Cytotoxic activity of the sub-fraction 2125 from Vernonia scorpioides against Sarcoma 180 tumor cells in mice

Abstract

RESUMO: "Ação citotóxica da sub-fração 2125 de Vernonia scorpioides contra células do Sarcoma 180 em camundongos". O efeito da sub-fração SF-2125 obtida do extrato das folhas de Vernonia scorpioides foi investigado em camundongos portadores do tumor ascítico Sarcoma 180 (S180). Os animais foram tratados com SF-2125 na concentração de 5 mg/kg, administrada por vias intraperitoneal e intravenosa durante o desenvolvimento do tumor. O tratamento com SF-2125 5 mg/kg i.p. aumentou o tempo de vida dos animais, manteve seu peso corporal e o tumor na forma ascítica não teve desenvolvimento. O tratamento intravenoso não reduziu o volume do tumor. Unitermos: Sarcoma 180, sub-fração 2125, Vernonia scorpioides.ABSTRACT: The effect of the selected sub-fraction SF-2125 of the Vernonia scorpioides leaf extract on Sarcoma 180 (S180) ascitic tumor-bearing mice was investigated. The animals were treated with SF-2125 at a concentration of 5 mg/kg, administered intraperitoneally and intravenous during the development of the tumor. Treatment with SF-2125 5 mg/kg i.p. increased the lifespan of the animals, maintained their body and the ascitic tumor showed no development. Intravenous treatment did not reduce the tumor volume.

Genus Vernonia (Asteraceae): A Promising Source of Antitumor Agents with Pharmacological Potentials

“…The antitumor activity study was carried out in mice with Ehrlich ascitic and solid tumors where the dichloromethane sub fraction totally reduced the ascites volume and the number of tumor cells in the peritoneum of the mice at 5 mg/kg body weight" [46]. "The treatment with ethyl acetate sub-fraction extracted from the dichloromethane sub-fraction eliminated sarcoma 180 ascitic tumor in mice" [69].…”

Effects of the topical application of an ethyl acetate fraction from Vernonia scorpioides on excisional wounds infected with Staphylococcus aureus in rats

“…Previous studies of V. scorpioides crude extract and its derived chloroform and hexane fractions have shown fungicide and moderate bactericide activity (Freire et al, 1996), and its use as a treatment for mange (scabies) has been recorded (Lans et al, 2001). In mice, the dichloromethane fraction (5 mg/kg) from the crude extract totally inhibited tumor development (Pagno et al, 2006), while subfraction 2125 from the crude extract (5mg/kg) inhibited tumor growth in the Sarcoma 180 ascitic tumor model in mice (Kreuger et al, 2009). The topical use of its alcoholic extract (200 mg of the V. scorpioides hydrogel) showed mild wound healing effects in rats when the wounds were treated topically for 30 days (Leite et al, 2002), although noninfected skin ulcers treated with an ointment 20% of the crude concentrated extract from V. scorpioides with solid Vaseline and lanoline (1:2) showed toxicity for the tissues when applied immediately after the incision in mice.…”