Antibiotic, cytotoxic and enzyme inhibitory activity of crude extracts from Brazilian marine invertebrates

Seleghim, Mirna Helena Regali; Lira, Simone Possedente de; Kossuga, M. H.; Batista, Tatiana; Berlinck, Roberto G. S.; Hajdu, Eduardo; Muricy, Guilherme; Rocha, Rosana M.; Nascimento, Gislene Garcia Franco do; Silva, Márcio; Pimenta, Eli F.; Thiemann, Otávio Henrique; Oliva, Glaucius; Cavalcanti, Bruno C.; Pessoa, Cláudia; Moraes, Manoel Odoríco de; Galetti, Fábio C. S.; Silva, Célio Lopes; Souza, Ana Olı́via de; Peixinho, Solange

doi:10.1590/s0102-695x2007000300002

Cited by 41 publications

(40 citation statements)

References 136 publications

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“…Although Brazil has the world's second most extensive coastline after Australia, the development of the chemistry and pharmacology of Brazilian marine organisms has been hampered for many years because the main focus of Brazilian natural product chemists has been directed to the study of medicinal plants and microorganisms. Only recently, a few Brazilian research groups have focused on the chemistry of marine organisms (Torres et al, 2002;Berlinck et al, 2004;Seleghim et al, 2007). Our results add new information to the growing database of known biological activities of Brazilian sponges.…”

Section: Discussionmentioning

confidence: 59%

“…The Arenosclerins A-C and haliclonacyclamine E isolated from the Brazilian sponge Arenosclera brasiliensis inhibited growth of twelve antibiotic-resistant bacteria isolated from a hospital (Torres et al, 2002). Crude extracts of less than 3% of 215 Brazilian sponge species tested showed activity against antibiotic-resistant bacteria (Seleghim et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Marine sponges are among the richest sources of pharmacologically-active chemicals from marine organisms; they are known to produce many different compounds with antitumoral, antiviral, antifungal, and antibacterial activities (e.g., Kobayashi, 2000;Berlinck et al 2004;Blunt et al 2007;Seleghim et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…So far, pure compounds isolated from one specie (Torres et al, 2002) and crude extracts of 215 species of Brazilian sponges have been investigated through bioassays against antibiotic-resistant bacteria (Seleghim et al, 2007). Although a growing number of studies investigated the cytotoxic, neurotoxic and antimicrobial activities of sponges from the Brazilian coast (Muricy et al, 1993;Rangel et al, 2001;Monks et al, 2002;Berlinck et al, 2004;Seleghim et al, 2007), most Brazilian sponges remain unstudied.…”

Section: Introductionmentioning

confidence: 99%

“…Although a growing number of studies investigated the cytotoxic, neurotoxic and antimicrobial activities of sponges from the Brazilian coast (Muricy et al, 1993;Rangel et al, 2001;Monks et al, 2002;Berlinck et al, 2004;Seleghim et al, 2007), most Brazilian sponges remain unstudied. The city of Rio de Janeiro harbours a sponge fauna distinct from that of other areas in Southeastern Brazil such as Arraial do Cabo and São Sebastião, were other screenings have been conducted (Monteiro & Muricy, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges

