Antimicrobial susceptibility of coagulase-negative staphylococci isolated from meat-producing ewes with mastitis

Libera, Alice Maria Melville Paiva Della; Blagitz, Maiara Garcia; Souza, Fernando Nogueira de; Batista, Camila Freitas; Azedo, Milton Ricardo; Benites, N. R.; Melville, Priscila Anne; Gomes, Viviani

doi:10.1590/s0102-09352010000600030

Cited by 5 publications

(3 citation statements)

References 9 publications

(5 reference statements)

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“…In this study, the main pathogen group in all milk samples was staphylococci (77.0%), in agreement with the other studies (Ergün et al 2009;Della Libera et al 2010;Virdis et al 2010;Kunz et al 2011).…”

Section: Discussionsupporting

confidence: 93%

Panton–Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants

Ünal

Aşkar

Macun

et al. 2011

Trop Anim Health Prod

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The aims of this study were to determine the existence of pvl gene, some toxin genes, and mecA gene in Staphylococcus aureus strains isolated from sheep milk and to examine antimicrobial resistance profiles in staphylococci from sheep and goats' milk. The milk samples were collected from 13 different small ruminant farms in Kirikkale province from February to August 2009. A total of 1,604 half-udder milk samples from 857 ewes and 66 half-udder milk samples from 33 goats were collected. Staphylococcus spp. were isolated and identified from the samples. Toxin genes and mecA gene among S. aureus strains were determined by PCR. Antimicrobial susceptibility of staphylococci was examined by the disk diffusion method on Mueller-Hinton agar, and interpreted according to the Clinical Laboratory Standards Institute (CLSI) guidelines. The prevalence of subclinical intramammary infection in both ewes and goats was 5.2%. The most prevalent subclinical mastitis agents were coagulase-negative staphylococci and S. aureus with prevalences 2.8% (n:46) and 1.3% (n = 21), respectively. The prevalence of resistances in isolated Staphylococcus spp. to penicilin G, tetracycline, erythromycin, gentamicin, and enrofloxacin were found as 26.9% (18), 7.5% (5), 6.0% (4), 3.0% (2), and 1.5% (1), respectively. Only 3 of the 21 S. aureus ewe isolates (13.4%) were shown to harbor enterotoxin genes being either seh, sej or sec. However, fourteen (66.6%) of the 21 S. aureus isolates had pvl gene while none of the isolates harbored mecA gene. In conclusion, Staphylococci were shown to be the most prevalent bacteria isolated from subclinical mastitis of ewes and goats and these isolates were susceptible to most of the antibiotics. In addition, S. aureus strains isolated from ewes were harboring few staphylococcal enterotoxin genes. However, Panton-Valentine leukocidin produced by S. aureus could be an important virulence factor and contribute to subclinical mastitis pathogenicity.

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Section: Discussionsupporting

confidence: 93%

Panton–Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants

Ünal

Aşkar

Macun

et al. 2011

Trop Anim Health Prod

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“…The high effectiveness of the tested antibiotics against the agent may be related to the elevated percentage of isolates classified as coagulase-negative, considering that some researchers have reported that strains of this agent isolated from bovines showed resistance less frequently than coagulase-positive species. On the other hand, these agents are known to be emergent mammalian pathogens, and the frequent use of antibiotics against mastitis may cause the emergence of multidrug-resistant strains of coagulase-negative Staphylococcus (DELLA LIBERA et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Muscoid Diptera as potential vectors of bacterial agents on dairy farms in the northern region of Paraná, Brazil

