Semen collection and evaluation of captive coatis (Nasua nasua)

Paz, Regina Celia Rodrigues da; Morgado, Thais Oliveira; Viana, Cabral; Arruda, F.P.; Nascimento, D.O. Bezerra do; Guimarães, Lia Buarque de Macedo

doi:10.1590/s0102-09352012000200010

Cited by 4 publications

(5 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of the sperm parameters found in the present study may differ from those already described for coatis Lima et al 2009;Queiroz et al 2010;Paz et al 2012a,b), such as sperm concentration, total motility and vigour, showing that different individuals of the same species present heterogeneity in these parameters. The percentage of viable spermatozoa with intact membranes was higher than 70%, as described in other studies Lima et al 2009;Queiroz et al 2010;Paz et al 2012a). The differences found in some of these evaluated parameters may be related to the collection technique, anaesthetic procedure used (Silva et al 2004) and the interference of factors related to genetics, environment and nutrition (Morato et al 2001).…”

Section: Discussionsupporting

confidence: 79%

“…; Paz et al. ). The differences found in some of these evaluated parameters may be related to the collection technique, anaesthetic procedure used (Silva et al.…”

Section: Discussionmentioning

confidence: 97%

“…; Paz et al. ,b), such as sperm concentration, total motility and vigour, showing that different individuals of the same species present heterogeneity in these parameters. The percentage of viable spermatozoa with intact membranes was higher than 70%, as described in other studies (Barros et al.…”

Section: Discussionmentioning

confidence: 98%

See 2 more Smart Citations

Morphometry, Morphology and Ultrastructure of Ring‐tailed Coati Sperm (Nasua nasua Linnaeus, 1766)

Silva

Magalhães

Ribeiro³

et al. 2015

Reprod Domestic Animals

View full text Add to dashboard Cite

The ring-tailed coati (Nasua nasua) is a procyonid whose population is in sharp decline. Therefore, studies are needed to better understand the reproduction of this animal. For this reason, this study aimed to evaluate the morphology, morphometry and sperm ultrastructure of ring-tailed coati sperm. Four captive adult males were used for this study. Slides stained with Bengal Rose were used for the morphometric and morphologic analyses. The length and width of the head were measured, as well as the length of the midpiece and tail and the total length of the sperm. Scanning electron microscopy and transmission electron microscopy were used for the ultrastructural analyses. The most obvious morphological abnormalities observed were coiled tails (6.1 ± 8.7%) and the lack of acrosomes (5.4 ± 4.4%). Regarding the morphometry, the measurements of the head (length × width), midpiece (length) and tail (length) were (mean ± SD) 6.2 ± 0.4 × 8.1 ± 0.6 μm, 14.1 ± 0.5 and 63.9 ± 4.1 μm, respectively, and the total length of the sperm was 86.1 ± 4.3 μm. Through electron microscopy, the presence of electron-lucent points in the nucleus and the presence of approximately 55 mitochondrial spirals in the midpiece were identified. The data obtained in this study provide detailed information on the sperm characteristics of coatis and may inform future research on germplasm conservation, both for this species and other threatened procyonids.

show abstract

Section: Discussionsupporting

confidence: 79%

“…; Paz et al. ). The differences found in some of these evaluated parameters may be related to the collection technique, anaesthetic procedure used (Silva et al.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Morphometry, Morphology and Ultrastructure of Ring‐tailed Coati Sperm (Nasua nasua Linnaeus, 1766)

Silva

Magalhães

Ribeiro³

et al. 2015

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…Extenders based on TRIS plus egg yolk and glycerol were reported for collared peccary semen cryopreservation [17]. Other compounds have been described, such as the INRA ® extender for tapir [13], the lactose-egg yolk extender for jaguars [28], and a combination of TES and TRIS extenders for capuchin monkey [16]. Recently, a coconut water-based extender was reported for semen cryopreservation in collared peccaries [29], agoutis [30], and squirrel monkeys (Saimiri collinsi) [31].…”

Section: Insights From Animal Reproductionmentioning

confidence: 99%

Assisted Reproductive Techniques on South American Wild Mammals

Silva¹,

Pereira²,

Lima³

et al. 2016

Insights From Animal Reproduction

View full text Add to dashboard Cite

For South American wild mammals, assisted reproductive techniques (ARTs) have been established in accordance with the procedures used for domestic mammals. However, often, it is not possible to infer the experimental conditions from one species to another. In general, the use of these ARTs aims the conservation of the animal genetic material, using gamete manipulation, embryo technology by in vivo or in vitro methods, and cloning. In all animals, the previous knowledge of reproductive physiology becomes the starting point for the use of ARTs in the expansion of all species of interest. In this sense, the purpose of this chapter is to develop an approach of theoretical, technical, and applied aspects of the ARTs in South American wild mammals, with emphasis on the state-of-theart and its progress and perspectives.

