Osmotic and morphological effects on red blood cell membrane: action of an aqueous extract of Lantana camara

Maiworm, Adalgisa Ieda; Presta, G.; Santos-Filho, Sebastião David; Paoli, Severo de; Giani, Tania Santos; Fonseca, A S; Bernardo-Filho, Mário

doi:10.1590/s0102-695x2008000100010

Cited by 7 publications

(6 citation statements)

References 26 publications

(24 reference statements)

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“…Blood smears were prepared, dried, fixed and stained by May-Grunwald-Giemsa method (Junqueira and Carneiro 2004;Maiworm et al 2008). After that, the blood smears were scanned under 1009 of light microscope (Olympus CX21FSI, Japan) and images of red blood cells were taken by using an AmScope MU Series digital camera.…”

Section: Evaluation Of Membrane Damage Using Rbcsmentioning

confidence: 99%

Cytotoxic and apoptotic activities of extract of Amaranthus spinosus L. in Allium cepa and human erythrocytes

Prajitha

Thoppil

2016

Cytotechnology

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The present study examined the apoptosis inducing effects of Amaranthus spinosus L. aqueous extract in Allium cepa root meristematic cells and human erythrocytes. Cytogenetic assay revealed many apoptosis inducing cytogenetic aberrations viz., cytoplasmic breakage, cytoplasmic disintegration, cytoplasmic shrinkage, receding of cytoplasm, cytoplasmic vacuolation, enucleated cell, ghost cell, nuclear vacuolation, nuclear fragmentation and nuclear disintegration. A remarkable modification of red blood cell surface morphology was observed in the result of RBC assay. The treated RBCs show membrane blebbing and shrinkage, features typical for apoptosis in nucleated cells. Significant induction of cell death was observed in treated Allium root tip cells after Evans blue staining, disclosing the membrane damage potential of the plant extract. TTC assay results in reduced mitochondrial/ metabolic activity in Allium root tip cells after treatment, designating the adverse effect of plant extract on mitochondrial respiratory chain. These results confirm the apoptosis inducing potential of A. spinosus extract. Confirming the present results by further in vitro studies, it can be effectively targeted against cell proliferation during cancer treatment by inducing apoptosis. Thus from the present investigation it can be concluded that the aqueous extract of A. spinosus exhibited apoptosis induction and cytotoxic activities.

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Section: Evaluation Of Membrane Damage Using Rbcsmentioning

confidence: 99%

Cytotoxic and apoptotic activities of extract of Amaranthus spinosus L. in Allium cepa and human erythrocytes

Prajitha

Thoppil

2016

Cytotechnology

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“…In the show debate, we discovered that molasses, white peel, and red peel have improved Osmotic fragility. Ours develops through mutual understanding with (16,17). The osmotic delicacy measurement used in this study by (17) revealed that Lantana camara extract appears to alter the lm's acuity at NaCl concentrations close to physiologic levels.…”

Section: Discussionmentioning

confidence: 73%

Pomegranate (Molasses, White Peel, Red Peel) effect in reducing Phenylhydrazine-induced Spleen injury and hemolytic anemia incidence

Soliman

Mansour

Ammar

et al. 2023

Preprint

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In this study, the effects of pomegranate molasses, white peel extract, and red peel extract on male rats with phenylhydrazine -induced anaemia are discussed. Reduced body weight, haemoglobin concentration, hematocrit, red cell count, and red blood cell survival were the results of phenylhydrazine-induced anaemia. Adult haemoglobin type A, MPV, basophils, PDW, normal adult haemoglobin type A2, eosinophils, PDW, and PCT. Initial hemolysis due to fragility, complete hemolysis due to osmotic fragility, Adult haemoglobin type A, normal adult hemoglobin , MCHC, RDW-CV, PCT, and PDW, as well as eosinophils. In the histopathology analysis of the spleen, the PHZ exposed group had a higher incidence of macrophages carrying cellular debris as a result of degeneration inside the white pulp and red pulp with edematous gaps. Additionally, PHZ provided a magnified view of the final field that demonstrated the presence of macrophages inside the white pulp along with vacuolization and necrotic regions. A magnified view of the last field inside the red pulp is also shown, revealing a significant amount of necrotic cells, bleeding, and sporadic apoptotic cells. There was an increase Body Weight, G6PD, Hemoglobin Concentration, Heamatocrit, Red Cell Count, Red Blood Cell Survival, PDW, Normal Adult Hemoglobin Hb A2, MPV, MCHC, Basophils, Hemoglobin Adult Hb A, RDW, PCT, Eosinophils. The splenic parenchyma in the red and white pulps of PHZ-exposed rats receiving molasses pomegranate treatment showed a lesser degree of recovery and the presence of apoptotic cells along with bleeding and vacuolization. Strong antioxidants in molasses, white peel, and red peel of pomegranates preserve haematological and spleen tissue.

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“…The cell stiffness increased firstly and then decreased according to the qualitative analysis of the approaching branch of force-distance curves, but the cells treated with AM for 48 h were stiffer than both the normal cells and the cells treated with AM for 72 h. Stiffness of cells is a parameter of increasing importance in cellular physiology. On the one hand, cells mainly rely on cytoskeleton and osmotic pressure to maintain the basic morphology and function [ 36 - 39 ]. On the other hand, a filamentous cytoskeleton and osmotic pressure collectively govern the physical shape and mechanical properties (viscoelastic properties and stiffness) of eukaryotic cells [ 40 - 42 ].…”

Section: Resultsmentioning

confidence: 99%

The influence of aminophylline on the nanostructure and nanomechanics of T lymphocytes: an AFM study

Huang

Zhou

2014

Nanoscale Res Lett

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Although much progress has been made in the illustration of the mechanism of aminophylline (AM) treating asthma, there is no data about its effect on the nanostructure and nanomechanics of T lymphocytes. Here, we presented atomic force spectroscopy (AFM)-based investigations at the nanoscale level to address the above fundamental biophysical questions. As increasing AM treatment time, T lymphocytes' volume nearly double increased and then decreased. The changes of nanostructural features of the cell membrane, i.e., mean height of particles, root-mean-square roughness (Rq), crack and fragment appearance, increased with AM treatment time. T lymphocytes were completely destroyed with 96-h treatment, and they existed in the form of small fragments. Analysis of force-distance curves showed that the adhesion force of cell surface decreased significantly with the increase of AM treatment time, while the cell stiffness increased firstly and then decreased. These changes were closely correlated to the characteristics and process of cell oncosis. In total, these quantitative and qualitative changes of T lymphocytes' structure and nanomechanical properties suggested that AM could induce T lymphocyte oncosis to exert anti-inflammatory effects for treating asthma. These findings provide new insights into the T lymphocyte oncosis and the anti-inflammatory mechanism and immune regulation actions of AM.

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Osmotic and morphological effects on red blood cell membrane: action of an aqueous extract of Lantana camara

Cited by 7 publications

References 26 publications

Cytotoxic and apoptotic activities of extract of Amaranthus spinosus L. in Allium cepa and human erythrocytes

Cytotoxic and apoptotic activities of extract of Amaranthus spinosus L. in Allium cepa and human erythrocytes

Pomegranate (Molasses, White Peel, Red Peel) effect in reducing Phenylhydrazine-induced Spleen injury and hemolytic anemia incidence

The influence of aminophylline on the nanostructure and nanomechanics of T lymphocytes: an AFM study

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