Anti-mitotic activity towards sea urchin eggs of dichloromethane fraction obtained from Allamanda schottii Pohl (Apocynaceae)

Abstract

RESUMO: "Atividade anti-mitótica da fração diclorometano obtida de Allamanda schottii Pohl (Apocynaceae) sobre ovos do ouriço do mar." Allamanda (Apocynacea) é um gênero de arbustos escandentes conhecido por produzir compostos com várias atividades biológicas. Trabalhos anteriores têm mostrado um efeito anti-proliferativo do extrato etanólico de Allamanda schottii sobre células leucêmicas. O presente trabalho foi realizado para avaliar o efeito da fração diclorometano, obtida de Allamanda schotti, sobre os ovos de ouriço-do-mar de Echinometra lucunter, como um modelo multicelular para estudar atividade anti-tumoral. Nossos resultados mostram uma inibição do desenvolvimento dos ovos de uma maneira dose-dependente na presença da fração diclorometano. Os valores de IC 50 para a primeira e terceira clivagem e para o estágio de blástula foram de 103,7 µg/mL, 33.1 µg/mL e 10,2 µg/mL, respectivamente. Estes resultados também demonstram um efeito acumulativo da fração sobre os embriões do ouriço-do-mar. No presente trabalho, esta expressiva atividade anti-mitótica da fração diclorometano sobre o desenvolvimento embrionário do ouriço-do-mar, um modelo multicelular, reforça o potencial antitumoral de Allamanda schotti. Unitermos: Allamanda schottii, ouriço do mar, atividade anti-mitótica, anti-tumoral.ABSTRACT: Allamanda (Apocynaceae) is a genus of climbing shrubs known for producing compounds with a range of biological activities. Previous works have shown the anti-proliferative effect of the ethanolic extract of Allamanda schottii on leukemic cells. The present work was conducted to evaluate the effects of dichloromethane fraction, obtained from Allamanda schottii, on sea urchin Echinometra lucunter eggs, as a multicellular model for evaluating anti-tumor activity. Our results show an inhibition of sea urchin development in a dose-dependent manner in the presence of dichloromethane fraction. The IC 50 values for first and third cleavage and blastulae stage were 103.7 µg/mL, 33.1 µg/mL and 10.2 µg/mL, respectively. These results also demonstrate the cumulative effect of this fraction on sea urchin embryos. In the present work, the expressive anti-mitotic activity of dichloromethane fraction towards sea urchin eggs, a multicellular model, reinforces the anti-tumor potential of the Allamanda schotti.

Cytotoxicity and genotoxicity of Allamanda and Plumeria species

“…Some Allamanda species contain glabridin, new lignin, kaempferol, naringenin, allamandicin 1 , squalene, α-tocopherol, vitamin E, and 9,12,15octadecatrien-1-ol 2 . They showed algicidal properties 3 , anti-proliferative effect against leukaemic and endothelial cells 4 , anti-mitotic activity towards sea urchin eggs, reinforcing the anti-tumour potential 5 , and tyrosinase inhibitory activity 1 .…”

“…Additionally, Allamanda and Plumeria species have been used for the treatment of many diseases such as rheumatism, diarrhoea, blennorrhea, venereal disease, leprosy, psychosis, diuresis, laxative, and inducing vomiting [6][7][8] . Although some poisonous have been reported, such as when used in excess, it becomes a strong laxative and causes excessive vomiting and sometimes death 8 , algicidal poisons 3 , strong cytotoxic effects on leukaemic and endothelial cells 4 , and anti-mitotic activity towards sea urchin eggs 5 . The symptoms of poisoning include a slow and irregular pulse, which can be followed by vomiting, and finally, toxic shock.…”

Effects of the green alga, Bornetella oligospora (SolmsLaubach), metabolite fractions on the cleavage and freeswimming hatching stages of sea urchin embryos

“…Sea urchins, Tripneustes gratilla (Linnaeus, 1758), were collected from the coast of Canday-ong, Dumaguete City, Philippines. Spawning was induced in the lab by intracoelomic injection of 0.5 M potassium chloride (KCl) [5]. Gametes were collected from each urchin in separate labelled beakers.…”

<i>Allamanda schottii</i> (Apocynaceae): nueva cita para la Flora Argentina

“…En Brasil, las hojas de A. schottii se emplean en medicina popular, como las de A. cathartica, como remedio purgante (Beringer, 1895). Se ha evaluado su efecto antitumoral (Anderson & al., 1988;Navarro Schmidt & al., 2006;Sousa & al., 2009), y su actividad antiviral (Müller & al., 2007).…”