O modelo experimental de carcinogênese gástrica induzido por n-methyl-n-nitrosourea em ratos F344 e camundongos C3H é válido para os ratos Wistar?

Tarso, Lissandro; Meyer, Fabíola Schons; Cioato, Marta Justina Giotti; Meurer, Luíse; Schirmer, Carlos Cauduro

doi:10.1590/s0102-67202011000100012

Cited by 2 publications

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“…Duodenogastric reflux is, in a sense, normal in post-prandial event, and therefore its pathogenicity depends on volume and duration of exposure to gastric contents. Experimental studies in rats show that duodenogastric reflux produces glandular stomach adenocarcinoma, squamous cell carcinoma in the pre-stomach, and promotes the development of Barrett's esophagus, esophageal adenocarcinoma and squamous cell carcinoma of the esophagus 10,12,18,25,34,35,37 . Carcinoma of the stomach is a rare spontaneous cancer in animals.…”

Section: Introductionmentioning

confidence: 99%

Modelo de carcinogênese gástrica utilizando piloroplastia de Finney: estudo experimental em ratos

Kaminski¹,

Kruel²

2011

ABCD, arq. bras. cir. dig.

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-Background: The duodenogastric reflux has been implicated as a potential carcinogen for the stomach and esophagus and is one of the factors that may explain the development of gastric stump cancer. Experimental models of carcinogenesis in the stomach stump or in the duodenogastric anastomosis are well defined. Aim -To develop an experimental model of gastric carcinogenesis through the Finney pyloroplasty, evaluate the influence of ingestion of sodium nitrite in this model, analyze the concentrations of bile acids and the pH of the stomach. Methods -A hundred and ten Wistar rats were operated and divided into four groups: Group I (15 rats) underwent laparotomy (Sham group); Group II (15 rats) underwent laparotomy (Sham) and ingestion of sodium nitrite in drinking water; Group III (40 rats) submitted to the Finney pyloroplasty and Group IV (40 rats) submitted to the Finney pyloroplasty and ingestion of sodium nitrite in drinking water. After 50 weeks of surgery, the rats were sacrificed and samples collected for analysis of gastric pH, dosing of bile acids and histological analysis. Results -The immediate postoperative mortality was 9%, and during the experiment, 10 rats died. The control group (I) did not show gastric lesions; the control group with sodium nitrite (II) developed papillomas in the pre-stomach in 16.6%; the operated groups with pyloroplasty had adenomas in 10.3% in Group III and 14.2 % in Group IV, and adenocarcinoma in 55.1% in group III and 14.2% in Group IV. The implementation of glands into the submucosa and muscle in the area of anastomosis (mucosa deployment) was not sufficient criterion for deciding on the malignancy of the lesions, requiring the simultaneous presence of atypical cells. The concentration of bile acids in gastric juice was higher in Groups III and IV. The measurement of gastric pH was not different in both groups. Conclusion -1) The Finney pyloroplasty is suitable experimental model of gastric carcinogenesis; 2) it induced duodenogastric reflux; 3) the duodenogastric reflux served as a carcinogen for the stomach; 4) there was no relationship between pH and the development of gastric carcinoma; 5 ) sodium nitrite did not act as a carcinogen for the stomach of rats. ABCDDV/816RESUMO -Racional -O refluxo duodenogástrico tem sido implicado como potencial carcinógeno para o estômago e esôfago e é um dos fatores que podem explicar o desenvolvimento de câncer no coto gástrico. Modelos experimentais de carcinogênese no estômago ressecado ou nas gastrojejunoanastomoses estão bem definidos. Objetivos -Desenvolver um modelo experimental de carcinogênese gástrica através de piloroplastia à Finney, avaliar a influência da ingestão de nitrito de sódio nesse modelo, analisar as concentrações de ácidos biliares e o valor do pH gástrico. Métodos -Foram operados 110 ratos Wistar divididos em quatro grupos: Grupo I (15 ratos) submetidos à laparotomia (grupo Sham); Grupo II (15 ratos) submetidos à laparotomia (Sham) e à ingestão de nitrito de sódio na água de beber; Grupo III (40 ratos...

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Section: Introductionmentioning

confidence: 99%

Modelo de carcinogênese gástrica utilizando piloroplastia de Finney: estudo experimental em ratos

Kaminski¹,

Kruel²

2011

ABCD, arq. bras. cir. dig.

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N-Methyl-N-nitrosourea as a mammary carcinogenic agent

et al. 2015

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The administration of chemical carcinogens is one of the most commonly used methods to induce tumors in several organs in laboratory animals in order to study oncologic diseases of humans. The carcinogen agent N-methyl-N-nitrosourea (MNU) is the oldest member of the nitroso compounds that has the ability to alkylate DNA. MNU is classified as a complete, potent, and direct alkylating compound. Depending on the animals' species and strain, dose, route, and age at the administration, MNU may induce tumors' development in several organs. The aim of this manuscript was to review MNU as a carcinogenic agent, taking into account that this carcinogen agent has been frequently used in experimental protocols to study the carcinogenesis in several tissues, namely breast, ovary, uterus, prostate, liver, spleen, kidney, stomach, small intestine, colon, hematopoietic system, lung, skin, retina, and urinary bladder. In this paper, we also reviewed the experimental conditions to the chemical induction of tumors in different organs with this carcinogen agent, with a special emphasis in the mammary carcinogenesis.

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O modelo experimental de carcinogênese gástrica induzido por n-methyl-n-nitrosourea em ratos F344 e camundongos C3H é válido para os ratos Wistar?

Cited by 2 publications

References 17 publications

Modelo de carcinogênese gástrica utilizando piloroplastia de Finney: estudo experimental em ratos

Modelo de carcinogênese gástrica utilizando piloroplastia de Finney: estudo experimental em ratos

N-Methyl-N-nitrosourea as a mammary carcinogenic agent

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