Four Brazilian Maytenus salicifolia Reissek (Celastraceae) groups studied by TLC and UV/Vis spectrophotometry

Abstract

RESUMO: "Estudo de quatro populações de Maytenus salicifolia Reissek (Celastraceae) por CCD e espectrometria na região do UV/Vis". A grande variedade de angiospermas apontou a necessidade do desenvolvimento de sistemas de classificação botânica apoiada pela fitoquímica, bioquímica e outras. Recentemente, técnicas de análise utilizadas para o isolamento e caracterização de metabólitos secundários vêm sendo empregadas como métodos auxiliares rápidos e eficientes para identificação e classificação de espécies vegetais. M. salicifolia é popularmente conhecida no Brasil, como "cafezinho". O chá obtido a partir de folhas frescas é usado topicamente para aliviar pruridos e sintomas alergiformes. Este trabalho apresenta a utilização do CCD em sílica gel e espectrofotometria no UV / Vis como métodos auxiliares na identificação botânica de M. salicifolia. Os resultados demonstraram que este processo pode ser usado na diferenciação de plantas do mesmo gênero, assim como detectar variações químicas entre indivíduos de uma mesma espécie. Unitermos:Maytenus salicifolia, Celastraceae, triterpenos pentacíclicos, UV/Vis, CCD. ABSTRACT:The great variety of angiosperms shows the need to development of botanical classification systems supported by phytochemistry, biochemistry and others. Recently, techniques of analysis used for the isolation and characterization of secondary metabolites have been employed as auxiliary quick and efficient methods for the identification and classification of plant species. M. salicifolia is popularly known in Brazil, as "small coffee" and decoct obtained from its fresh leaves is topically used to alleviate itches and other skins allergic symptoms. This work presents the use of TLC and UV/Vis spectrophotomety processes to be applied like an auxiliary method in botanical taxonomy. The results demonstrate that this process can be used in differentiation of the same genera species, and in the selection of chemical variations between individuals of the same species.

Maytenus salicifolia Reissek, Celastraceae: triterpenes isolated from stems and antioxidant property of extracts from aerial parts

“…These compounds gave a Liebermann-Buchard test positive for triterpenes (Valladão et al, 2009). The structural elucidation of the PCTT 1 to 6 was based on its 1 H and 13 C NMR spectral data, including for compound 1, the 2D (HSQC, HMBC and NOESY) data.…”

“…Triterpene 3 was identified as friedelin, Maytenus salicifolia: triterpenes isolated from stems and antioxidant property of extracts from aerial parts Cássia G. Magalhães et al which was previously isolated from hexane extract of the leaves of M. salicifolia (Miranda et al, 2006). Friedelin and 3β-friedelinol has been used in quality control of species of the genera Maytenus, particularly of Maytenus ilicifolia (Valladão et al, 2009). The 13 C NMR spectrum of triterpene 4 (25.0 mg) showed signal at δC 109.68 and δC 150.47, attributed to olefinic carbon, and at δC 78.97 associated to carbinolic carbon (C-3).…”

“…The tea (decoct) obtained from the fresh leaves is topically used to alleviate itches and symptoms of skin allergies (Valladão et al, 2009).…”