Prevalence of hepatitis A virus in sea food in Iran

Momtaz, Hassan; Rahimi, Ebrahim; Hajian, Sam; Momeni, Mohammad

doi:10.1590/s0102-09352012000400015

Cited by 2 publications

(3 citation statements)

References 19 publications

(12 reference statements)

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“…The total incidence of HAV amongst shellfish samples was 1.33%. Lower incidence rates of HAV in shellfish samples were reported in Italy (0.9%) (11) and South Korea (0.7%) (12), while higher incidence rates were observed in Iran (2.70%) (13) and Portugal (33%) (14). The total incidence of NoV GI amongst shellfish samples was 8.00%.…”

Section: Discussionmentioning

confidence: 92%

Presence of Enteric Viruses in Shellfish Samples from the Persian Gulf

2023

ARI

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Shellfishes are significant economic and nutritious seafood amongst people of different countries. Seafood products, particularly shellfish samples, are potential reservoirs of enteric viruses. This research investigated the incidence of RoV, NoV GI and GII, HAV, and HEV in shellfish samples from the Persian Gulf, Iran. One hundred and fifty shellfish samples were collected. RNA extraction and cDNA synthesis was performed using commercial kits. The real-time PCR assessed the presence of enteric viruses in extracted cDNA samples. Thirty-two out of 150 (21.33%) shellfish samples were contaminated with enteric viruses. Prevalence of NoV GI, NoV GII, HAV, and RoV amongst shellfish samples were 8.00%, 11.33%, 1.33%, and 0.66%, respectively. There were no contaminated shellfish samples with HEV. Simultaneous prevalence of HAV and NoV GI and HAV and NoV GII viruses were 0.66% and 0.66%, respectively. Examined viruses had a higher prevalence in shellfish samples collected through the winter season (P < 0.05). Prevalence of HAV, RoV, NoV GI, and NoV GII amongst shellfish samples collected through the winter season was 2.85%, 9.09%, 11.90%, and 20%, respectively. To the best of our knowledge, this is the first report of the incidence of enteric viruses, particularly HAV, NoV GI, NoV GII, and RoV, in shellfish samples from the Persian Gulf, Iran. Shellfish samples may serve as a potential source of enteric viruses for the human population. Therefore, routine viral assessments should be conducted. Completely cooking shellfish samples before consumption can significantly reduce the risk of HAV, RoV, NoV GI, and NoV GII infections. Furthermore, given the export value and importance of shellfish samples, their microbial quality and safety should be routinely monitored.

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Section: Discussionmentioning

confidence: 92%

Presence of Enteric Viruses in Shellfish Samples from the Persian Gulf

2023

ARI

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“…In accordance with these results, some studies have previously shown that S. aureus isolates may be negative for the coa gene. Momtaz et al reported that only 42 of 86 S. aureus isolates in milk samples from bovine mastitis cases contained the coa gene in PCR-RFLP analysis (17). In study of Karahan and Cetinkaya, the coa gene was detected in 80.5% of S. aureus strains isolated from subclinical cases of mastitis (14).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, they reported different coa-RFLP patterns among S. aureus isolates from goat, sheep and bovine milk samples(22). Momtaz et al reported the presence of certain coa genotypes of S. aureus in cases of mastitic in dairy herds in Isfahan, Iran(17).…”

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confidence: 99%

Genotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis

et al. 2017

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Background and Objectives: Determining the genetic relationship between S. aureus isolates is important for epidemiological surveillance and control of infections caused by this bacterium. The present study was conducted to determine polymorphisms of coagulase gene (coa) among S. aureus isolates from pastry and cheese samples using restriction fragment length polymorphism (RFLP) analysis. Methods: Overall, 65 S. aureus isolated from pastry (n=45) and cheese (n=20) samples were examined for the coa gene by polymerase chain reaction (PCR). PCR products were digested with AluI enzyme and the products were assessed using gel electrophoresis. Results: Except for two isolates, all isolates were positive in coa-PCR and produced four different PCR products, with molecular sizes ranging from 570 to 970 bp. Overall; five distinct RFLP patterns were detected (I-V). Although pattern types I and III were present in isolates from both samples, types I and IV were mainly present in isolates from cheese and pastry samples, respectively. Conclusion: PCR-RFLP analysis of the coa gene indicates that S. aureus isolates from pastry and cheese samples may be originated from different sources. However, as one pattern type was predominant in each group, it can be concluded that majority of the isolates may have the same origin.

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Prevalence of hepatitis A virus in sea food in Iran

Cited by 2 publications

References 19 publications

Presence of Enteric Viruses in Shellfish Samples from the Persian Gulf

Presence of Enteric Viruses in Shellfish Samples from the Persian Gulf

Genotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis

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