Seasonal variation in the content of tannins in barks of barbatimão species

Santos, Suzana da Costa; Costa, Wesley F; Batista, Fernando Augusto Franco; Santos, Lúcio R.; Ferri, Pedro Henrique; Ferreira, Heleno Dias; Seraphin, José Carlos

doi:10.1590/s0102-695x2006000400019

Cited by 34 publications

(21 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast to previous studies (Scogings et al, 2004;Santos et al, 2006;Fang et al, 2011) the levels of all phenolics did not present any correlation with climatic changes, such as rainfall and temperature. Clusters I and IV have no significant differences in all phenol levels despite occurring in distinct seasons (Table 1).…”

Section: Resultscontrasting

confidence: 99%

“…Tannin and terpenoid amounts alter during the development of the plant and also as a response to environmental changes (Salminen et al, 2001;Salminen et al, 2004;Scogings et al, 2004;Santos et al, 2006;Solar et al, 2006;Fang et al, 2011). Previous studies showed the seasonal influence on the terpenoids composition of the E. uniflora leaves oil (Costa et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of foliar nutrients on phenol levels in leaves of Eugenia uniflora

Santos¹,

Fortes²,

Ferri³

et al. 2011

Rev. bras. farmacogn.

Self Cite

View full text Add to dashboard Cite

Eugenia unifl ora L., Myrtaceae, leaves contain high amounts of phenolic compounds which are responsible for several pharmacological activities. In order to evaluate the phenolics seasonal variation leaves were analysed on a monthly basis during the period of two years for the contents of hydrolysable tannins, total phenols, fl avonoids, and nutrients (N, P, K, S, Ca, Mg, Mn, Zn, Cu, and Fe). Results were correlated with climate conditions (rainfall, humidity, and mean temperature) by Principal Component and Cluster Analysis which allowed four groups to be distinguished with respect to the age of the leaves and the content of some metals. Young leaves were characterised by high levels of Zn and nitrogen whereas old leaves contained high levels of Fe and calcium, and both groups had moderate amounts of phenolics. Adult leaves were divided in two groups and results revealed that while one group had the highest levels of all phenols and lowest amounts of Mn and Cu, the other showed opposite quantities. The Canonical Correlation Analysis confi rmed a highly signifi cant negative correlation between phenol contents and Mn and Cu. These facts suggested that fl avonoids and tannins production depends of the amounts of foliar nutrients, Cu and Mn in particular, which are cofactors of enzymes involved in phenol degradation and lignin biosynthesis. This knowledge can improve this specie cultivation in order to enhance the phenolic compounds concentration.

show abstract

Section: Resultscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of foliar nutrients on phenol levels in leaves of Eugenia uniflora

Santos¹,

Fortes²,

Ferri³

et al. 2011

Rev. bras. farmacogn.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Resultados semelhantes foram encontrados por Silva et al (7) , que confirmaram a presença de saponinas, catequinas, taninos, fenóis e antraquinonas, mas constataram ausência de alcalóides e esteróides/triterpenóides. Silva et al (13) , em estudos fitoquímicos por espectrometria de massa e espectroscopia de ressonância magnética nuclear, revelaram que as catequinas são um componente importante na classe dos taninos condensáveis. Os ensaios da atividade antibacteriana in vitro dos extratos (ECH, EA, EE) da casca de Abarema cochliacarpos mostraram inibição contra a bactéria Gram-positiva Staphylococcus intermedius em todas as concentrações testadas (100, 50, 25, 12,5 e 6,25 mg/mL), exceto para o extrato em ciclohexano, que não demonstrou inibição nas concentrações de 12,5 e 6,25 mg/mL.…”

Section: Resultado E Discussãounclassified

ATIVIDADE ANTIBACTERIANA IN VITRO DO EXTRATO DE Abarema cochliacarpos (GOMES) BARNEBY & J.W. GRIMES CONTRA BACTÉRIAS ISOLADAS DE FERIDAS CUTÂNEAS DE CÃES

et al. 2016

View full text Add to dashboard Cite

ResumoAbarema cochliacarpos é uma espécie nativa do Brasil, pertence à família Leguminosae -Mimosoidae, muito utilizada na medicina popular. Objetivou-se avaliar a atividade antibacteriana in vitro dos extratos ciclohexânico, acetônico e etanólico da casca de Abarema cochliacarpos (Gomes) Barneby & J.W. Grimes contra bactérias isoladas de feridas cutâneas de cães. A atividade antibacteriana dos extratos foi determinada pelo método de difusão em meio sólido enquanto que a Concentração Mínima Inibitória foi determinada em microplacas. Alíquotas dos poços sem crescimento bacteriano após 24h de incubação a 35-37 o C foram adicionadas em placas com meio de cultura para determinação da Concentração Mínima Bactericida. A análise fitoquímica da planta revelou resultados positivos para alcalóides, flavonóides, saponinas, taninos (condensáveis), terpenos e esteróides. As bactérias isoladas foram: Staphylococcus intermedius, Bacillus sp., Pasteurella sp. e Escherichia coli. Os ensaios in vitro dos extratos da casca de Abarema cochliacarpos mostraram inibição contra as bactérias Gram-positivas Staphylococcus intermedius e Bacillus sp. nas concentrações testadas (100, 50, 25, 12,5 e 6,25 mg/mL), exceto para o extrato em ciclohexano que não demonstrou inibição nas concentrações de 12,5 e 6,25 mg/mL contra o Staphylococcus intermedius. Não houve halos de inibição frente aos isolados Gram-negativos Pasteurella sp. e Escherichia coli. Concluiu-se que os extratos ciclohexânico, acetônico e etanólico da casca de Abarema cochliacarpos mostraram atividade antibacteriana, para a maioria das concentrações testadas, frente às cepas Gram-positivas Staphylococcus intermedius e Bacillus sp. isoladas de feridas cutâneas de cão. Palavras-chave: ação antibacteriana; barbatimão; fitoquímica; Mimosoidae; taninos. AbstractAbarema cochliacarpos is a native species of Brazil, it belongs to the family Leguminosae -Mimosoidae, and it has been long used in folk medicine. This study aimed to evaluate the in vitro antibacterial activity of cyclohexane, acetone, and ethanol extracts of the bark of Abarema cochliacarpos (Gomes) Barneby & JW Grimes against bacteria isolated from skin wounds in dogs. The antibacterial activity of the extracts was determined by the diffusion method on solid medium while the Minimal Inhibitory Concentration was determined in microplates. Aliquots of the wells without bacterial growth after 24 hours of incubation at 35-37 o C were added to plates with culture medium to determine Minimum Bactericidal Concentration. The phytochemical analysis of the plant showed positive results for

