Técnicas para a obtenção de preparações citológicas com alta freqüência de metáfases mitóticas em plantas: Passiflora (Passifloraceae) e Crotalaria (Leguminosae)

Cuco, Silvia Marina; Mondin, Mateus; Vieira, Maria Lúcia Carneiro; Aguiar-Perecin, Margarida Lopes Rodrigues de

doi:10.1590/s0102-33062003000300004

Cited by 19 publications

(16 citation statements)

References 18 publications

(18 reference statements)

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“…In later works, SOARES-SCOTT related the presence of at least three SC, but did not specify their position (cited by SOUZA et al 2003). CUCO et al (2003) observed the presence of SC in chromosomes 8 and 9. According to MAYEDA (1997) differences on chromosome characterization may occur as a result of either the methods used or the quality of the sample preparation.…”

Section: Discussionmentioning

confidence: 99%

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Morphological aspects ofPassiflora edulisf.flavicarpachromosomes using acridine orange banding and rDNA-FISH tools

et al. 2008

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Section: Discussionmentioning

confidence: 99%

“…The latter author later related the presence of at least three SC, as cited by SOUZA et al (2003). In contrast, CUCO et al (2003) observed the presence of SC in chromosomes 8 and 9.…”

Section: Introductionmentioning

confidence: 90%

Morphological aspects ofPassiflora edulisf.flavicarpachromosomes using acridine orange banding and rDNA-FISH tools

et al. 2008

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“…Satellites were classified according to Battaglia's terminology (1955). The length of the satellites was not added to the length of the corresponding chromosome arm but was added to the total length of the chromosome (Cuco et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

Karyomorphology of Caesalpinia Species (Caesalpinioideae: Fabaceae) from Caatinga and Mata Atlantica Biomes of Brazil

Rodrigues¹,

Souza²,

Corrêa³

2012

JPS

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Out of 140 Caesalpinia s.l. species, only 20 species have the chromosome numbers presently known, and nine species have the karyomorphological studies available. We determine the karyotype and the chromosome morphometry in five Caesalpinia s.l. spp., and we describe the heterochromatin pattern in four of them. The diploid chromosome number of 24 was reported for the first time in Caesalpinia calycina, Caesalpinia microphylla and Caesalpinia pluviosa var. peltophoroides, and confirmed in Caesalpinia ferrea var. leiostachya and Caesalpinia pulcherrima. Different karyotype formulae were obtained for each of these five species. The chromosome asymmetry index varied from 34.94 % in C. pluviosa to 39.45 % in C. ferrea. The average chromosome length among the five species differed significantly (P<0.010). The heterochromatin blocks were evidenced by C-banding in both terminal and proximal regions of the Caesalpinia spp. chromosomes. This study represents a contribution toward an increased knowledge on cytogenetics and evolution of this genus.

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“…For chromosome morphology analysis, the roots were stained by the Feulgen method as described by Cuco et al(2003). Root tips used for chromosome banding and FISH preparations were digested in a mixture of 2% (w/v) cellulase (final concentration: 9.2 units mL -1 ) and 0.3% (w/v) pectinase (final concentration: 14.7 units mL -1 ) in citrate buffer (pH 4.6) at 37°C for 1 h and then squashed in 60% (v/v) acetic acid under a coverslip, which was removed in liquid nitrogen.…”

Section: Plant Materials and Chromosome Preparationmentioning

confidence: 99%

Karyotype characterization of Crotalaria juncea (L.) by chromosome banding and physical mapping of 18S-5.8S-26S and 5S rRNA gene sites

2007

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The chromosomes of Crotalaria juncea, a legume of agronomic interest with a 2n = 16 karyotype composed of metacentric chromosomes, were analyzed using several cytogenetic techniques. C-banding revealed heterochromatic regions around the centromeres in all chromosomes and adjacent to the secondary constriction on the chromosome 1 short arm. Fluorescent staining with the GC-specific chromomycin A3 (CMA) highlighted these heterochromatic regions and a tiny site on the chromosome 1 long arm while the AT-specific stain 4'-6-diamidino-2-phenylindole (DAPI) induced a reversed pattern. Staining with CMA combined with AT-specific distamycin A (DA) counterstaining quenched the pericentromeric regions of all chromosomes, but enhanced fluorescence was observed at the heterochromatic regions around the secondary constriction and on the long arms of chromosomes 1 and 4. Fluorescence in situ hybridization (FISH) revealed 18S-5.8S-26S rRNA gene sites (45S rDNA) on chromosomes 1 and 4, and one 5S rDNA locus on chromosome 1. All the rDNA sites were co-located with the positive-CMA/DA bands, suggesting they were very rich in GC. Silver staining revealed signals at the main 45S rDNA locus on chromosome 1 and, in some cells, chromosome 4 was labeled. Two small nucleoli were detected in a few interphase cells, suggesting that the minor site on chromosome 4 could be active at some stages of the cell cycle.

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Técnicas para a obtenção de preparações citológicas com alta freqüência de metáfases mitóticas em plantas: Passiflora (Passifloraceae) e Crotalaria (Leguminosae)

Cited by 19 publications

References 18 publications

Morphological aspects ofPassiflora edulisf.flavicarpachromosomes using acridine orange banding and rDNA-FISH tools

Morphological aspects ofPassiflora edulisf.flavicarpachromosomes using acridine orange banding and rDNA-FISH tools

Karyomorphology of Caesalpinia Species (Caesalpinioideae: Fabaceae) from Caatinga and Mata Atlantica Biomes of Brazil

Karyotype characterization of Crotalaria juncea (L.) by chromosome banding and physical mapping of 18S-5.8S-26S and 5S rRNA gene sites

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