C677T polymorphism of the MTHFR gene and variant hemoglobins: a study in newborns from Salvador, Bahia, Brazil

Couto, Fábio David; Adôrno, Elisângela Vitória; Menezes, Joelma Figueiredo; Neto, José Pereira Moura; Rêgo, Marco Antônio Vasconcelos; Reis, Mitermayer Galvão dos; Gonçalves, Marilda Souza

doi:10.1590/s0102-311x2004000200021

Cited by 18 publications

(9 citation statements)

References 22 publications

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“…Cord blood samples were carried out only after signed informed consent form was obtained. We carried out a cross-sectional transversal study comprising 143 newborn babies randomly selected from a group of 843 neonates, as reported (Couto et al, 2004). The babies were born from February to December 2000 in two different maternity hospitals, the Tsylla Balbino, a public maternity hospital and the Santo Amaro, a private maternity hospital located in city of Salvador.…”

Section: Subjectsmentioning

confidence: 99%

Folate, vitamin B12 and total homocysteine levels in neonates from Brazil

Couto

Moreira²,

Santos

et al. 2006

Eur J Clin Nutr

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Objective: To determine folates, vitamin B12 and total homocysteine levels among neonates from mothers of low or high socioeconomic status. Design: We carried out a cross-sectional transversal study comprising 143 neonates from two maternity hospitals in the city of Salvador, Northeast of Brazil. Cord blood samples were obtained at the time of delivery from newborns from low (group 1, n ¼ 77) or high (group 2, n ¼ 66) socioeconomic status. The vitamin B12 and folates were analyzed by electrochemiluminescence immunoassay and by a competitive test using a natural folate-binding protein (FBP), respectively. Total homocyteine levels were measured by fluorescence polarization immunoassay. Maternal environmental risk factors for pregnancy complications were obtained from all mothers. Results: Only 2% of women from group 1 received prenatal care/vitamin supplementation, whereas almost all mothers from group 2 (96%) were properly followed. Anemia and/or infections pre-or during pregnancy was more prevalent among mothers of babies from group 1. Folate levels among newborns from group 1 and 2 were 7.3872.71 and 8.8374.06 ng/ml, respectively. No difference in the vitamin B12 levels was determined between groups. In addition, tHcy serum levels were higher among newborns from group 1 compared to those from group 2 (8.5474.06 vs 6.3571.33 mmol/l, respectively; P ¼ 0.005).Conclusion: These results demonstrate that unprivileged young woman has limited accesses to prenatal care, present high-risk factors that hamper both maternal and newborn health. Maternal and newborn health status could be improved by simply reinforcing the use of folate-enriched diet. The work presented illustrates the challenges that developing countries have to face in order to provide preventive adequate health care to the population at large.

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Section: Subjectsmentioning

confidence: 99%

Folate, vitamin B12 and total homocysteine levels in neonates from Brazil

Couto

Moreira²,

Santos

et al. 2006

Eur J Clin Nutr

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“…The T677 allele frequency and TT677 genotype was higher than those observed in other studies of African-descent populations. The T allele frequency was 0.23 and the C/T and T/T genotypes prevalence were 36.2 and 5.3 percent, respectively [29]. …”

Section: Resultsmentioning

confidence: 99%

Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and high plasma homocysteine in chronic hepatitis C (CHC) infected patients from the Northeast of Brazil

Siqueira

Oliveira

Muniz

et al. 2011

Nutr J

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Background/AimHyperhomocysteinemia due to Methylenetetrahydrofolate Reductase (MTHFR) gene, in particular the C677T (Ala222Val) polymorphism were recently associated to steatosis and fibrosis. We analyzed the frequency of MTHFR gene in a cross-sectional study of patients affected by Chronic Hepatitis C (CHC) from Northeast of Brazil.MethodOne hundred seven-four untreated patients with CHC were genotyped for the C677T MTHFR. Genomic DNA was extracted from peripheral blood cells and the C677T MTHFR polymorphism was identified by PCR-RFLP. The homocysteine (Hcy) levels were determined by chemiluminescence method. All patients were negative for markers of Wilson's disease, hemochromatosis and autoimmune diseases and have current and past daily alcohol intake less than 100 g/week.ResultsAmong subjects infected with CHC genotype non-1 the frequency of MTHFR genotypes TT was 9.8% versus 4.4% genotype 1 (p = 0.01). Nevertheless, association was found between the MTHFR genotype TT × CT/CC polymorphism and the degree of steatosis and fibrosis in both hepatitis C genotype (p < 0.05). A significant difference was found on plasma Hcy levels in patients with steatosis regardless of HCV genotype (p = 0.03).ConclusionOur results indicate that plasma Hcy levels is highly prevalent in subjects with chronic hepatits C with steatosis regardless of HCV genotype and vitamin deficiency. The presence of genotype TT of MTHFR C677T polymorphism was more common in CHC genotype non-1 infected patient regardless of histopathological classification and genotype TT+CT frequencies were significant in the presence of fibrosis grade 1+2 and of steatosis in CHC infected patients from the northeast of Brazil regardless of HCV genotype. The genetic susceptibility of MTHFR C677T polymorphism should be confirmed in a large population.

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“…MTHFR geninde A1298C polimorfizmi, tek başına plazma homosistein düzeyini anlamlı olarak etkilemezken, C677T varyantı ile birlikteliğinde homosistein düzeyini arttıra-bilir (5). Bir çalışmada asemptomatik yenidoğanlarda, MTHFR C677T geninde homozigot ve heterozigot mutasyon prevalansının %5.3-36.2 olduğu rapor edilmiştir (9). Literatürde hafif düzeyde homosistein yüksekliğiyle beraber heterozigot MTHFR C677T gen mutasyonuyla ilişkili, intrauterin dönem-de sağ bacakta iliofemoral arter trombozu saptanan bir olgu yayınlanmıştır (7).…”

Section: Discussionunclassified