Mast cells and angiogenesis in canine mammary tumor

Lavalle, Gleidice Eunice; Bertagnolli, Angélica Cavalheiro; Tavares, Wanessa Luciene Fonseca; Ferreira, Mônica Alves Neves Diniz; Cassali, G. D.

doi:10.1590/s0102-09352010000600008

Cited by 9 publications

(6 citation statements)

References 12 publications

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“…The blocked sections were incubated overnight at 48°C anti-mouse primary antibodies for TP PD-ECGF AB1 (clone PGF 44.C) (Neo Markes, Freemount, California, USA, diluted 1/100) (15) and monoclonal mouse antibody anti-CD31 Clone JC70A (Dako, Glostrup, Denmark) diluted 1/50, for 40 min then thoroughly washed in 0.05 M buffered with Tris saline (pH = 7.6) and incubated with streptavidin-peroxidase (Dako, 1: 100) for 40 min. 3, 3- diaminobenzidine (DAB) (Dako Glostrup, Denmark) was used as the chromogen; to counteract the core of Gill'S hematoxylin (Polysciences, Warrington, PA, USA) after sections have been dehydrated and assembled (34, 35). We do also have evidence of negative controls not immunostained and positive control of not tumor cells from canine expressing TP that show sporadic area of immunostaining.…”

Section: Methodsmentioning

confidence: 99%

Thymidine Phosphorylase Expression and Microvascular Density Correlation Analysis in Canine Mammary Tumor: Possible Prognostic Factor in Breast Cancer

et al. 2019

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Purpose: The thymidine phosphorylase (TP) is a key enzyme involved in the metabolism of pyrimidines. Inhibition or downregulation of this enzyme causes accumulation of metabolites with consequences in DNA replication. TP regulates angiogenesis and chemotactic activity of endothelial cells. Different studies showed the presence of TP upregulation in human cancer but the correlation between TP expression and the microvascular density (MVD) in canine mammary tumors is unknown. The aim of this study was to investigate a possible correlation between the MVD and TP expression in tumor cells of canine mammary tumors of different degree of severity (G1–G3) by immunohistochemical analysis.Methods: Sixty-eight samples of spontaneous mammary neoplasia of 5–12 cm in diameter were collected from purebred and mixed-breed dogs (mean aged = 9.5 ± 7), not subject to chemotherapy treatments in veterinary clinics. Histopathological analysis and immunostaining were performed.Results: Carcinoma simple samples have been classified as 72.06% of tubule-papillary, 20.59% cysto-papillary, and 7.35% tubular carcinomas. Immunostainings revealed a marked cytoplasmic expression of TP in 30.88% of samples, mild in 32.35%, weaker in 22.07%, and negative in 14.70%. The correlation analysis and two-way ANOVA showed a linear correlation between MVD and TP with a coefficient of correlation (r) > 0.5 (p < 0.05) in G2 and G3. No correlation between variables was found in G1.Conclusions: These findings suggest that cytoplasmic TP overexpression is correlated with microvascular density in canine mammary tumors, in severe grade, and it can be a potential prognostic factor in breast cancer.

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Section: Methodsmentioning

confidence: 99%

Thymidine Phosphorylase Expression and Microvascular Density Correlation Analysis in Canine Mammary Tumor: Possible Prognostic Factor in Breast Cancer

et al. 2019

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“…Moreover, IL-28 secreted by the MDSCs stimulates the STAT3 pathway which increases VEGF-C expression and therefore induces angiogenesis [55]. A positive correlation between microvessel density and mast cells in malignant canine mammary cancer was found, which, in turn, suggests that these immune cells play an important role in canine mammary cancer angiogenesis, similar to human breast cancer [53]. TAMs in canine mammary cancer have already been associated, by our group, with skin ulceration, histological type, nuclear grade, tubular differentiation, and decrease in overall survival [56].…”

Section: Inflammatory Cells and Sustained Angiogenesismentioning

confidence: 99%

A Comparative Approach of Tumor-Associated Inflammation in Mammary Cancer between Humans and Dogs

