Evaluation of chromium concentration in cattle feces using different acid digestion and spectrophotometric quantification techniques

Souza, N.K.P.; Detmann, Edênio; Piña, D. S.; Filho, Sebastião de Campos Valadares; Sampaio, Cláudia Batista; Queiróz, Augusto César de; Veloso, Cristina Mattos

doi:10.1590/s0102-09352013000500028

Cited by 19 publications

(15 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fecal samples were analyzed for chromium oxide using an atomic absorption spectrophotometer, according to the methodology described by Souza et al [22].…”

Section: Pasture and Concentrate Intakementioning

confidence: 99%

Feeding Protected Lysine and Methionine Modifies Milk Protein Profile in Grazing Dairy Cows

Quintero¹,

Olivera-Angel²

2019

View full text Add to dashboard Cite

The experiment was designed to determine the effect of protected lysine (Lys) and methionine (Met) supply on milk protein profile in grazing dairy cows specifically in the caseins (CNs) and α-lactalbumin fractions. Twelve multiparous mid lactation Holstein cows producing 24 (±4.76) kg of milk were assigned to one of two treatments (six cows per treatment) during an experimental period of 21 days. In the control (C) group, cows grazed a Pennisetum clandestinum pasture and were supplemented with a commercial concentrate according to milk production. In the Met-Lys treatment, cows received the same ration supplemented with protected Lys and Met. Milk yield and composition and milk protein profile were measured at the start and the end (21 st day) of the experimental period. The Tricine-SDS-PAGE and the Gel-Quant Express Analysis (Invitrogen) software were used to determine milk protein composition. Statistical analysis was performed using the SAS's PROC MIXED procedure through a mixed model that included the animal as a random effect and the treatments as a fixed effect adjusted by covariables. Milk production averaged 23.7 (±2.0) kg cow −1 day −1 without differences between treatments (P < 0.96). Yield of fat corrected milk (4% FCM) tended (P < 0.10) to increase in the Met-Lys treatment (26.0 kg cow −1 day −1) compared to C (24.2 kg cow −1 day −1). Milk protein content (g/kg) did not differ (C = 30.4; Met-Lys = 31.1) and lactose content tended (P < 0.08) to be higher in the Met-Lys (47.4) group compared to C (45.9). Milk protein content (g/kg) of αS1-CN resulted higher (P < 0.046) in Met-Lys (10.58) compared to C (9.44). Concentration of β-CN also increased (P < 0.05) after protected aminoacid supply (C = 9.58; Met-Lys = 10.35). It can be concluded that milk protein composition was improved by protected Lys-Met supply without altering other compositional parameters of milk composition. Milk nutritional quality and its potential yield for cheese-making were positively enhanced.

show abstract

“…Fecal samples were analyzed for chromium oxide using an atomic absorption spectrophotometer, according to the methodology described by Souza et al [22].…”

Section: Pasture and Concentrate Intakementioning

confidence: 99%

Feeding Protected Lysine and Methionine Modifies Milk Protein Profile in Grazing Dairy Cows

Quintero¹,

Olivera-Angel²

2019

View full text Add to dashboard Cite

show abstract

“…These were stored in plastic labeled jars, and subsequently analyzed for the chromium contents using nitric-perchloric digestion and atomic absorption spectrometry (SOUZA et al, 2013); titanium dioxide, by colorimetry (TITGEMEYER et al, 2001); and DM, CP, EE, NDFap, iNDF and MM, as described previously.…”

Section: Nfc = 100 -[(%Cp − %Cp From Urea + % Urea) + %Ndfap + %Ee + mentioning

confidence: 99%

“…The effect of supplementation and the linear and quadratic effects of the level of substitution of soybean meal for cottonseed meal were evaluated by decomposing the sum of squares of treatments using orthogonal contrasts (STEEL et al, 1997). All procedures were conducted using PROC MIXED from SAS (version 9.2).…”

Section: Dmi (Kg/day) = {[(Fdm×cmf)-ms]/cmfo} + Sdmimentioning

confidence: 99%

Substitution of soybean meal for cottonseed meal in multiple supplements for grazing beef heifers in the dry season

