Molecular epidemiology of dengue viruses in Brazil

Nogueira, Rita Maria Ribeiro; Miagostovich, Marize Pereira; Schatzmayr, Hermann G.

doi:10.1590/s0102-311x2000000100021

Cited by 11 publications

(9 citation statements)

References 41 publications

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“…The diagnosis of DENV infection was confirmed by anti‐dengue enzyme‐linked immunosorbent assay (ELISA) IgM, 20 serotype‐specific reverse transcription–polymerase chain reaction (RT‐PCR) or by virus isolation 21 . The immune response to dengue was considered as primary or secondary by IgG ELISA, according to previously established criteria 22 …”

Section: Methodsmentioning

confidence: 99%

Differential regulation of toll‐like receptor‐2, toll‐like receptor‐4, CD16 and human leucocyte antigen‐DR on peripheral blood monocytes during mild and severe dengue fever

et al. 2010

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SummaryDengue fever (DF), a public health problem in tropical countries, may present severe clinical manifestations as result of increased vascular permeability and coagulation disorders. Dengue virus (DENV), detected in peripheral monocytes during acute disease and in in vitro infection, leads to cytokine production, indicating that virus-target cell interactions are relevant to pathogenesis. Here we investigated the in vitro and in vivo activation of human peripheral monocytes after DENV infection. ing that these cells might be involved in controlling exacerbated inflammatory responses, probably by IL-10 production. We showed here, for the first time, phenotypic changes on peripheral monocytes that were characteristic of cell activation. A sequential monocyte-activation model is proposed in which DENV infection triggers TLR2/4 expression and inflammatory cytokine production, leading eventually to haemorrhagic manifestations, thrombocytopenia, coagulation disorders, plasmatic leakage and shock development, but may also produce factors that act in order to control both intense immunoactivation and virus replication.

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Section: Methodsmentioning

confidence: 99%

Differential regulation of toll‐like receptor‐2, toll‐like receptor‐4, CD16 and human leucocyte antigen‐DR on peripheral blood monocytes during mild and severe dengue fever

et al. 2010

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“…Probably as a result of this, the Brazilian DENV-1 population has a relatively low degree of genetic diversity. Given that DENV-1 is now known to be evolving at a rate of approximately *7 9 10 -4 substitutions per site per year in the E gene [42], it is apparent that the low diversity DENV-1 population in Brazil was probably only founded at some time in the early to mid-1980s: approximately at the time when the first DENV-1 cases were reported in Brazil [43,44]. While it is possible that sufficient geographical structure might be detectable within Brazilian DENV-1 populations to trace the movements across the country of epidemic DENV-1 variants using phylogeographic analyses, the low degrees Brazilian DENV-1 diversity would require the sampling and sequencing of substantially more full-length genomes than are currently available.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Comparative analysis of American Dengue virus type 1 full-genome sequences

et al. 2009

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Dengue virus (DENV; Genus Flavivirus, Family Flaviviridae) has been circulating in Brazil since at least the mid-1980s and continues to be responsible for sporadic cases of Dengue fever and Dengue hemorrhagic fever throughout this country. Here, we describe the full genomes of two new Brazilian DENV-serotype 1 (DENV-1) variants and analyze these together with all other available American DENV-1 full-genome sequences. Besides confirming the existence of various country-specific DENV-1 founder effects that have produced a high degree of geographical structure in the American DENV-1 population, we also identify that one of the new viruses is one of only three detectable intra-American DENV-1 recombinants. Although such obvious evidence of genetic exchange among epidemiologically unlinked Latin American DENV-1 sequences is relatively rare, we find that at the population-scale there exists substantial evidence of pervasive recombination that most likely occurs between viruses that are so genetically similar that it is not possible to reliably distinguish and characterize individual recombination events.

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“…Based on serological analysis, DENV can be differentiated as four distinct serotypes (DENV-1, DENV-2, DENV-3 and DENV-4), each one with the capacity to infect and cause even the more severe manifestation, although some serotypes have been isolated more frequently in DHF epidemics. On the other hand, evolution studies and molecular epidemiology using nucleotide sequences from the DENV genome have demonstrated the occurrence of genotype clades within each serotype [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28]. For this reason, genetic characterization of DENV has become a critical issue for understanding epidemic patterns of viral spread.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia

Méndez

Usme-Ciro

Domingo

et al. 2010

Virol J

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BackgroundDengue Fever is one of the most important viral re-emergent diseases affecting about 50 million people around the world especially in tropical and sub-tropical countries. In Colombia, the virus was first detected in the earliest 70's when the disease became a major public health concern. Since then, all four serotypes of the virus have been reported. Although most of the huge outbreaks reported in this country have involved dengue virus serotype 1 (DENV-1), there are not studies about its origin, genetic diversity and distribution.ResultsWe used 224 bp corresponding to the carboxyl terminus of envelope (E) gene from 74 Colombian isolates in order to reconstruct phylogenetic relationships and to estimate time divergences. Analyzed DENV-1 Colombian isolates belonged to the formerly defined genotype V. Only one virus isolate was clasified in the genotype I, likely representing a sole introduction that did not spread. The oldest strains were closely related to those detected for the first time in America in 1977 from the Caribbean and were detected for two years until their disappearance about six years later. Around 1987, a split up generated 2 lineages that have been evolving separately, although not major aminoacid changes in the analyzed region were found.ConclusionDENV-1 has been circulating since 1978 in Colombia. Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Viral strains used in the present study did not form a monophyletic group, which is evidence of a polyphyletic origin. We report the rapid spread patterns and high evolution rate of the different DENV-1 lineages.

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Molecular epidemiology of dengue viruses in Brazil

Abstract: Dengue viruses (DEN) are found as four antigenically distinct serotypes designated 2, 3, and 4

Cited by 11 publications

References 41 publications

Differential regulation of toll‐like receptor‐2, toll‐like receptor‐4, CD16 and human leucocyte antigen‐DR on peripheral blood monocytes during mild and severe dengue fever

Differential regulation of toll‐like receptor‐2, toll‐like receptor‐4, CD16 and human leucocyte antigen‐DR on peripheral blood monocytes during mild and severe dengue fever

Comparative analysis of American Dengue virus type 1 full-genome sequences

Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia

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