Atividade da mieloperoxidase está aumentada na síndrome hepatopulmonar em ratos

Nacif, Lucas Souto; Andraus, Wellington; Kubrusly, Márcia Saldanha; Molan, Nilza A.; Chaib, Eleazar; D’Albuquerque, Luiz Augusto Carneiro

doi:10.1590/s0102-67202013000400008

Cited by 4 publications

(2 citation statements)

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“…The MPO enzyme of neutrophils and other cells of myeloid origin is responsible for catalyzing the production of hypochlorous acid that kill the parasites. Studies have assigned the dosage of the MPO enzyme as an indirect indicator of neutrophil presence/activation in tissues [ 47 , 48 ]. In our study, the MPO activity increased only in the duodenum at 12 h of infection.…”

Section: Discussionmentioning

confidence: 99%

Immunocompetent host develops mild intestinal inflammation in acute infection with Toxoplasma gondii

et al. 2018

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Toxoplasma gondii (T. gondii) is the causative agent of toxoplasmosis, common zoonosis among vertebrates and high incidence worldwide. During the infection, the parasite needs to transpose the intestinal barrier to spread throughout the body, which may be a trigger for an inflammatory reaction. This work evaluated the inflammatory alterations of early T. gondii infection in peripheral blood cells, in the mesenteric microcirculation, and small intestinal tissue by measurement of MPO (myeloperoxidase) activity and NO (nitric oxide) level in rats. Animals were randomly assigned into control group (CG) that received saline orally and groups infected with 5,000 oocysts for 6 (G6), 12 (G12), 24 (G24), 48 (G48) and 72 hours (G72). Blood samples were collected for total and differential leukocyte count. Intravital microscopy was performed in the mesentery to evaluate rolling and adhesion of leukocytes. After euthanasia, 0.5cm of the duodenum, jejunum and ileum were collected for the determination of MPO activity, NO level and PCR to identify the parasite DNA and also the mesentery were collected to perform immunohistochemistry on frozen sections to quantify adhesion molecules ICAM-1, PECAM-1 and P-Selectin. The parasite DNA was identified in all infected groups and there was an increase in leukocytes in the peripheral blood and in expression of ICAM-1 and PECAM-1 in G6 and G12, however, the expression of P-selectin was reduced in G12. Leukocytes are in rolling process during the first 12 hours and they are adhered at 24 hours post-infection. The activity of MPO increased in the duodenum at 12 hours, and NO increased in the jejunum in G72 and ileum in G24, G48 and G72. Our study demonstrated that T. gondii initiates the infection precociously (at 6 hours) leading to a systemic activation of innate immune response resulting in mild inflammation in a less susceptible experimental model.

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Section: Discussionmentioning

confidence: 99%

Immunocompetent host develops mild intestinal inflammation in acute infection with Toxoplasma gondii

et al. 2018

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“…It was also seen in both genders in middle-aged patients 17 . HPS pathogenesis is not well defined, but it is speculated that a combination of factors, such as an imbalance in the response of vascular endothelin receptors, pulmonary microvascular remodeling and genetic predisposition, leads to intrapulmonary vascular dilatation and bacterial translocation 1 , 10 , 12 , 14 , 17 , 19 .…”

Section: Introductionmentioning

confidence: 99%

The hepatopulmonary syndrome

Nacif¹,

Andraus²,

Pinheiro³

et al. 2014

ABCD, arq. bras. cir. dig.

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IntroductionThe hepatopulmonary syndrome has been acknowledged as an important vascular complication in lungs developing systemic hypoxemia in patients with cirrhosis and portal hypertension. Is formed by arterial oxygenation abnormalities induced from intrapulmonary vascular dilatations with liver disease. It is present in 4-32% of patients with cirrhosis. It increases mortality in the setting of cirrhosis and may influence the frequency and severity. Initially the hypoxemia responds to low-flow supplemental oxygen, but over time, the need for oxygen supplementation is necessary. The liver transplantation is the only effective therapeutic option for its resolution.AimTo update clinical manifestation, diagnosis and treatment of this entity.MethodA literature review was performed on management of hepatopulmonary syndrome. The electronic search was held of the Medline-PubMed, in English crossing the headings "hepatopulmonary syndrome", "liver transplantation" and "surgery". The search was completed in September 2013.ResultsHepatopulmonary syndrome is classically defined by a widened alveolar-arterial oxygen gradient (AaPO2) on room air (>15 mmHg, or >20 mmHg in patients >64 years of age) with or without hypoxemia resulting from intrapulmonary vasodilatation in the presence of hepatic dysfunction or portal hypertension. Clinical manifestation, diagnosis, classification, treatments and outcomes are varied.ConclusionThe severity of hepatopulmonary syndrome is an important survival predictor and determine the improvement, the time and risks for liver transplantation. The liver transplantation still remains the only effective therapeutic.

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