Detecção dos genes da toxina citoletal distensiva em estirpes de Campylobacter jejuni isoladas de carcaças de frangos

Carvalho, Aline Feola de; Silva, D.M.; Azevêdo, Sérgio Santos de; Piatti, Rosa Maria; Genovez, Margareth Élide; Scarcelli, E.

doi:10.1590/s0102-09352010000500006

Cited by 13 publications

(5 citation statements)

References 26 publications

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“…The isolation and biochemical identification of C. jejuni and C. coli was performed according to the Bacteriological Analytical Manual (Hunt et al , 2001; Carvalho et al , 2010). …”

Section: Methodsmentioning

confidence: 99%

Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil

Carvalho¹,

Silva²,

Azevêdo³

et al. 2013

Braz. J. Microbiol.

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Campylobacteriosis is a worldwide distributed zoonosis. One of the main virulence factors related to Campylobacter spp. in animals and humans is the cytolethal distending toxin (CDT), encoded by three adjacent genes (cdtA, cdtB, cdtC). The occurrence of Campylobacter spp. in samples of vegetables has not been reported in Brazil yet, and has seldom been described in the international literature. The detection of CDT in these strains has not been reported, either. The objectives of the present study were to determine the occurrence of Campylobacter spp. strains carrying virulence factors in samples of poultry and vegetables (lettuce and spinach) from different points of sale, thus verifying if vegetables are as an important vehicle for potentially virulent Campylobacter spp. strains as poultry. Twenty four strains were identified as Campylobacter jejuni by phenotypic and genotypic methods: 22 from broiler carcasses and two from lettuce samples. Three strains were identified as Campylobacter coli: two from broiler carcasses and one from lettuce. The presence of the cdt genes were detected in 20/24 (83.3%) C. jejuni strains, and 3/3 (100%) C. coli strains. The isolation of Campylobacter spp. strains with the cdt gene cluster in lettuce samples points to a new possible source of contamination, which could have an impact in the vegetable production chain and risk to public health. Results show that potentially virulent C. jejuni and C. coli strains remain viable in samples of broiler carcasses and vegetables at the points of sale.

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“…The isolation and biochemical identification of C. jejuni and C. coli was performed according to the Bacteriological Analytical Manual (Hunt et al , 2001; Carvalho et al , 2010). …”

Section: Methodsmentioning

confidence: 99%

Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil

Carvalho¹,

Silva²,

Azevêdo³

et al. 2013

Braz. J. Microbiol.

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“…The tests performed at 400 chicken carcasses indicated major presence of the bacterium in the carcass sold in the central region than in peripheral region (Modolo et al, 2005). However, Carvalho et al (2010) found lower percentage of isolation of these bacteria, 14.2%. Silva et al (2014) determined occurrences in 61% of samples of chicken droppings, 20% in chicken products for consumption, and 3% in human stool, all linked to this microorganism.…”

Section: Discussionmentioning

confidence: 76%

“…Silva et al (2014) determined occurrences in 61% of samples of chicken droppings, 20% in chicken products for consumption, and 3% in human stool, all linked to this microorganism. Carvalho et al (2010) pointed out the presence of gene complexes of the extensional citoletal toxin (CDT) in 36.4% of the samples. However, Silva et al (2014) determined 93.5% of such grouped genes for CDT.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have primarily described the presence of Campylobacter spp., Salmonella spp. and E. coli in poultry products (Zhao et al, 2001;Cardinale et al, 2003;Modolo et al, 2005;Ha and Pham 2006;Rall et al, 2009;Carvalho et al, 2010;Silva et al, 2014). Based on these grounds, this research aims to identify the presence of Campylobacter, Salmonella and E. coli in broiler carcasses in Botucatu, SP, Brazil, by microbiological and molecular methods.…”

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confidence: 99%

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Occurrence of Campylobacter spp., Salmonella spp. and Escherichia coli in Chicken Carcasses

Machado

Okamoto

Modolo

et al. 2017

IJAVST

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The transmission of Foodborne Diseases (FBD) by bacteria constitute a public health problem in the world. This study aimed to identify the presence of Campylobacter spp., Salmonella spp. and E. coli in broiler chickens' carcasses of Botucatu, SP, Brazil, by means of microbiological and molecular methods. Sixty samples of chilled chickens' carcasses of different brands were randomly collected from supermarkets and meat houses located in both peripheral and central area of the city, from January 2015 to January 2016. Later, Campylobacter, Salmonella and E. coli was isolated from poultry carcasses by conventional microbiological methods, and confirmed by biochemical and PCR tests. The prevalence was 38.3% of Campylobacter, Salmonella, 13.3%, and E. coli, 60%.

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“…We examined mares in the Wielkopolska region for occurrence of Campylobacter bacteria and found that 37% of the isolates (n = 6/16) had cdtB gene [5] and did not find the cdtA or cdtC gene among test isolates. These genes are commonly found in poultry, cats, dogs and piglets [6]. However, there have been very few studies reporting their presence in Campylobacter isolates from horses.…”

Section: Resultsmentioning

confidence: 99%

An Assessment of the Occurrence of Selected Virulence and Antibiotic Resistance Genes in Bacteria of the Genus Campylobacter Collected from Horses

Selwet

2020

Open Veterinary Science

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AbstractThe aim of the study was to determine the frequency of Campylobacter spp. bacteria occurrence in female Hutsul horses, and the presence of selected virulence genes and resistance to antibiotics among the isolates obtained in the experiment. One hundred (n = 100) healthy mares were tested. Campylobacter spp. bacteria were found in 9 (n = 9) animals, 89% (n = 8) of which were described as C. jejuni. There were no C. coli or C. lari found. Analyses of C. jejuni isolates revealed the presence of selected virulence genes in the following order: cadF (n = 5), cdtA (n = 1) and cdtB (n = 3). The following antibiotics were used to test the antibiotic resistance of the bacteria: ampicillin (AMP), ciprofloxacin (CIP), erythromycin (E), gentamicin (GE), meropenem (MEM) and tetracycline (TE). C. jejuni were completely sensitive to ciprofloxacin, gentamicin, meropenem, and tetracycline.

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Detecção dos genes da toxina citoletal distensiva em estirpes de Campylobacter jejuni isoladas de carcaças de frangos

Cited by 13 publications

References 26 publications

Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil

Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil

Occurrence of Campylobacter spp., Salmonella spp. and Escherichia coli in Chicken Carcasses

An Assessment of the Occurrence of Selected Virulence and Antibiotic Resistance Genes in Bacteria of the Genus Campylobacter Collected from Horses

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