Chromosome homogeneity in populations of Triatoma brasiliensis Neiva 1911 (Hemiptera - Reduviidae - Triatominae)

Panzera, Francisco; Pérez, Ruben; Nicolini, P.; Hornos, Sonia; Costa, Jane; Borges, E. C.; Diotaiuti, Liléia; Schofield, Christopher J.

doi:10.1590/s0102-311x2000000800009

Cited by 29 publications

(20 citation statements)

References 13 publications

(9 reference statements)

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“…[8][9][10][11][12] For comparative purposes, the diploid chromosome number (2n) and the quantity of autosomal heterochromatin for each species are included in Table 1 (F. Panzera and others, unpublished data). 9,10,[15][16][17] …”

Section: Resultsmentioning

confidence: 99%

Genome Size Determination in Chagas Disease Transmitting Bugs (Hemiptera-Triatominae) by Flow Cytometry

Panzera

Ferrandis²,

Ramsey³

et al. 2007

The American Journal of Tropical Medicine and Hygiene

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Abstract. Because information about genome size in triatomines is scarce and contradictory, we performed DNA quantification by flow cytometry in 13 species belonging to five genera (Dipetalogaster, Eratyrus, Panstrongylus, Rhodnius, and Triatoma) to infer overall tendencies and phylogenetic associations. The results show that the haploid DNA content of the subfamily Triatominae varies nearly 4-fold, from < 0.7 pg in Rhodnius species (0.6 × 10 9 bp) to 2.7 pg in Triatoma delpontei (2.6 × 10 9 bp). Considering that triatomines present similar chromosome numbers, we suggest that genome size differences are the result of variation in the quantity of repetitive DNA sequences localized in hetero and euchromatin. Changes in heterochromatin are particularly important when considering populations or closely related species; in more distant taxa, euchromatic changes also play a role. Our analyses indicate that flow cytometry is a useful tool for population, taxonomic, and evolutionary studies in this subfamily.

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Section: Resultsmentioning

confidence: 99%

Genome Size Determination in Chagas Disease Transmitting Bugs (Hemiptera-Triatominae) by Flow Cytometry

Panzera

Ferrandis²,

Ramsey³

et al. 2007

The American Journal of Tropical Medicine and Hygiene

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“…There was no variation in the distribution of heterochromatin blocks between P. torridus individuals displaying different color patterns. The absence of intraspecific variation was also observed for different populations of T. brasiliensis (Panzera et al, 2000), R. neglectus (Alevi et al, 2015a), and P. megistus (Alevi et al, 2015b).…”

Section: Discussionmentioning

confidence: 90%

Distribution of constitutive heterochromatin in Pachycoris torridus (Hemiptera, Scutelleridae) with different chromatic patterns

Souza-Firmino

Alevi²,

Pereira

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The stink bug Pachycoris torridus is a pest of great agricultural importance due to its records on culture of physic nut (Jatropha curcas), which is the raw material for biodiesel production. An interesting feature of this insect is its high phenotypic variability, a characteristic that resulted in it being classified as a new species on eight separate occasions. In the suborder Heteroptera, the heterochromatin pattern is specific and often allows species to be differentiated. To confirm whether there is differentiation between specimens of P. torridus with different color patterns (yellow, orange, brown, and red), samples were analyzed cytogenetically using the C-banding method. During meiotic prophase, the four color patterns analyzed showed a large heterochromatic 15750 T.S. Souza-Firmino et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15749-15753 (2015) chromocenter, consisting of a combination of both sex chromosomes (X and Y). Thus, the present study reports chromosomal homogeneity in different color patterns of P. torridus and highlights the importance of this tool in the description of new species.

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“…Considering that T. lenti and T. petrochiae have the same cytogenetic characteristics described for the Brasiliensis subcomplex (Panzera et al, 2000;Alevi et al, 2012bAlevi et al, , 2013bAlevi et al, , 2014a, it is possible that this mutation in haplotype 4 may be an initial molecular tool that supports inclusion of these species within the subcomplex.…”

Section: Discussionmentioning

confidence: 91%

“…All species of the Brasiliensis subcomplex have cytogenetic synapomorphies: 20 autosomes plus two sex chromosomes (XY in males and XX in females), C-blocks in one or both chromosomal ends in all autosomal pairs, a large chromocenter made up of both sex chromosomes plus two autosomal pairs, and multiple C-dots spread in the nucleus during early meiotic prophase (Panzera et al, 2000;Alevi et al, 2013aAlevi et al, , 2014a. T. vitticeps, T. melanocephala, and T. tibiamaculata, which were excluded from this subcomplex, have been found to have multiple sex systems and different C-heterochromatin patterns (Panzera et al, 2010;Alevi et al, 2012aAlevi et al, , 2013aAlevi et al, , 2014b.…”

Section: Discussionmentioning

confidence: 99%

Insights into a hotspot in the Brasiliensis subcomplex (Hemiptera, Triatominae) by analysis of D2 domain of the nuclear gene 28S

Guerra¹,

Alevi²,

Banho³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The Brasiliensis subcomplex is a monophyletic group formed by the species Triatoma brasiliensis brasiliensis, T. b. macromelasoma, T. juazeirensis, T. melanica, and T. sherlocki. However, using cytogenetic data and experimental hybrid crosses, T. lenti and T. petrochiae were also grouped into this subcomplex. This study aims to analyze the properties of hotspot in the D2 domain of the nuclear gene 28S in all species of the Brasiliensis subcomplex as well as T. lenti and T. petrochiae. These species show two transversions at position 385 (G↔C and T↔G). We suggest that this mutation in haplotype 4 may be an initial molecular tool that supports the relationship of these species with the subcomplex. In addition to the transversion at haplotype 4, these species, aside from T. melanica, also possess a transversion at position 385 (G↔T) in haplotype 1. Thus, we describe the hotspot mutations of the D2 domain of the nuclear gene 28S for species in Brasiliensis subcomplex as follows: three transversions are present at position 385 of haplotypes 1 and 4, which are shared by members of the subcomplex as well as T. lenti and T. petrochiae. These transversions may be considered a synapomorphy between these species. However, we emphasize that new phylogenetic studies should be conducted to evaluate whether T. lenti and T. petrochiae are truly members of the Brasiliensis subcomplex.

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Chromosome homogeneity in populations of Triatoma brasiliensis Neiva 1911 (Hemiptera - Reduviidae - Triatominae)

Abstract: Triatoma brasiliensis is the most important vector of Chagas disease in the semiarid

Cited by 29 publications

References 13 publications

Genome Size Determination in Chagas Disease Transmitting Bugs (Hemiptera-Triatominae) by Flow Cytometry

Genome Size Determination in Chagas Disease Transmitting Bugs (Hemiptera-Triatominae) by Flow Cytometry

Distribution of constitutive heterochromatin in Pachycoris torridus (Hemiptera, Scutelleridae) with different chromatic patterns

Insights into a hotspot in the Brasiliensis subcomplex (Hemiptera, Triatominae) by analysis of D2 domain of the nuclear gene 28S

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