Morfofisiologia do tecido intertubular e das células de Leydig de jaguatirica (Leopardus pardalis) adulta

Sarti, Priscilla; Paula, Tarcízio Antônio Rêgo de; Polli, G. O; Souza, Thyara Deco; Araújo, Gediendson Ribeiro de

doi:10.1590/s0102-09352011000500003

Cited by 7 publications

(5 citation statements)

References 6 publications

(5 reference statements)

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“…ecaudata (0.005%) was smaller to that observed in other bat species, whose average ranged from 0.015% to 0.04% [14, 35]. This index was close to that found in other mammals, such as mice and ocelots, 0.007% and 0.0036% respectively [36, 37]. Both the nuclear diameter of Leydig cells and their volumes were larger than those found for other bat species and other mammals [14, 17, 33, 38, 39].…”

Section: Discussionsupporting

confidence: 76%

Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata

Silva

Dias

et al. 2019

PLoS ONE

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Diphylla ecaudata is a hematophagous bat endemic of South America, with food preference for bird blood. Given the lack of information about the reproductive activity of this species, this study aimed to describe the testicular morphology and histomorphometry of D. ecaudata in order to understand its reproductive biology, specially spermatogenesis. The animals were collected in Lajes city, Rio Grande do Norte, Brazil. Following euthanasia, the testes were histologically processed for morphological, morphometric, ultrastructural and immunohistochemical analyses. Their average body weight was 24.64g, with a gonadosomatic index of 0.49%, tubulesomatic index of 0.47%, and a total of 32.20m of seminiferous tubules per gram of testis. The pre-meiotic, meiotic, and post-meiotic phases accounted for 56.20%, 9.30%, and 34.50% of the seminiferous epithelium cycle, respectively. The ultrastructure of spermiogenesis was similar to that described in other mammals and the perforatorium was not observed in the sperm. Androgen receptors were detected in Sertoli cell nuclei and Leydig cell cytoplasm, while aromatase enzyme was detected only in Sertoli cell nuclei. FGF2 and BCL-2 activities were detected in the cytoplasm of zygotene and pachytene primary spermatocytes, as well as round and elongated spermatids. D. ecaudata showed testicular pattern similar to other mammals and characteristics common to other bat species. This species stood out for its high efficiency of Sertoli cells, which presented high capacity to support germ cells, besides the highest sperm production rates among those already recorded. This study is the first step towards the knowledge of D. ecaudata reproduction and the first description of its spermatogenesis.

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Section: Discussionsupporting

confidence: 76%

Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata

Silva

Dias

et al. 2019

PLoS ONE

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“…molossus [27] and in S . lilium [28], however higher than in Myotis levis [35] and within the recorded for other mammals [22, 29–33]. The Leydigosomatic index was similar to that observed in other bats and higher than the observed in larger animals [21, 27, 28, 30, 32, 33].…”

Section: Discussionsupporting

confidence: 73%

“…Leydig cells were the main component in the intertubular space, which also follows the pattern previously described for other bats. In vampire bat, the percentage of Leydig cells was lower compared to frugivorous and insectivorous bats and consequently the percentage of lymphatic space was larger [ 21 , 27 , 28 ], yet within the numbers reported for other mammals [ 22 , 29 – 33 ]. The larger investment in Leydig cells found in bats might also be associated to their reproductive behavior, as high levels of testosterone would be required for the maintenance of spermatogenesis and male dominance in harem colonies [ 23 , 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

et al. 2017

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Among all bat species, Desmodus rotundus stands out as one of the most intriguing due to its exclusively haematophagous feeding habits. However, little is known about their spermatogenic cycle. This study aimed at describing the spermatogenic process of common vampire bats through testicular histomorphometric characterization of adult specimens, spermatogenic production indexes, description of stages of the seminiferous epithelium cycle and estimative of the spermatogenic process duration. Morphometrical and immunohistochemical analyzes for bromodeoxiuridine were conducted under light microscopy and ultrastructural analyzes were performed under transmission electron microscopy. Vampire bats showed higher investment in gonadal tissue (gonadosomatic index of 0.54%) and in seminiferous tubules (tubulesomatic index of 0.49%) when compared to larger mammals. They also showed a high tubular length per gram of testis (34.70 m). Approximately half of the intertubular compartment was found to be comprised by Leydig cells (51.20%), and an average of 23.77x106 of these cells was found per gram of testis. The germline cells showed 16.93% of mitotic index and 2.51% of meiotic index. The overall yield of spermatogenesis was 60% and the testicular spermatic reserve was 71.44x107 spermatozoa per gram of testis. With a total spermatogenesis duration estimated at 37.02 days, vampire bats showed a daily sperm production of 86.80x106 gametes per gram of testis. These findings demonstrate a high sperm production, which is commonly observed in species with promiscuous mating system.

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“…The LSI found in D. rotundus (0.016%) was similar to that observed in other bats and higher than that observed in larger animals, such as jaguars and ocelots (0.0036%; [47,48]), which also reinforces the need for greater investment in gamete production in order to maintain harems in these polygynic species [25,32].…”

Section: Plos Onesupporting

confidence: 80%

Seasonal evaluation of spermatogenesis of the hematophagous bat Desmodus rotundus in the Caatinga biome

et al. 2020

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This study was aimed to characterize the spermatogenic process and its seasonal variation in Desmodus rotundus, in the Caatinga biome, a water-limited ecosystem, with marked water restriction during most of the year. Collections of adult animals were performed during the dry and rainy seasons, and after euthanasia, their testes were processed histologically to perform morphological, morphometric, ultrastructural and immunohistochemical analyzes. The percentage of seminiferous epithelium, number of Leydig cells per gram of testis, and population of Sertoli cells and A-type spermatogonia presented by D. rotundus were significantly higher in the rainy season, while the percentage of lumen, mitotic index, support capacity performed by Sertoli cells, and overall yield of spermatogenesis were higher in the dry season. The ultrastructure of spermatogenesis was similar to that described in other mammals, and the immunohistochemical analysis revealed activity of the aromatase enzyme in Sertoli cells, Leydig cells, spermatocytes and spermatids, as well as the presence of androgen receptors in Sertoli cells and Leydig cells. FGF2 activity was detected in primary spermatocytes in zygotene and pachytene, as well as secondary spermatocytes and rounded and elongated spermatids, while the BCL-2 protein was expressed in primary spermatocytes in zygotene and pachytene, secondary spermatocytes, and rounded spermatids. The activity of these molecules was similar in both seasons, and associated with the morphometric findings, indicates maintenance in the integrity of the seminiferous epithelium throughout the year. The seasonal study of D. rotundus spermatogenesis indicates a continuous spermatogenesis pattern and suggests a greater production of spermatozoa in the rainy season in the Caatinga biome.

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Morfofisiologia do tecido intertubular e das células de Leydig de jaguatirica (Leopardus pardalis) adulta

Cited by 7 publications

References 6 publications

Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata

Testicular characterization and spermatogenesis of the hematophagous bat Diphylla ecaudata

The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

Seasonal evaluation of spermatogenesis of the hematophagous bat Desmodus rotundus in the Caatinga biome

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