Sperm characteristics in a sample of healthy adolescents in São Paulo, Brazil

Mori, Marcos Mitsuyoshi; Cedenho, A.P.; Koifman, Sérgio; Srougi, Miguel

doi:10.1590/s0102-311x2002000200018

Cited by 6 publications

(7 citation statements)

References 20 publications

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“…We have found only one study performed in healthy adolescents, but it was related to body maturation and classified the individuals according to the Tanner index. Tanner Stage 5 adolescents present higher testicular volume and sperm count than Tanner Stage 4 2. Despite differences in the method of analysis, these data are consistent with our findings of a positive correlation between these parameters.…”

Section: Discussionsupporting

confidence: 91%

“…In addition, biomedical information about the age of first conscious ejaculation (oigarche1), the presence or absence of spermatozoa in seminal fluid, the biological characteristics of spermatozoa during this maturational process, and the time required to reach adult spermatogenesis, is currently scarce and controversial. The few studies reported on this topic correspond to adolescents under the age of 18 years and are related to different pathologies such as testicular cancer 234…”

Section: Introductionmentioning

confidence: 99%

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Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence (12-18 years) and the effect of varicocele

et al. 2016

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This work evaluates sperm head morphometric characteristics in adolescents from 12 to 18 years of age, and the effect of varicocele. Volunteers between 150 and 224 months of age (mean 191, n = 87), who had reached oigarche by 12 years old, were recruited in the area of Barranquilla, Colombia. Morphometric analysis of sperm heads was performed with principal component (PC) and discriminant analysis. Combining seminal fluid and sperm parameters provided five PCs: two related to sperm morphometry, one to sperm motility, and two to seminal fluid components. Discriminant analysis on the morphometric results of varicocele and nonvaricocele groups did not provide a useful classification matrix. Of the semen-related PCs, the most explanatory (40%) was related to sperm motility. Two PCs, including sperm head elongation and size, were sufficient to evaluate sperm morphometric characteristics. Most of the morphometric variables were correlated with age, with an increase in size and decrease in the elongation of the sperm head. For head size, the entire sperm population could be divided into two morphometric subpopulations, SP1 and SP2, which did not change during adolescence. In general, for varicocele individuals, SP1 had larger and more elongated sperm heads than SP2, which had smaller and more elongated heads than in nonvaricocele men. In summary, sperm head morphometry assessed by CASA-Morph and multivariate cluster analysis provides a better comprehension of the ejaculate structure and possibly sperm function. Morphometric analysis provides much more information than data obtained from conventional semen analysis.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence (12-18 years) and the effect of varicocele

et al. 2016

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“…In the first place, although many of these adolescents may present with impaired spermatogenesis [20], not all adolescents with varicocele will present a decrease in fertile potential when they achieve adulthood [10,11]. Furthermore, spermatogenesis is not yet mature in the adolescence, and the alterations observed in a sample may be transient [29]. Therefore, conventional semen analysis is not suitable for a decisive report of the changes in fertile potential caused by varicocele [30].…”

Section: Discussionmentioning

confidence: 99%

Alterations in the proliferative/apoptotic equilibrium in semen of adolescents with varicocele

