Concomitant chemopreventive and antibacterial effects of some Iranian plants from the genus Cousinia (Asteraceae)

Abstract

RESUMO: "Efeitos quimiopreventivo e antibacteriano concomintantes de algumas plantas iranianas do gênero Cousinia (Asteraceae)". Durante os últimos anos, várias espécies de Cousinia (Asteraceae) têm sido identifi cadas no Irã. No entanto, dados acerca de suas atividades biológicas permanecem limitados. O principal propósito desta pesquisa foi avaliar a potencial citotoxidade e os efeitos inibitórios de metaloproteinases da matriz (MMP) de sete extratos etanólicos de Cousinia utilizando um modelo de linhagem celular (Fibrosarcoma-WEHI 164). Além disso, investigamos a atividade antibacteriana destes extratos etanólicos de Cousinia, utilizando o método de difusão em disco. Dentre os extratos etanólicos, o extrato total de C. sulabadensis promoveu inibição signifi cativa da atividade de MMP de maneira dose-resposta (49,2 ± 0,51, p < 0,05). Todavia, este extrato exibiu o menor efeito de citotoxicidade em todas as concentrações testadas. A concentração necessária para produzir uma taxa de 50% de morte celular (IC50) com C. shulabadensis foi 304,5 ± 0,61 μg/mL. A IC50 calculada para a citotoxicidade dos outros extratos de espécies de Cousinia situou-se entre 18,4 ± 0,59 e 87,9 ± 0,58 μg/mL. A maior atividade antibacteriana foi observada para o extrato total de Cousinia phyllocephala. Em conclusão, este estudo corrobora que espécies de Cousinia mostram uma notável inibição da atividade de metaloproteinases da matriz. Os concomitantes efeitos inibitórios de MMP e baixa citotoxicidade observados em C. sulabadensis devem direcionar este extrato para futuros estudos de potenciais fi toterápicos anti-invasivos.Unitermos: Cousinia, citotoxicidade, IC50, zimografi a, antibacteriano, linhagem celular WEHI 164.ABSTRACT: During the past several years, various species of Cousinia (Asteraceae) have been authenticated in Iran. However, data concerning their biological activities remain limited. The main purpose of this research was to assess potential cytotoxicity and matrix metalloproteinases (MMP) inhibitory effects of seven ethanol extracts of Cousinia using a cell line model (Fibrosarcoma-WEHI 164). We further investigated the antibacterial activity of these Cousinia ethanol extracts, using disk diffusion method. Among the ethanol extracts, the total extract of C. sulabadensis elicited signifi cant inhibition of MMP activity in a dose-response fashion (49.2 ± 0.51, p < 0.05). However, this extract exhibited the lowest cytotoxicity effect at all tested concentrations. The concentration necessary to produce a 50% cell death rate (IC50) with C. shulabadensis was 304.5 ± 0.61 μg/mL. The calculated IC50 for cytotoxicity of the other Cousinia species extracts ranged between 18.4 ± 0.59 to 87.9 ± 0.58 μg/mL. The highest antibacterial activity was observed for the total extract of Cousinia phyllocephala. In conclusion, this study supports that Cousinia species display a remarkable inhibition of matrix metalloproteinases activity. The concomitant MMPinhibitory and low cytotoxicity effects observed in C. sulabadensis might coin this extra...

Isolation and Identification of Two Phenolic Compounds from a Moderately Cytotoxic Fraction of Cousinia verbascifolia Bunge

“…demonstrated cytotoxicity and MMP inhibitory effects of C. verbascifolia . [ 18 ] These results together with aforementioned properties of apigenin and caffeic acid may explain the moderate cytotoxicity of C. verbascifolia phenolic fraction.…”

“…In the same study on fibrosarcoma WEHI 164 cancer cells, most of the Cousinia species had considerable inhibitory effect on matrix metalloproteinase (MMP) activity. [ 18 ]…”

Farkli Cousinia Türleri̇nde Psödo Taraksasterol (Ψ-Taraksasterol) Bi̇leşi̇ği̇ni̇n HPLC-Pda İle Mi̇ktar Tayi̇ni̇

“…Some species of this genera were traditionally used for the treatment of various disorders as respiratory problems, ulcers, rheumatism and inflammation (4,5). Based on previous studies cytotoxic, antioxidant, antibacterial, and hypnotic activity of different Cousiniaspecies were reported (4,(6)(7)(8). To date, sesquiterpene lactones (C. Picheriana Bornm.…”

Characterization of Secondary Metabolites in Two Cousinia species

“…The highest activity was observed in C. verbascifolia Bunge. (IC50 = 18.4 ± 0.59 μg / mL) [15]. According to Iranshahy et al sesquiterpene compounds namely desoxyjanerin and raserolit obtained from the dichloromethane extract of C. aitchisonii were subjected cytotoxic screening on five different cell lines.…”

“…In a study by Shahverdi et al the antibacterial effects of ethanol extracts of seven different Cousinia species was investigated by disk diffusion method in different Gram (+) and Gram (-) strains and the highest effect was determined in C. phyllocephala Bornm. & Gauba extract against Staphylococcus aereus and Bacillus subtilis (MIC = 4 mg / disc) [15]. Cousinia iconica Hub.-Mor.…”