Antimicrobial screening of some medicinal plants from Mato Grosso Cerrado

Silva, Iberê E; Filho, Valdir Cechinel; Zacchino, Susana; Lima, Joaquim Corsino da Silva; Martins, Domingos Tabajara de Oliveira

doi:10.1590/s0102-695x2009000200011

Cited by 39 publications

(10 citation statements)

References 19 publications

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“…In a preliminary screening for its antimicrobial potential, it showed a broad spectrum potency, in line with previous studies on medicinal plants (Omoregie and Folashad 2013; Silva junior et al 2009; Zampini et al 2009). It is well known that Pseudomonas aeruginosa is a prototypical “multidrug resistant (MDR) pathogen” that is recognized for its ubiquity, intrinsically advanced antibiotic resistance mechanisms and association with serious illnesses.…”

Section: Discussionsupporting

confidence: 86%

In vitro antimicrobial potential of extracts and phytoconstituents from Gymnema sylvestre R.Br. leaves and their biosafety evaluation

Arora

Sood

2017

AMB Expr

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The in vitro antimicrobial screening of Gymnema sylvestre leaves against 13 test pathogens established its broad spectrum activity with average inhibition zone ranging from 14 to 23 mm. The antimicrobial activity of the classically- optimized aqueous extract was enhanced up to 1.45 folds, when subjected to statistical optimization using Response Surface Methodology (RSM) and was thermostable. Ethyl acetate was found to be the best organic extractant with Klebsiella pneumoniae 1 (31.5 mm) and Staphylococcus epidermidis (25.5 mm) being the most sensitive among Gram negative and Gram positive bacteria, respectively. Among the major group of phytoconstituents detected, tannins were the most abundant followed by flavonoids and phytosterols, while triterpenes were absent. Flavonoids and cardiac glycosides exhibited a broad range of antimicrobial potential, with inhibition zone ranging from 13 to 35 mm, where Candida albicans was the most sensitive organism. Ethyl acetate extract showed better potency with lowest Minimum inhibitory concentration (0.1–1 mg ml−1) than the aqueous extract (1–3 mg ml−1) and all partially purified phytoconstituents (0.1–10 mg ml−1). The ethyl acetate extract and flavonoids were highly potent, as they exhibited a total activity potency ranging from 41.4 to 1045 ml g−1. Time kill studies revealed their microbicidal action, where ethyl acetate extract had a kill time from 0 to 12 h. However, among phytoconstituents, flavonoids were the most effective (0–8 h). The MIC and time kill study was also compared to that of standard antibiotics. These findings indicate that Gymnema sylvestre can be a potential source for development of leading metabolites against pathogens of clinical importance like Pseudomonas aeruginosa, Candida albicans, Escherichia coli, Staphylococcus aureus etc. They were neither mutagenic nor cytotoxic, as revealed by Ames and MTT assay.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0416-z) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 86%

In vitro antimicrobial potential of extracts and phytoconstituents from Gymnema sylvestre R.Br. leaves and their biosafety evaluation

Arora

Sood

2017

AMB Expr

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“…Desse modo, o estudo do processo germinativo de espécies como Bowdichia virgilioides se faz necessário. Esta Fabaceae, encontrada em diversos tipos de vegetação da Mata Atlântica, Caatinga, Pantanal e Cerrado, possui comportamento de espécie colonizadora e pode ser utilizada na recuperação de áreas degradadas (CARVALHO, 2006), além de apresentar grande potencial fitoterápico (SILVA JÚNIOR et al, 2009;THOMAZZI et al, 2010).…”

Section: Introductionunclassified

A amplitude amostral interfere nas medidas de germinação de <i>Bowdichia virgilioides</i> Kunth?

Ribeiro‐Oliveira

Ranal²,

Santana³

2013

Ciênc. Florest.

