Detección y diferenciación de Entamoeba histolytica y Entamoeba dispar mediante reacción en cadena de la polimerasa en individuos de una comunidad del Estado Zulia, Venezuela

Rivero, Zulbey; Bracho, Ana; Calchi, Marinella; Díaz, Ibraín Piloto; Acurero, Ellen; Maldonado, Adrián; Chourio, G.; Arraíz, Nailet; Corzo, Gilbert

doi:10.1590/s0102-311x2009000100016

Cited by 11 publications

(8 citation statements)

References 24 publications

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“…E. histolytica was found in 7% of the cases and was diagnosed by highly specific PCR, which is able to distinguish between E. histolytica and the pathogen forms E. dispar/moshkovskii [ 21 , 22 ]. Compared to studies performed in rural areas of Venezuela [ 23 ] and Mexico [ 24 ], where a prevalence of 11% and 14% was respectively detected, the occurrence rate among the Wiwas seems to be lower. The different diagnostic techniques applied (microscopy and different PCRs) might have affected these findings.…”

Section: Discussioncontrasting

confidence: 84%

High Prevalence of Intestinal Pathogens in Indigenous in Colombia

Kann

Bruennert

Hansen

et al. 2020

JCM

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Background: Intestinal infections remain a major public health burden in developing countries. Due to social, ecological, environmental, and cultural conditions, Indigenous peoples in Colombia are at particularly high risk. Materials: 137 stool samples were analyzed by microscopy and real-time-Polymerase Chain Reaction (RT-PCR), targeting protozoan parasites (Giardia intestinalis, Entamoeba histolytica, Cryptosporidium spp., and Cyclospora cayetanensis), bacteria (Campylobacter jejuni, Salmonella spp., Shigella ssp./enteroinvasive E. coli (EIEC), Yersinia spp., enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), enterotoxin-producing E. coli (ETEC), enteroaggregative E. coli (EAEC), and Tropheryma whipplei), and helminths (Necator americanus, Strongyloides stercoralis, Ascaris lumbricoides, Ancylostoma spp., Trichuris. trichiura, Taenia spp., Hymenolepis nana, Enterobius vermicularis, and Schistosoma spp.). Microscopy found additional cases of helminth infections. Results: At least one pathogen was detected in 93% of the samples. The overall results revealed protozoa in 79%, helminths in 69%, and bacteria in 41%. G. intestinalis (48%), Necator/hookworm (27%), and EAEC (68%) were the most common in each group. Noteworthy, T. whipplei was positive in 7% and T. trichirua in 23% of the samples. A significant association of one infection promoting the other was determined for G. intestinalis and C. jejuni, helminth infections, and EIEC. Conclusions: The results illustrate the high burden of gastrointestinal pathogens among Indigenous peoples compared to other developing countries. Countermeasures are urgently required.

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Section: Discussioncontrasting

confidence: 84%

High Prevalence of Intestinal Pathogens in Indigenous in Colombia

Kann

Bruennert

Hansen

et al. 2020

JCM

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“…Otra porción se congeló a -20 °C, hasta su procesamiento por técnicas de biología molecular, en las que se hicieron la extracción de ADN, su purificación y luego la amplificación mediante reacción en cadena de la polimerasa (PCR) en forma separada para E. histolytica y E. dispar, según procedimiento de Rivero, et al (13).…”

Section: Materiales Y Métodosunclassified

Prevalencia de microsporidios intestinales y otros enteroparasitos en pacientes VIH positivos de Maracaibo, Venezuela

et al. 2013

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“…histolytica is more frequent than E . dispar [ 22 , 23 , 24 ]. In Colombia there is no data (by molecular methods) on the prevalence of these three amoebas in humans and also if there exist the presence of E .…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Entamoeba: First Description of Entamoeba moshkovskii in a Rural Area from Central Colombia

et al. 2015

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Background Entamoeba histolytica, E. dispar and E. moshkovskii are the most frequent species described in human infection where E. histolytica is the only true pathogen. The epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the Entamoeba species complex. Therefore, molecular methods appear as the unique epidemiological tool to accomplish the species discrimination. Herein, we conducted a cross-sectional study to determine the frequency of Entamoeba species infections in a group of asymptomatic individuals from a rural area in central Colombia.Methodology/Principal FindingsA total of 181 fecal samples from asymptomatic children under 16 years old from the hamlet La Vírgen, Cundinamarca (Colombia) that voluntarily accepted to participate in the study were collected. The fecal samples were examined by light microscopy and DNA-extracted, subsequently submitted to molecular discrimination of E. dispar/E. histolytica/E. moshkovskii infection based on a multiplex PCR assay targeting the 18S rRNA fragment. To confirm the species description, twenty samples were randomly submitted to DNA sequencing of the aforementioned fragment. By direct microscopic examination, frequency of the complex E. histolytica/E. dispar/E. moshkovskii was 18.8% (34/181). PCR showed a frequency of 49.1% (89/181), discriminated as 23.2% (42/181) that were positive for E. dispar, 25.4% (46/181) for E. moshkovskii and 0.55% (1/ 181) for E. histolytica. Also, mixed infections were detected between E. dispar and E. moshkovskii at 4.42% (8/181) of the samples. Molecular barcoding confirmed the diagnosis depicted by the multiplex PCR assay.Conclusions/SignificanceThis is the first description of E. moshkovskii in Colombia and the second report in South-America to our knowledge. Our results suggest the need to unravel the true epidemiology of Entamoeba infections around the world, including the real pathogenic role that E. moshkovskii may have.

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Detección y diferenciación de Entamoeba histolytica y Entamoeba dispar mediante reacción en cadena de la polimerasa en individuos de una comunidad del Estado Zulia, Venezuela

Cited by 11 publications

References 24 publications

High Prevalence of Intestinal Pathogens in Indigenous in Colombia

High Prevalence of Intestinal Pathogens in Indigenous in Colombia

Prevalencia de microsporidios intestinales y otros enteroparasitos en pacientes VIH positivos de Maracaibo, Venezuela

Molecular Epidemiology of Entamoeba: First Description of Entamoeba moshkovskii in a Rural Area from Central Colombia

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