified compound that is richer in steroidal sapogenins, more promising results were obtained with lower doses.…”

“…Several studies have shown different activities of extracts derived from the crude juice of A. sisalana. The crude juice exhibits bactericidal, antihelmintic and anti-inflammatory Financial support: FAPESP, CAPESactivity (Santos et al 2009, Botura et al 2010, Dunder et al 2010). In the Dominican Republic, a traditional medicine called Agave intermixta Trel is widely used in the treatment of arthritis and infections (Garcia et al 1999).…”

Biological activities of Agave by‐products and their possible applications in food and pharmaceuticals

“…Moreover, Da Silva et al 103 demonstrated that the application of A. attenuata saponin (100 μg kg −1 ) to vascular permeability inflammation reduced inflammation by 60% compared to the control. Dunder et al 104 induced inflammation in mouse ears and legs and found that A. sisalana extract at 500 mg kg −1 was able to decrease the inflammation. Compared to a control, ear inflammation was reduced by 50-60%, and leg inflammation was decreased by 10-60%.…”