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RESUMOÉ comum encontrar na literatura trabalhos sobre germinação de sementes nativas de uma mesma espécie com tamanhos de amostra diferenciados. No entanto, não se sabe quais são as consequências disso para os resultados obtidos. Assim, objetivou-se estudar o comportamento das medidas de germinação frente à variação do número de sementes que compõe a amostra para Bowdichia virgilioides. O experimento foi conduzido em câmara de germinação, sob luz branca fluorescente contínua, a 26,2 ± 2,5 ºC. O delineamento utilizado foi o inteiramente casualizado, em esquema fatorial 3 x 3 (três tamanhos de amostra x três lotes), com número de repetições fixo e igual a quatro. A semeadura foi realizada em caixas tipo gerbox, contendo 25, 50 ou 100 sementes, perfazendo 100, 200 ou 400 sementes por amostra. A germinabilidade, tempo inicial, médio e final, velocidade média e VE (IVG), coeficiente de variação do tempo, incerteza e sincronia da germinação foram avaliados, sendo consideradas germinadas as sementes com protrusão de embrião. Gráficos de frequência relativa também foram construídos. Dentre estas medidas, as mais frágeis ao incremento do tamanho da amostra foram o VE e a incerteza. O VE, além de ser influenciado pelo tamanho da amostra, também foi influenciado pelo contrabalancear entre germinabilidade e velocidade média, demonstrando ser uma medida imprópria para mensurar velocidade de germinação quando a capacidade germinativa dos lotes for diferente. As medidas de tempo, uniformidade, sincronia, velocidade média e a germinabilidade não tiveram seus resultados afetados pelos diferentes tamanhos da amostra. A germinabilidade, entretanto, mostrou-se a medida mais estável do processo germinativo, não sendo influenciada pelo tamanho da amostra, mesmo quando analisada por meio de lotes com qualidades muito discrepantes. Palavras-chave: teste de germinação; qualidade fisiológica; sucupira-preta; tamanho ótimo de amostra. ABSTRACTIt is usual to find papers about seed germination of native species using different sample sizes. However, the consequences of these procedures on the results are unknown. This study measured the germination process of Bowdichia virgilioides based on different seed sample sizes. The assay was conducted in a germination chamber under continuous fluorescent white lamps and 26.2 ± 2.5 ºC. The design was completely randomized, as a 3 x 3 factorial (three sample sizes x three seed lots), with four replications per treatment. The sowing was done in gerbox, each one containing 25 seeds, making up 100, 200 and 400 seeds per sample analyzed. Germinability, time (first, average and final), speed (mean germination rate and Maguire's index -VE or IVG), coefficient of variation of the germination time, uncertainty and synchrony of the germination process were evaluated. Those seeds with embryo protrusion were considered germinated. Germination relative frequency graphics were also made. Among these measurements, the most fragile to

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“…The majority of these plants used in this study are applied in traditional medicine. Recent studies (6)(7)(8)(9)(10) have revealed that medicinal plants from various parts of the world can provide a rich source of antibacterial activities.…”

Section: Results and Discussion:-mentioning

confidence: 99%

“…After that, they have been extracted with 150 mL of (hot water, cold water, ethanol, methanol, hexane, petroleum ether and acetone respectively (5)(6)(7)(8) . Extracts were filtered through Whatman No.…”

Section: Materials and Methods:-mentioning

confidence: 99%

The Antimicrobial Activities of Some Plants Extracts Against Candida Albicans Ssp. And Staphylococccus Aureus Ssp.

Zahrani¹,

Refaei²,

Marwani³

et al. 2016

IJAR

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This study was conducted to determine the effect of hot and cold water, Ethanol, Methanol, Acetone, Petroleum Ether and Hexane extracts of Allium Sativum, ZingiberOfficinaleand Nigella sativaagainst Staphylococcus aureusssp. and Candida albicans ssp. after 24 or 48 hrs of incubation where the inhibition zones on Nutrient Agar and Potato Dextrose agar appeared. The results showed that the hot water extract was not effective at all compared other extracts. Also, results indicated that both Candida albicans ssp. was more sensitive toward all plants extracts except water extracts compared with Staphylococcus aureusssp. which was higher resistance toward almost extracts.

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Antimicrobial screening of some medicinal plants from Mato Grosso Cerrado

Cited by 39 publications

References 19 publications

In vitro antimicrobial potential of extracts and phytoconstituents from Gymnema sylvestre R.Br. leaves and their biosafety evaluation

In vitro antimicrobial potential of extracts and phytoconstituents from Gymnema sylvestre R.Br. leaves and their biosafety evaluation

A amplitude amostral interfere nas medidas de germinação de <i>Bowdichia virgilioides</i> Kunth?

The Antimicrobial Activities of Some Plants Extracts Against Candida Albicans Ssp. And Staphylococccus Aureus Ssp.

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