Belardin

Giudice

Camargo

et al. 2016

J Assist Reprod Genet

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Purpose To verify if the presence of varicocele (grades II and III) with and without seminal alterations, using the 5 th centile cutoff values in table A1.1 of the World Health Organization (WHO, 2010) manual, alters the seminal plasma levels of proteins DNASE1 (deoxyribonuclease-1) and IGFBP7 (Insulin-like growth factor-binding protein 7), which are related to apoptosis regulation and cell proliferation, respectively, demonstrating that these proteins are important for correct spermatogenesis. Methods This cross sectional study was performed at the Sao Paulo Federal University Paulo between May 2014 and April 2016. A total of 61 male adolescents were included in this study, of which 20 controls without varicocele (C), 22 with varicocele and normal semen analysis (VNS) and 19 with varicocele and altered semen analysis (VAS). Seminal plasma from each patient was used for Western blotting analysis of individual protein levels. Values of each protein were normalized to a testicular housekeeping protein (PARK7-protein deglycase DJ-1). Results Levels of IGFBP7 protein are increased in varicocele. Levels of DNASE1 are progressively decreased in varicocele (lower in varicocele and normal semen analysis, lowest in varicocele and altered semen analysis) when compared to adolescents without varicocele. DNASE1 levels are positively correlated with sperm concentration and morphology (correlation values of 0.400 and 0.404, respectively; p values of 0.001 and 0.001, respectively). Conclusion In conclusion, in adolescents, seminal plasma levels of IGFBP7, responsible for proliferative activity, are increased in varicocele grades II and III, and DNASE1, responsible for apoptosis regulation, are lower in varicocele, lowest in varicocele and low semen quality. These proteins demonstrate molecular alterations brought upon by varicocele. Moreover, DNASE1 is capable of discriminating a varicocele that causes alterations to semen quality from one that does not. We propose that the initial response of varicocele is to increase proliferative activity which, if followed by regulation of apoptosis, may lead to the ejaculation of a population of sperm that are in accordance with WHO cutoff values but, in the presence of dysregulated apoptosis, leads to lower sperm concentration and morphology.

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“…These patients and controls could be classified as subfertile concerning the cutoff values for normality in 2 conventional techniques of semen morphology. Moreover, Mori et al (18) studied 127 healthy adolescents (age, 14-20 years) in the city of São Paulo and observed teratozoospermia in 82% of the adolescents who were classified as P5 and G5 according to Tanner's pattern criteria of pubertal changes (13). Likewise, an Italian study evaluated 25 healthy adolescents who were 14 to 18 years old and observed a prevalence of abnormal forms of spermatozoon in 56% (19).…”

Section: Discussionmentioning

confidence: 99%

Minor sperm abnormalities in young male post-pubertal patients with juvenile dermatomyositis

Moraes

Pereira

Cocuzza

et al. 2008

Braz J Med Biol Res

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The objective of the present study was to identify sperm abnormalities in young male patients with juvenile dermatomyositis (JDM). In 2005, 18 male JDM patients, diagnosed according to the criteria of Bohan and Peter, were followed at the Pediatric Rheumatology Unit and Rheumatology Division, of our Institution. Of the 18 males, 11 were pre-pubertal and 7 were postpubertal. Two of 7 post-pubertal JDM male patients were excluded: one for orchidopexy for cryptorchidism and the other for testicular ectopia in the left testis. The remaining 5 post-pubertal JDM patients were prospectively evaluated on the basis of two semen analyses, according to the World Health Organization (WHO), urologic evaluation, testicular Doppler ultrasound hormone profile. The data of the JDM patients were compared with those of 5 age-matched healthy controls. The median age 18, was similar in JDM patients and controls. All JDM patients had teratozoospermia (abnormal sperm morphology), as did 4 (80%) of the controls. One of JDM patients had previous oligoasthenoteratozoospermia treated with intravenous cyclophosphamide with normalization of the number and concentration of the sperm after 5 years. All sperm parameters (sperm concentration, total sperm count and total motile sperm count by WHO, and sperm morphology by Kruger strict criteria), testicular volumes by Prader orchidometer and ultrasound, and hormones were similar in JDM patients compared with controls. The frequency of antisperm antibodies was similar in both groups. All JDM patients had minor sperm abnormalities in the head, midpiece, and/or tail of spermatozoids. Serial semen analyses in larger study populations are necessary to identify the extent and duration of sperm abnormalities in male patients with idiopathic inflammatory myopathies.

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Sperm characteristics in a sample of healthy adolescents in São Paulo, Brazil

Cited by 6 publications

References 20 publications

Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence (12-18 years) and the effect of varicocele

Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence (12-18 years) and the effect of varicocele

Alterations in the proliferative/apoptotic equilibrium in semen of adolescents with varicocele

Minor sperm abnormalities in young male post-pubertal patients with juvenile dermatomyositis

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