Phylogenetic analysis of Pneumocystis from pig lungs obtained from slaughterhouses in southern and midwestern regions of Brazil

Sanches, Edna Maria Cavallini; Ferreiro, Laerte; Borba, Mauro Riegert; Spanamberg, Andréia; Ravazzolo, Ana Paula; Santurio, Jânio M.; D., Driemeir,; Barcellos, Daphne Câmara; Berthelemy, Madeleine; Guillot, Jacques

doi:10.1590/s0102-09352011000500016

Cited by 6 publications

(13 citation statements)

References 19 publications

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“…Alignment of those pig derived sequences to another porcine sequence assigned to the mtLSU published in the same paper (Sanches et al. ) revealed a sequence homology of up to 95.2%. In contrast, alignment to sequences of the 16S rRNA resulted in noticeable lower percentages of homology, although the fact, that none of the available sequences was a porcine one, was considered.…”

Section: Discussionmentioning

confidence: 90%

“…suis mtLSU sequence published in the same article using the same primers (Sanches et al. ). BLAST analysis of acc.…”

Section: Resultsmentioning

confidence: 99%

“…The primers that have been used for sequencing are declared, but they are also typical for mitochondrial rRNA gene sequences. Alignment of those pig derived sequences to another porcine sequence assigned to the mtLSU published in the same paper (Sanches et al 2011)…”

Section: Discussionmentioning

confidence: 99%

“…no. KC494265-KC494279 are assigned to the 16S rRNA in GenBank (Sanches et al 2011). Also the primer sequences are published, but they are designed for detection of mtLSU sequences.…”

Section: The Nuclear Ribosomal Rna Genesmentioning

confidence: 99%

“…Concurrently, also Pneumocystis of different animal species have been more thoroughly analyzed during the past years (Aliouat- Denis et al 2008). The genomic regions of interest were primarily the large and small subunits of the mitochondrial as well as the nuclear ribosomal RNA genes (Akbar et al 2012;Board et al 2003;Chab e et al 2010;Cushion 1998;Demanche et al 2001;English et al 2001;Esgalhado et al 2013;Guillot et al 2004;Hsueh et al 2001;Hugot et al 2003;Keely et al 2004;Kitada et al 1991;Latouche et al 1994;Li et al 2008;Liu et al 1992;Mazars et al 1997;Norris et al 2003;Ortiz-Rivera et al 1995;Ortona et al 1995;Peters et al 1994a,b;Sakakibara et al 2013;Sanches et al 2011;Shah et al 1996;Sinclair et al 1991;Stringer et al 1993;Testa et al 1997;Wakefield et al 1992). As a consequence, hundreds of sequences for these genomic regions from different countries all over the world have been published and are now available in different databases.…”

mentioning

confidence: 99%

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Finding your Way Through Pneumocystis Sequences in the NCBI Gene Database

Weissenbacher‐Lang

Nedorost

Weissenböck

2014

J Eukaryotic Microbiology

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Pneumocystis sequences can be downloaded from GenBank for purposes as primer/probe design or phylogenetic studies. Due to changes in nomenclature and assignment, available sequences are presented with a variety of inhomogeneous information, which renders practical utilization difficult. The aim of this study was the descriptive evaluation of different parameters of 532 Pneumocystis sequences of mitochondrial and ribosomal origin downloaded from GenBank with regard to completeness and information content. Pneumocystis sequences were characterized by up to four different names. Official changes in nomenclature have only been partly implemented and the usage of the "forma specialis", a special feature of Pneumocystis, has only been established fragmentary in the database. Hints for a mitochondrial or ribosomal genomic origin could be found, but can easily be overlooked, which renders the download of wrong reference material possible. The specification of the host was either not available or variable regarding the used language and the localization of this information in the title or several subtitles, which limits their applicability in phylogenetic studies. Declaration of products and geographic origin was incomplete. The print version of this manuscript is completed by an online database which contains detailed information to every accession number included in the meta-analysis.

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Section: Discussionmentioning

confidence: 90%

“…suis mtLSU sequence published in the same article using the same primers (Sanches et al. ). BLAST analysis of acc.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…no. KC494265-KC494279 are assigned to the 16S rRNA in GenBank (Sanches et al 2011). Also the primer sequences are published, but they are designed for detection of mtLSU sequences.…”

Section: The Nuclear Ribosomal Rna Genesmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Finding your Way Through Pneumocystis Sequences in the NCBI Gene Database

Weissenbacher‐Lang

Nedorost

Weissenböck

2014

J Eukaryotic Microbiology

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Rethinking host range in Pneumocystis

2020

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Detection ofPneumocystisinfections by in situ hybridization in lung samples of Austrian pigs with interstitial pneumonia

et al. 2013

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Pneumocystis carinii f. sp. suis is a fungus multiplying in the respiratory tract of pigs which occasionally is associated with interstitial pneumonia. Identification of Pneumocystis in tissue samples is considered difficult and there are only scarce data on its occurrence in European pigs. This investigation presents an in situ hybridization (ISH) procedure for identification of Pneumocystis spp. in paraffin wax embedded tissue samples and its application for labeling the agent in lung samples of pigs with interstitial pneumonia. Thirty-two out of 100 lung samples from pigs on Austrian farms were identified as positive, five of them with multiple, 12 with moderate and 15 with few organisms but Grocott’s methenamine silver staining demonstrated that only 20 cases were unequivocally positive for Pneumocystis carinii. In addition to interstitial pneumonia Pneumocystis-positive pigs were more frequently affected with granulomatous pneumonia than Pneumocystis-negative pigs. Frequently concurrent infections with different viral or bacterial lung pathogens were noted but there was no positive correlation between Pneumocystis- and PCV-2-infections. With other infections, no clear-cut differences between Pneumocystis-positive and Pneumocystis-negative animals were found. This study shows that Pneumocystis infections occur frequently in Austrian pigs with interstitial pneumonia. It remains to be shown which are the factors triggering severe multiplication and whether infection with Pneumocystis alone is able to induce lung disease in pigs.

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Phylogenetic analysis of Pneumocystis from pig lungs obtained from slaughterhouses in southern and midwestern regions of Brazil

Cited by 6 publications

References 19 publications

Finding your Way Through Pneumocystis Sequences in the NCBI Gene Database

Finding your Way Through Pneumocystis Sequences in the NCBI Gene Database

Rethinking host range in Pneumocystis

Detection ofPneumocystisinfections by in situ hybridization in lung samples of Austrian pigs with interstitial pneumonia

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