Avaliação do potencial antimicrobiano de Plinia glomerata (Myrtaceae)

Abstract

RESUMO:A planta Plinia glomerata (Myrtaceae), popularmente conhecida como cabeludinha ou "jabuticaba-amarela", ocorre amplamente no sul do Brasil e é cultivada como ornamental e frutífera comestível. O presente trabalho avaliou as propriedades antimicrobianas dos extratos, frações e substâncias puras isoladas da planta contra bactérias e fungos patogênicos. As concentrações inibitórias mínimas (CIM) foram determinadas através do método de diluição em ágar. As frações acetato de etila (AE) e fração aquosa (AQ) demonstraram a melhor atividade contra Staphylococcus aureus. A fração AQ também foi efetiva contra Escherichia coli. As substâncias puras, ácido 3,4,3'-trimetóxi-fl avelágico-4'-O-glicosídeo e ácido 3,4,3'-trimetóxi-fl avelágico foram inativas até a concentração de 500 g/mL contra os microrganismos testados. Em relação aos resultados antifúngicos, os extratos metanólico (EM) e acetônico (EA) e a fração aquosa (AQ) mostraram boa atividade somente contra dermatófi tos. Os resultados obtidos permitem concluir que a Plinia glomerata possui princípios ativos com ação antimicrobiana, sugerindo que outras substâncias da planta estão agindo contra os microrganismos indicados ou a existência de efeitos sinérgicos.Unitermos: Plinia glomerata, atividade antimicrobiana, ácido 3,4,3'-trimetóxi-fl avelágico-4'-O-glicosídeo, ácido 3,4,3'-trimetóxi-fl avelágico.ABSTRACT: "Evaluation of the antimicrobial effects of Plinia glomerata (Myrtaceae)". Plinia glomerata, commonly known as "yellow jaboticaba" or "cabelluda" occurs widely in south of Brazil. This work evaluated the antimicrobial properties of the extracts, fractions and isolated compounds of this plant against pathogenic microorganisms, bacteria and fungi. The minimal inhibitory concentration (MIC) was determined through the method of dilution in agar. The ethyl acetate (EtOAc) and aqueous fractions demonstrated the best activity against Staphylococcus aureus. The aqueous fraction also exhibited good activity against Escherichia coli. The pure substances 3,4,3'-trimethoxy-fl avellagic-4'-O-glucose acid and 3,4,3'-trimethoxy-fl avellagic acid were inactive up to 500 g/mL. Regarding results against fungi, EA and EM extracts as well as AQ fraction showed good activity only against dermatophytes. The results suggest that P. glomerata produces active principles with antimicrobial activity, suggesting that other substances from the plant are acting against the microorganisms or the existence of synergic effects.

In vitro anti-staphylococcal activity of Hyptis martiusii Benth against methicillin-resistant Staphylococcus aureus: MRSA strains

“…For patients, the antimicrobial resistance increases the morbidity and mortality, whilst for healthcare institutions it means increasing costs (Dancer, 2001;Coutinho et al, 2005). Regarding the increasing clinical importance given to nosocomial and community bacterial infections and the progressive development of antimicrobial resistance, a great number of scientifi c researches emphasizing the antibacterial properties of plant products has been carried out (Hernández et al, 2003;Silva-Santos et al, 2004;Duarte et al, 2005;Gayoso et al, 2005;Michelin et al, 2005;Lima et al, 2006a,b;Santos et al, 2007;Serafi n et al, 2007;Silva et al, 2007;Aguiar et al, 2008;Silva et al, 2008;Salvagnini et al, 2008;Simões et al, 2008). Filtrates, infusions, macerated, juices, extracts and cataplasms from plants with medicinal properties have been applied in the treatment of various diseases since antiquity (Annuk et al, 1999;Hernández et al, 2003).…”

Antimicrobial Activity and Rates of Tannins in <i>Stryphnodendron adstringens</i> Mart. Accessions Collected in the Brazilian Cerrado

“…[17] screening Plinia glomerata, a plant species as rich in phenolic compounds as S. adstringens, reported that P. glomerata crude extract showed more significant anti- fungal activity than the purified fractions or the pure compounds isolated from that plant. With the exception of the accessions collected in Delfinópolis every other presented different MIC values for each individual and dissimilarities on tannin concentration were observed inter and intra plant populations.…”

Chemical and Antidiarrheal Studies ofPlinia cauliflora

“…These results are consistent with some published data on the chemical composition of plants belonging to the Myrtaceae family. For example, aerial parts of Plinia glomerata exhibited the presence of ellagic acid derivatives, 30 whereas Plinia pinnata showed flavonoid derivatives, 31 and the methanolic extracts of many fruits of the Myrtaceae family have already been studied for their hydrolyzable tannin and flavonoid content. 32 Quantification of chemical compounds indicated that both extracts have phenolic compounds, mainly flavonoids, and tannins that were present in higher concentrations in leaf extract than in fruit extract.…”