Bone regeneration in mandible defect with autograft bone and cell suspension from bone marrow in rabbits

Abstract

The objective of this study was to investigate the bone regeneration of a "gold standard" (autograft) from iliac crest associated with cellular therapy in rabbits. A bone defect was created with 10x5x5mm in 28 rabbit mandibles. The control group animals (n=14) were repaired with autograft of iliac crest and the experimental group animals (n=14) received iliac crest autograft in association with mononuclear cells from the bone marrow of the femur. Weekly radiographs were taken of the surgery region and histological analyses was performed in seven animals in each group at 15 days and in seven animals of each group at 30 days after the surgery. A gradual increase of bone density was observed and the experimental animals presented the bone bridge in 85.7% (6/7) of the cases, while only 42.8% (3/7) of the animals in the control group presented this structure 28 days after the surgery. The histopathological parameters analyzed did not show any statistical difference between the control and experimental group in 15 and 30 days of analysis. The results suggest that the mononuclear cells from the marrow bone can better support the autograft regeneration in mandible defects in rabbits.Keywords: rabbit, mandibular reconstruction, bone regeneration, graft RESUMO Avaliou-se a regeneração óssea de auto-enxerto, considerado "padrão ouro" da crista ilíaca associado à terapia celular da medula óssea em coelhos. Foi criado um defeito ósseo de 10x5x5mm na mandíbula de

Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits

“…In autograft, the gap was almost filled with callus but complete union was lacking as compared to groups B and group C (Fig 2). Similar observations were made by the study of Gomes (2011), in radiographs after 21 days of surgery with no bone callus bridging. Remodelling was not found in initial stage of fracture repair in any of the groups by day 30, because of action of osteoblastic cells (Lieberman et al, 2002).…”

The Effect of Mineral Trioxide Aggregate Alone and in Combination With Biphasic Calcium Phosphate on Healing of Mandibular Defects in Rabbit

“…The mean values of the percentages of bone surface area formed during the healing of the bone defects in the MTA group and the MTA+BCP group after 6 weeks were calculated and demonstrated in table (1). The table reveals that the amount of the surface area of the newly formed bone is greater in MTA group than in MTA+BCP group and even the difference is significant, revealing a positive effect of the combination of MTA with BCP.…”

“…Mandibular bone defects in both human beings and animals may be caused by removal of large cystic lesion, by trauma or by infections. The techniques of mandibular reconstruction have represented a challenge to facial surgeons and orthopedists (1).…”

Evaluation of the Osteoinductive Potential of Freeze-Dried Human Amniotic Membrane (Experimental Study)

“…The repair of bone defects has always represented a huge challenge in the field of dentistry, as many injuries result in functional or esthetic impairment that remarkably reduces the quality of life of the individuals affected (1). Bone loss in the maxillofacial area may arise as a result of infections, trauma, cysts, neoplasia or congenital defects (2). These defects can range in size from small defects that can heal spontaneously to large critical-sized defects (CSD) that will neither heal spontaneously (3) nor regenerate more than 10 % of the lost bone during the patient's lifetime (4).…”