Marinho¹,

Muricy²,

Silva³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Bactérias resistentes a antibióticos inibidas por extratos e frações de esponjas marinhas do Brasil". O número crescente de bactérias resistentes aos antibióticos tem se tornado um sério problema médico nos últimos anos. As esponjas marinhas são uma fonte rica em compostos bioativos e muitas espécies podem ser úteis para o desenvolvimento de novos antimicrobianos. Esse estudo descreve uma triagem in vitro de esponjas para a pesquisa de novas substâncias contra bactérias resistentes. Os extratos de esponjas foram testados sobre 44 estirpes bacterianas, incluindo quatorze resistentes a antibióticos. Dez entre doze espécies de esponjas apresentaram atividade em um ou mais bioensaios. Os extratos aquosos de Cinachyrella sp. e Petromica citrina apresentaram um amplo espectro de ação sobre estirpes bacterianas resistentes, tais como, Staphylococcus aureus, Staphylococcus coagulase-negativos e Enterococcus faecalis. O extrato aquoso de P. citrina foi fracionado e a fração aquosa apresentou atividade inibitória sobre estirpes de Staphylococcus. Esta fração, na concentração do CMI (16 μg/mL), demonstrou efeito bactericida sobre células de S. aureus na fase exponencial de crescimento. O mecanismo de ação da fração ainda não foi elucidado, mas nós observamos que esta afeta a síntese protéica de Staphylococcus. Nossos resultados demonstraram pela primeira vez que Petromica citrina é uma fonte potencial de novas drogas para o tratamento de infecções causadas por bactérias resistentes. Unitermos: atividade antibacteriana, bactéria resistente a antibióticos, esponjas marinhas, Petromica citrina. ABSTRACT:The growing number of bacterial strains resistant to conventional antibiotics has become a serious medical problem in recent years. Marine sponges are a rich source of bioactive compounds, and many species can be useful for the development of new antimicrobial drugs. This study reports the in vitro screening of marine sponges in the search for novel substances against antibiotic-resistant bacteria. Sponge extracts were tested against 44 bacterial strains, including fourteen antibiotic-resistant strains. Ten out of the twelve sponge species studied showed activity in one or more of the bioassays. Aqueous extracts of Cinachyrella sp. and Petromica citrina showed a large action spectrum over resistant-bacteria such as Staphylococcus aureus, coagulase-negative staphylococci and Enterococcus faecalis. Aqueous extract of P. citrina was fractioned and aqueous fraction showed a greatest inhibitory activity on Staphylococcus strains. In addition, this fraction demonstrated a bactericidal effect on exponentially growing S. aureus cells at the MIC (16 µg/ mL). The mechanism of action of bioactive fraction is still unclear, but we showed that it affect protein biosynthesis of Staphylococcus. Our results demonstrated for the first time that P. citrina is a potential source of new drugs for the treatment of infections by antibiotic-resistant bacteria.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges

Marinho¹,

Muricy²,

Silva³

et al. 2010

Rev. bras. farmacogn.

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Ascidians

Arumugam¹,

Venkatesan²,

Ramachandran³

et al. 2020

Encyclopedia of Marine Biotechnology

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Anti‐inflammatory and antiproliferative activities of crude extract and its fractions of the defensive secretion from the Mediterranean sponge, Spongia officinalis

Dellai

Laroche-Clary

Mhadhebi

et al. 2010

Drug Development Research

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The fact that conventional and newly emerging treatment procedures like chemotherapy, catalytic therapy, photodynamic therapy, and radiotherapy have not succeeded in reversing the outcome of many cancers alternative treatment options has been explored. This study documents the identification of component(s) from the Mediterranean sponge, Spongia officinalis that have anti-inflammatory and antiproliferative activities. In the present study we investigated the efficacy of a crude extract and its semipurified fractions (F1-F3) of the defensive secretion from Spongia officinalis for in vivo anti-inflammatory activity using the carrageenan-induced paw edema assay in rats and their in vitro antiproliferative effects against three human cancer cell lines (A549, lung cell carcinoma; HCT15, colon cell carcinoma; and MCF7, breast adenocarcinoma). Among the series, the crude extract exhibited interesting antiinflammatory activity associated with significant growth and concentration-related colony inhibitory effects against the three cell lines. The fractions F2 and F3 showed, respectively, interesting antiinflammatory and antiproliferative activities in a dose-dependent manner. The purification and the determination of chemical structures of compounds of these active fractions are under investigation. Drug Dev Res 71: 412-418, 2010.

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Antibiotic, cytotoxic and enzyme inhibitory activity of crude extracts from Brazilian marine invertebrates

Cited by 41 publications

References 136 publications

Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges

Antibiotic-resistant bacteria inhibited by extracts and fractions from Brazilian marine sponges

Ascidians

Anti‐inflammatory and antiproliferative activities of crude extract and its fractions of the defensive secretion from the Mediterranean sponge, Spongia officinalis

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