et al. 2016

View full text Add to dashboard Cite

The objective of this study was to evaluate the effect of substituting soybean meal for cottonseed meal in multiple supplements on the nutritional characteristics and performance of beef heifers in their postweaning phase on Brachiaria decumbens pastures during the dry season. Twenty-four Nellore beef heifers (average initial age and weight of 8 mo and 210±6 kg, respectively) were used. The design was completely randomized, with four treatments and six replicates. Supplements contained approximately 30% crude protein (CP) and a progressive substitution of soybean meal for cottonseed meal (0, 50 and 100%). The control animals received only a mineral mixture ad libitum, and those on the other treatments received supplementation at 1.0 kg/animal/day. No differences were found in ADG between supplemented and control animals (P>0.10). Supplementation increased crude protein (CP) intake only (P<0.10). The level of substitution of soybean meal for cottonseed meal did not affect (P>0.10) the intake of supplemented animals. Supplementation elevated the apparent digestibility coefficients (P<0.10) of OM, CP, NFC and TDN, but not EE or NDFap (P>0.10). A positive linear effect (P<0.10) of the level of substitution of soybean meal for cottonseed cake was observed on the digestibility of OM, NFC and TDN. Supplementation and the level of substitution had an effect (P<0.10) on the serum urea nitrogen and urine urea nitrogen contents. Supplementation or substitution level had no effect on the flow of microbial nitrogen to the intestine (MICN) or efficiency of microbial protein synthesis (EMPS) (P>0.10). Substitution caused a decreasing linear effect (P<0.10) on microbial nitrogen/nitrogen intake ratio (MICNR). In conclusion, substitution of soybean meal for cottonseed meal in multiple supplements during the dry season does not impair the productive performance of beef heifers. Key words: Braquiaria decumbens, digestibility, intake, supplementation ResumoObjetivou-se avaliar o efeito da substituição do farelo de soja pelo farelo de algodão em suplementos múltiplos sobre as características nutricionais e desempenho produtivo de novilhas de corte em fase de recria em pastagens de Brachiaria decumbens no período da seca. Foram utilizadas 24 novilhas de corte nelore com idade e peso médio inicial de 8 meses e 210±6 kg, respectivamente. O delineamento foi inteiramente casualizado com quatro tratamentos e seis repetições. Os suplementos continham aproximadamente 30% de proteína bruta (PB) e substituição progressiva do farelo de soja pelo farelo de algodão em 0, 50 e 100%. Aos animais do tratamento controle foi fornecida apenas mistura mineral ad libitum e aos demais tratamentos foram fornecidos 1,0 kg/animal/dia de suplemento. Não houve diferença de GMD entre os animais suplementados e os animais controle (P>0,10). A suplementação aumentou apenas o consumo de proteína bruta (PB) (P<0,10). O nível de substituição de farelo de soja pelo farelo de algodão não afetou (P>0,10) o consumo nos animais suplementados. A suplementação amp...

show abstract

“…Samples of feces were analyzed for the concentration of chromium and titanium dioxide using nitric-perchloric digestion and atomic absorption spectrometry (SOUZA et al, 2013) and colorimetry, respectively (DETMANN et al, 2012).…”

Section: Chemical Analysesmentioning

confidence: 99%

Quantidades de suplementos para novilhas de corte sob pastejo durante o período de transição seca - águas

et al. 2016

View full text Add to dashboard Cite

The objective of this study was to evaluate the effect of supplying different quantities of multiple supplements on the nutritional characteristics and productive performance of heifers during the postweaning phase on Urochloa decumbens pastures during the dry-rainy transition season. Twentyfour heifers (average initial age and weight of 11 mo and 243±6 kg, respectively) were used. The experimental design was completely randomized, with four treatments and six replicates. A supplement containing 25% CP was used, and treatments consisted of the following four levels of supplements: 0, 0.5, 1.0 and 1.5 kg. The animals in the different treatments received a mineral mixture ad libitum. A positive linear effect (P<0.10) was observed on ADG and final body weight with the quantities of supplements. An increasing linear effect (P<0.10) was found on the intakes of DM, OM, CP, EE, NFC and TDN with the elevation in the amount of the supplement provided, which was not observed for NDFap intake. A cubic effect was observed (P<0.10) on the apparent digestibility of OM, CP, NDFap, NFC and concentration of TDN among the quantities of multiple supplements. Supplement levels increased (P<0.10) the digestibility coefficient of EE. An increasing linear response was detected (P<0.10) in urine urea nitrogen excretion, serum urea nitrogen (SUN) and flow of microbial nitrogen (MICN) compounds with the quantities of supplement. No difference was observed (P<0.10) in the efficiency of microbial synthesis between the supplementation levels. The quantities of supplement had a decreasing linear effect (P<0.10) on the relative MICN. In conclusion, supplying higher amounts of multiple supplements during the dry-rainy transition season improves the productive performance and nutritional characteristics of beef heifers reared on pasture. Key words: Digestibility, intake, supplementation, post-weaning, Urochloa decumbens ResumoO objetivo do trabalho foi avaliar o efeito do fornecimento de diferentes quantidades de suplementos múltiplos sobre as características nutricionais e desempenho produtivo de novilhas durante a fase de recria em pastagens com Urochloa decumbens no período de transição seca-águas. Foram utilizadas 24 novilhas com idade e peso médio inicial de 11 meses e 243±3 kg, respectivamente. O delineamento foi inteiramente casualizado com quatro tratamentos e seis repetições. Utilizou-se um suplemento com 25% de PB. Os tratamentos consistiam em quatro quantidades de suplementos: 0; 0,5; 1,0 e 1,5 kg respectivamente. Os animais dos diferentes tratamentos receberam mistura mineral ad libitum. Observou-se efeito linear positivo (P<0,10) no GMD e peso corporal final (PCF) com as quantidades de suplementos. Foi verificado efeito linear crescente (P<0,10) no consumo de MS, MO, PB, EE, CNF e NDT com o fornecimento de maiores quantidades de suplemento; efeito não apresentado no consumo de FDNcp. Evidenciou-se efeito cúbico (P<0,10) sobre a digestibilidade aparente da MO, PB, FDNcp, CNF e concentração de NDT entre as quantidades de supl...

show abstract

Evaluation of chromium concentration in cattle feces using different acid digestion and spectrophotometric quantification techniques

Cited by 19 publications

References 12 publications

Feeding Protected Lysine and Methionine Modifies Milk Protein Profile in Grazing Dairy Cows

Feeding Protected Lysine and Methionine Modifies Milk Protein Profile in Grazing Dairy Cows

Substitution of soybean meal for cottonseed meal in multiple supplements for grazing beef heifers in the dry season

Quantidades de suplementos para novilhas de corte sob pastejo durante o período de transição seca - águas

Contact Info

Product

Resources

About