Carvalho

Silva-Carvalho

Pires

et al. 2016

BioMed Research International

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Infiltrating cells of the immune system are widely accepted to be generic constituents of tumor microenvironment. It has been well established that the development of mammary cancer, both in humans and in dogs, is associated with alterations in numbers and functions of immune cells at the sites of tumor progression. These tumor infiltrating immune cells seem to exhibit exclusive phenotypic and functional characteristics and mammary cancer cells can take advantage of signaling molecules released by them. Cancer related inflammation has an important role in mammary carcinogenesis, contributing to the acquisition of core hallmark capabilities that allow cancer cells to survive, proliferate, and disseminate. Indeed, recent studies in human breast cancer and in canine mammary tumors have identified a growing list of signaling molecules released by inflammatory cells that serve as effectors of their tumor-promoting actions. These include the COX-2, the tumor EGF, the angiogenic VEGF, other proangiogenic factors, and a large variety of chemokines and cytokines that amplify the inflammatory state. This review describes the intertwined signaling pathways shared by T-lymphocytic/macrophage infiltrates and important tissue biomarkers in both human and dog mammary carcinogenesis.

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“…The presence and activity of mast cells in the tumor microenvironment remain controversial. Some studies reported an association between high number of profile cells of mast cells in the tumor microenvironment, greater release of angiogenic factors and high number of profile cells of microvasculature in human and canine mammary carcinomas (Cimpean et al, 2017;Lavalle et al, 2010). On the other hand, Amini et al (2007), Dabiri et al (2004) and Rajput et al (2008) observed, in human mammary carcinomas, that a high number of profile cells of mast cells was related to low grade tumors and estrogen receptor positivity, to patients without lymph node involvement and to patients with better prognosis, respectively.…”

Section: Introductionmentioning

confidence: 99%

Relationship between the inflammatory tumor microenvironment and different histologic types of canine mammary tumors

Souza

Campos

Gonçalves

et al. 2018

Research in Veterinary Science

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Mammary neoplasms are the tumors with higher incidence in female dogs. Among the factors that contribute for the development of this and other neoplasms, the inflammatory tumor microenvironment plays a crucial role. Several studies reported important roles for lymphocytes, macrophages, plasma cells, neutrophils, eosinophils and mast cells in this context. In the present study, our aim was to evaluate the number of profile cells of inflammatory cells and area of tumor fibrosis and the relation of these features with canine mammary tumors of different histologic and clinical presentation (benign mixed tumor, carcinoma in mixed tumor, solid carcinoma and tubular carcinoma) Counting and staining of inflammatory cells and tumor fibrosis were performed through histochemistry, while counting and staining of CD4, TCD8 and FOXP3 lymphocytes were performed through immunohistochemistry. Statistical analysis of the association between densities of inflammatory cells, tumor fibrosis and histologic types revealed significant difference for plasma cells (p = .035), neutrophils (p = .0113), macrophages (p = .0047), and tumor fibrosis (p = .05). The found data suggest associations between high number of neutrophils and aggressive mammary tumors, between high densities of plasma cells, macrophages and CD8 cells and between low number of profile cells of CD4 cells and less aggressive tumors. Larger areas of tumor fibrosis showed relation to more aggressive canine mammary tumors.

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Mast cells and angiogenesis in canine mammary tumor

Cited by 9 publications

References 12 publications

Thymidine Phosphorylase Expression and Microvascular Density Correlation Analysis in Canine Mammary Tumor: Possible Prognostic Factor in Breast Cancer

Thymidine Phosphorylase Expression and Microvascular Density Correlation Analysis in Canine Mammary Tumor: Possible Prognostic Factor in Breast Cancer

A Comparative Approach of Tumor-Associated Inflammation in Mammary Cancer between Humans and Dogs

Relationship between the inflammatory tumor microenvironment and different histologic types of canine mammary tumors

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