et al. 2014

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The aim of this study was to determine the isolation frequency and microbial sensitivity profiles of Escherichia coli, Salmonella spp., and Staphylococcus spp. isolated from synanthropic flies captured in entomological traps placed near the milking parlors of 30 dairy farms located in Northern Paraná, Brazil. In total, 192 flies were captured, and the isolation frequencies of muscids (21/30 = 70%) and califorids (27/30 = 90%) were significantly higher than that of sarcophagids (7/30 = 23.3%). Bacteriological tests on the internal contents and external surfaces of the flies showed that E. coli was present only in muscids (14.3%) and caliphorids (33.3%). Salmonella spp. was isolated from 9.5% of the muscids, 7.4% of the caliphorids, and 14.29% of the sarcophagids. Staphylococcus spp. was isolated from 28.5% of the muscids and 29.6% of the caliphorids. E. coli isolation was more frequent in flies captured on farms at which domestic chickens were housed near the milking parlors (p = 0.031) and which did not use sugarcane for animal feed (p = 0.042). Two out of 27 (7.4%) Staphylococcus spp. strains presented a phenotype of coagulase enzyme production. Ceftriaxone, ciprofloxacin, enrofloxacin, and gentamicin were the most effective antibiotics against E. coli and Salmonella spp. Tetracycline was the least effective antibiotic against the isolates. The Staphylococcus spp. strains isolated did not show the phenotype of oxacillin resistance. We conclude that regional flies are potential mechanical vectors of microbial agents able to cause enteritis in goats, mastitis in cows, and contamination of milk products for human consumption. Key words: Synanthropic flies, dairy cattle, and infectious agents ResumoMoscas sinantrópicas são vetores mecânicos de enfermidades infecciosas para humanos e animais. O presente estudo pretendeu avaliar a presença de Escherichia coli, Salmonella e Staphylococcus spp na população de moscas das famílias Calliphoridae, Muscidae e Sarcophagidae capturadas em 30 fazendas de leite na região Norte do Paraná. As capturas foram realizadas com armadilhas entomológicas contendo iscas a base de fígado bovino, penduradas em árvores próximas as salas de ordenhas das fazendas. Lavados da superfície externa e fluídos extraídos do abdome esmagado das moscas foram semeados em agar sangue ovino a 5%, agar Mac Conkey e agar Baird-Parker e submetidos a enriquecimento seletivo para Salmonella em caldo Rappaport-Vassiliadis e tetrationato Muller-Kauffmann, seguido de (33,3%). Salmonella foi isolada de 9,5% dos muscídeos, 7,4% dos califorídeos e de 14,29% dos sarcofagídeos capturados. Staphylococcus spp foi isolado de 28,5% dos muscídeos e 29,6% dos califorídeos. Os isolamentos de E. coli foram mais comuns e moscas capturadas em fazendas que mantinham criações de aves próximas ao local (p = 0,031) de ordenha e que não utilizavam cana-de-açúcar para alimentação dos animais (p=0,042). Conclui-se que as moscas presentes em fazendas de leite da região apresentam grande importância sanitária, visto que os agent...

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“…A identificação do grupo SCN foi realizada pela avaliação macroscópica e microscópica das colônias, provas da catalase e coagulase (DELLA LIBERA et al, 2010); para Staphylococcus aureus também foram utilizados os sistemas de identificação RapID ® (Remel -Oxóid); para Corynebacterium spp. foi realizada pela prova da catalase e identificação macro e microscópica das colônias.…”

Section: Methodsunclassified

Etiologia e fatores de risco para a infecção mamária de cabras leiteiras em seis propriedades do Estado de São Paulo

et al. 2014

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ResumoO objetivo do trabalho foi identificar os agentes etiológicos e fatores de risco para a mastite carpina. Para tanto, foram colhidas duas alíquotas de 257 amostras de leite, nos meses compreendidos entre Abril a Setembro de 2012, provenientes de 136 cabras da raça Saanen, oriundas de seis criações intensivas, com sistema de ordenha manual e mecânica, localizadas no estado de São Paulo. A primeira alíquota das amostras de leite foi obtida em frascos contendo o conservante bronopol para a contagem de célula somática (CCS) eletrônica, e a segunda em frascos estéreis para o exame bacteriológico. Para avaliação dos fatores de risco, foram aplicados questionários epidemiológicos preenchidos pela equipe de pesquisa mediante observações visuais e inquérito com tratadores e proprietários. Foram realizadas análises univariada e multivariada, utilizando-se a regressão logística múltipla. Observou-se prevalência de 28,79% de infecção mamária com predomínio de Staphylococcus coagulase negativa (SCN). A mediana da CCS obtida foi de 4,7x10 5 células por mL (céls mL -1 ) de leite. Na análise univariada foi possível observar risco para a infecção mamária nos locais onde a caprinocultura não era a atividade principal da propriedade (p=0,03); a prova do fundo escuro não era realizada (p=0,07); não era utilizado o cloro na limpeza dos tetos (p=0,29); realizava-se limpeza inadequada da sala de ordenha (p=0,06); observou-se higiene inadequada no processo de ordenha (p=0,03) e a higiene do ordenhador era inadequada (p=0,03). A higiene dos tetos, da sala, do processo de ordenha e ordenhador foram os maiores riscos associados à infecção mamária por SCN. Desta forma, apesar das indicações de medidas higiênicas nos programas de qualidade do leite e controle de mastite, a aplicabilidade e conscientização do pessoal envolvido com a cadeia produtiva ainda não estão sensibilizadas. Assim, o treinamento de pessoal seria a principal medida recomendada para o controle da mastite em cabras leiteiras. Palavras-chave: Contagem de célula somática, exame bacteriológico, mastite, saanen, Staphylococcus coagulase-negativa

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Antimicrobial susceptibility of coagulase-negative staphylococci isolated from meat-producing ewes with mastitis

Cited by 5 publications

References 9 publications

Panton–Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants

Panton–Valentine leukocidin and some exotoxins of Staphylococcus aureus and antimicrobial susceptibility profiles of staphylococci isolated from milks of small ruminants

Muscoid Diptera as potential vectors of bacterial agents on dairy farms in the northern region of Paraná, Brazil

Etiologia e fatores de risco para a infecção mamária de cabras leiteiras em seis propriedades do Estado de São Paulo

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