Toxicidade e acúmulo de flúor em hortaliças nas adjacências de uma fábrica de alumínio

Sant’Anna-Santos, Bruno Francisco; Azevedo, Aristéa Alves

doi:10.1590/s0102-33062010000400010

Cited by 13 publications

(1 citation statement)

References 32 publications

(51 reference statements)

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“…Callus tissue could be recognized in our work by its disorganized aspect, with a certain disaggregation and green staining with toluidine blue, generally indicating phenolic compounds in the cells [49]. Feder and O'Brien (1968) reported that toluidine blue will stain polyphenol containing cells a green color [50]; two studies with Spondias dulcis likewise reported that accumulations of phenolic compounds in the cells were stained green by toluidine blue [51,52], and the same staining was observed in a study with Brassica oleracea [53].…”

Section: Discussionsupporting

confidence: 80%

Morphological and anatomical insights into de novo shoot organogenesis of in vitro ‘Delite’ rabbiteye blueberries

Schuchovski

Sant’Anna-Santos

Marra

et al. 2020

Heliyon

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Blueberries are valued for their taste and their high nutritional benefits, including their antioxidant and antiinflammatory properties. In vitro culturing is an alternative method for clonal propagation, and has been used in many biotechnological studies. Most blueberry research is concentrated on highbush and lowbush taxa (Vaccinium corymbosum and Vaccinium angustifolium respectively), with only limited investigations of rabbiteye cultivars (Vaccinium virgatum) that are more suitable for subtropical climates and regions with warmer winters as a result of climate change. There is therefore a need to determine in vitro protocols for that species and group of cultivars. We examined here adventitious shoot regeneration in the 'Delite' rabbiteye blueberry cultivar. Leaf explants were cultured in vitro in Woody Plant Medium (WPM), and the effects of different thidiazuron (TDZ) concentrations, the orientation of the leaf (adaxial or abaxial surface in contact with the medium), and two portions of the leaf segment (basal or apical) were examined. De novo shoot development was studied using light and scanning electron microscopy. All concentrations of TDZ used showed similar survival and regeneration rates; 0.5 μM TDZ showed high efficiency in regenerating adventitious shoots (100%, with 57 adventitious shoots/ explant), as did the adaxial surface in contact with the medium using either the apical or the basal portion of the leaf (97% shoot regeneration, 47.5 adventitious shoots/explant). Anatomical analyses showed direct and indirect organogenesis. The shoots developed leaf primordia with stomata, trichomes, and well-developed vascular tissues, with further elongation and rooting of the plants. We therefore describe here a high-efficiency regeneration method through de novo shoot organogenesis using TDZ in foliar explants of rabbiteye blueberry, with direct and indirect organogenesis.

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Section: Discussionsupporting

confidence: 80%

Morphological and anatomical insights into de novo shoot organogenesis of in vitro ‘Delite’ rabbiteye blueberries

Schuchovski

Sant’Anna-Santos

Marra

et al. 2020

Heliyon

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Responses of tropical legumes from the Brazilian Atlantic Rainforest to simulated acid rain

Andrade

Silva

2016

Protoplasma

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We investigated the morphological and anatomical effects of simulated acid rain on leaves of two species native to the Brazilian Atlantic Rainforest: Paubrasilia echinata and Libidibia ferrea var. leiostachya. Saplings were subjected to acid rain in a simulation chamber during 10 days for 15 min daily, using HSO solution pH 3.0 and, in the control, deionized water. At the end of the experiment, fragments from young and expanding leaves were anatomically analyzed. Although L. ferrea var. leiostachya leaves are more hydrophobic, rain droplets remained in contact with them for a longer time, as in the hydrophilic P. echinata leaves, droplets coalesce and rapidly run off. Visual symptomatology consisted in interveinal and marginal necrotic dots. Microscopic damage found included epicuticular wax flaking, turgor loss and epidermal cell shape alteration, hypertrophy of parenchymatous cells, and epidermal and mesophyll cell collapse. Formation of a wound tissue was observed in P. echinata, and it isolated the necrosis to the adaxial leaf surface. Acid rain increased thickness of all leaf tissues except spongy parenchyma in young leaves of L. ferrea var. leiostachya, and such thickness was maintained throughout leaf expansion. To our knowledge, this is the first report of acidity causing increase in leaf tissue thickness. This could represent the beginning of cell hypertrophy, which was seen in visually affected leaf regions. Paubrasilia echinata was more sensitive, showing earlier symptoms, but the anatomical damage in L. ferrea var. leiostachya was more severe, probably due to the higher time of contact with acid solution in this species.

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Use of microalga Asterarcys quadricellularis in common bean

Marques,

Mógor,

Amatussi

et al. 2023

J Appl Phycol

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Toxicidade e acúmulo de flúor em hortaliças nas adjacências de uma fábrica de alumínio

Cited by 13 publications

References 32 publications

Morphological and anatomical insights into de novo shoot organogenesis of in vitro ‘Delite’ rabbiteye blueberries

Morphological and anatomical insights into de novo shoot organogenesis of in vitro ‘Delite’ rabbiteye blueberries

Responses of tropical legumes from the Brazilian Atlantic Rainforest to simulated acid rain

Use of microalga Asterarcys quadricellularis in common bean

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