Lutzomyia longipalpis naturally infected by Leishmania (L.) chagasi in Várzea Grande, Mato Grosso State, Brazil, an area of intense transmission of visceral leishmaniasis

Abstract

The American visceral leishmaniasis (AVL) is caused by parasites belonging to the genus Leishmania (Trypanosomatidae) and is transmitted to humans through the bite of certain species of infected phlebotomine sand flies. In this study, we investigated the natural infection ratio of Lutzomyia longipalpis, the main vector species of AVL in Brazil, in Várzea Grande, Mato Grosso State. Between July 2004 and June 2006, phlebotomine sand flies were captured in peridomestic areas using CDC light-traps. Four hundred and twenty (420) specimens of Lu. longipalpis were captured. 42 pools, containing 10 specimens of Lu. longipalpis each, were used for genomic DNA extraction and PCR (polymerase chain reaction) amplification. Leishmania spp. DNA was detected in three out of the 42 pools tested, resulting in a minimal infection ratio of 0.71%. Restriction fragment length polymorphism (RFLP) analysis indicated that Leishmania (L.) chagasi was the infective agent in the positive pools.

Detection of Leishmania (L.) infantum in stray dogs by molecular techniques with sensitive species-specific primers

“…lesions of cats (Vides et al 2011) or dogs (Savani et al 2005;Queiroz et al 2011) from endemic areas, or nonendemic areas (Braga et al 2014), and research in phlebotomines (Missawa et al 2010;Michalsky et al 2011).…”

“…The parasite is transmitted to vertebrate hosts by infected blood-sucking Phlebotominae sand flies of the Lutzomyia longipalpis (Lu. longipalpis) species, which is the main vector of VL in Brazil (Missawa et al 2010;Michalsky et al 2011). VL is also endemic in dogs from Mediterranean countries, i.e.…”

Ecoepidemiología de la Leishmaniasis cutánea no ulcerada en Honduras

“…evansi y Lu. cruciata, lo que concuerda con estudios realizados en Centroamérica y Suramérica (Gonzalez, 2014;Missawa, 2010;Soares, 2010).…”

Capacidade vetorial de Lutzomyia (Lutzomyia) cruzi (Diptera: Psychodidae) para Leishmania (Leishmania) infantum

“…cruzi, muitos autores têm relatado taxas de infecção por Leishmania inferiores a 10% usando diferentes metodologias (ACARDI et al, 2010;LAINSON et al, 1985;MIRANDA et al, 2002;MISSAWA et al, 2010;PAIVA et al, , 2010SANAVI et al, 2009;SILVA et al, 2008;SANT'ANNA et al, 2008 (Mangabeira, 1942), não tem sido descrito na região SANTOS et al, 1988 (BRASIL, 2007;LAINSON e SHAW, 1987, 2005.…”