show abstract

“…The distinct seasonal changes observed in these parameters indicated the highest values occurring during the dry season (winter) and coinciding with the breeding season for coatis in this geographic location. However, no seasonal differences in the production of both, functionally intact and morphologically normal sperm have been reported (PAZ et al, 2012b). According to Beisiegel (2001), the birthing period in Nasua nasua occurs between October and November in southeastern Brazil.…”

Section: Introductionmentioning

confidence: 99%

Coatis (Nasua nasua) semen cryopreservation

Paz

Avila

2015

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

Carnivore semen cryopreservation procedures started with semen washing and centrifuging in culture media for seminal plasma removal and microorganisms elimination. The objective of this study was to perform coatis semen cryopreservation comparing the effects between two extenders Ham's F-10 and M199 for washing and centrifugation before cryopreservation using Dilutris medium. Semen samples (n = 36) were collected by electroejaculation from six adult male coatis (Nasua nasua) between May and October of 2008 at the Universidade Federal de Mato Grosso Zoo. Sperm total motility (%), progressive sperm motility (0-5), plasma membrane integrity spermatozoa rates (%), and acrosome integrity (%) were analyzed. These fresh semen samples were divided in two fractions, diluted in 1 ml of Ham's F-10 (Ham's F-10, Nutricel S.A., Brazil) or M199 (M199, Nutricel S.A., Brazil) and centrifuged at 300 g for 10 min. The supernatant was discarded and pellets resuspended in 1 ml of Dilutris (Dilutris, Minitube ® , Brazil), stored at 5ºC for 3 hours, transferred to 0.25 ml straws, placed in liquid nitrogen vapor for 20 min, and immersed in liquid nitrogen. The means/SD for fresh semen and cryopreserved semen using Ham's F-10/Dilutris and M199/Dilutris were, respectively: 84.28 ± 11.57, 45.38 ± 27.26, and 44.61 ± 25.03 for total motility; 3.64 ± 1.44, 2.15 ± 1.14, and 2.07 ± 1.03 for progressive sperm motility; 92.76 ± 3.46, 84.69 ± 15.77, and 89.76 ± 13.97 for live spermatozoa rate; and 94.76 ± 2.89, 92.35 ± 4.73, and 90.58 ± 7.17 for acrosome integrity. No significant difference (P < 0.05) were observed between the values obtained from the Ham's F-10/Dilutris or M199/Dilutris treatments. Both treatments demonstrated to be suitable for freezing semen from this species.Keywords: Procyonidae. Carnivore. Semen. Cryopreservation. Reproduction. ResumoOs procedimentos de criopreservação do sêmen em carnívoros devem ser iniciados após a lavagem e centrifugação em meios de cultura para retirada do plasma seminal e eliminação de microrganismos. O objetivo deste estudo foi realizar a criopreservação do sêmen de quatis comparando os efeitos entre dois extensores Ham's F-10 e M199 para lavagem e centrifugação do sêmen antes do congelamento com o meio Dilutris. Amostras de sêmen (n = 36) foram coletadas por eletroejaculação em seis quatis (Nasua nasua) machos adultos entre maio e outubro de 2008, no Zoológico da Universidade Federal de Mato Grosso. Motilidade total (%), motilidade espermática progressiva (0-5), integridade da membrana plasmática (%) e integridade do acrossoma (%) foram analisados. Amostras de sêmen a fresco foram divididas em duas frações, diluídas em 1 mL de Ham's F-10 (Ham's F-10, Nutricel S.A., Brasil) ou M199 (M199, Nutricel S.A., Brazil) e centrifugado a 300 g por 10 min. O sobrenadante foi descartado e pellets ressuspendidos em 1 mL de Dilutris (Dilutris, Minitube ® , Brasil), armazenados a 5ºC durante 3 horas, transferidos para palhetas de 0,25 mL, colocadas em vapor de nitrogênio líquido por 20 min e imersos em nitr...

show abstract

Semen collection and evaluation of captive coatis (Nasua nasua)

Cited by 4 publications

References 11 publications

Morphometry, Morphology and Ultrastructure of Ring‐tailed Coati Sperm (Nasua nasua Linnaeus, 1766)

Morphometry, Morphology and Ultrastructure of Ring‐tailed Coati Sperm (Nasua nasua Linnaeus, 1766)

Assisted Reproductive Techniques on South American Wild Mammals

Coatis (Nasua nasua) semen cryopreservation

Contact Info

Product

Resources

About