show abstract

“…The latter has very similar flavonoid content but twice more polyphenol concentration compared to C. sylvestris (0.640 g% and 2.22 g% for flavonoids and polyphenols, respectively); these parameters were measured by the methodology described elsewhere (39). This variation in plant compounds can change medicinal properties (40). Parameters such as ash production, humidity, flavonoids and phenols are important for phytochemical characterization and maintenance of a physicochemical standard of plant extracts.…”

Section: Resultsmentioning

confidence: 99%

Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract

Camargo¹,

Nazato²,

Silva³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Abstract:The hydroalcoholic extract of Casearia gossypiosperma Briquet (Flacourtiaceae) was standardized for the first time through quality control procedures including pharmacognostic methods, fingerprint chromatograms, defined amounts of marker substances and physicochemical characteristics. The pharmacological activity of C. gossypiosperma (Cg) hydroalcoholic extract was assayed by a traditional in vitro test, which involved irreversible neuromuscular blockade induced by Bothrops jararacussu (Bjssu) venom (60 μg/mL) in mouse phrenic nerve-diaphragm preparations. Bjssu venom blocked muscle activity for 26 (± 2.0) minutes (n = 6). Cg extract (0.1 mg/mL) induced changes on the baseline muscle activity without impairing the muscle function and inhibited 87.6% (± 1.8) (n = 6) of the Bjssu venom-induced blockade. Both flavonoids (0.624 g%) and polyphenols (4.63 g%) from the extract were spectrophotometrically quantified. Therefore, the present study confirms the antibothropic activity of Cg extract, supporting the ethnomedical use of Casearia sp. in the treatment of snakebite victims.Key words: antibothropic extract, Bothrops jararacussu, Casearia gossypiosperma Briquet, snake venoms. pharmacologically assessed for the first time. Original PaPer The Journal of Venomous Animals and Toxins including Tropical DiseasesIn vivo myotoxic effects and in vitro irreversible neuromuscular blockade effects of crude venom from the snake Bothrops jararacussu are well known pharmacological methods used to study drugs showing antivenom properties (29,30). Thus, the aim of the present study was to verify the capability of Casearia gossypiosperma hydroalcoholic extract to neutralize the neuromuscular blockade induced by Bothrops jararacussu venom. MATERIALS AND METHODS Plant Material and ExtractionLeaves from an adult C. gossypiosperma Briquet (Cg) tree were collected from the herbarium at the Higher School of Agriculture Luiz de Queiroz (ESALQ) of the University of São Paulo, Piracicaba, São Paulo, Brazil, in January 2006; the species was previously identified in 1988 (protocol IAC 38115). Cg leaves were dried at 35°C to 40°C for 24 hours. The dried leaves were then powdered, ground in a mill, macerated (200 g, during five days) in 2 L of 70% ethanol and the suspension was percolated (under protection against light) at 20 drops/minute, resulting in a 10% (m/v) hydroalcoholic extract. Quality Control Assays of the Medicinal Plants Ash and humidity testsTo observe elementary physical and chemical characteristics, Cg powder was subjected to ash and humidity tests (31). Briefly, 100 g of the specimen powder were placed in six calibrated melting pots, which were warmed until total carbonization of the powders. The melting pots were kept at 650°C and the ashes were then weighed. Results are expressed as grams of ashes/100 g of sample. The humidity test was performed by placing 1 g of specimen powder in six calibrated porcelain capsules, which were heated at 105ºC during four hours and then weighed. Thin Layer Chromatography (TLC)Aliquots...

show abstract

Seasonal variation in the content of tannins in barks of barbatimão species

Cited by 34 publications

References 18 publications

Influence of foliar nutrients on phenol levels in leaves of Eugenia uniflora

Influence of foliar nutrients on phenol levels in leaves of Eugenia uniflora

ATIVIDADE ANTIBACTERIANA IN VITRO DO EXTRATO DE Abarema cochliacarpos (GOMES) BARNEBY & J.W. GRIMES CONTRA BACTÉRIAS ISOLADAS DE FERIDAS CUTÂNEAS DE CÃES

Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract

Contact Info

Product

Resources

About