Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks

Radulović,; Milutinović, Milica; Tomanović, Snežana; Mulenga, Albert

doi:10.1590/s0102-09352010000400015

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…Slide microscopy was performed after standard Giemsa staining of blood films, DNA extraction and PCR targeting 16S rRNA [ 10 ], flaB [ 11 ] and glp Q [ 12 ] followed by sequencing was done from 1 ml ethylenediaminetetraacetic acid (EDTA)-blood as described previously [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

Screening for infectious diseases among newly arrived asylum seekers, Bavaria, Germany, 2015

et al. 2018

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Background and aimAs a consequence of socioeconomic and political crises in many parts of the world, many European Union/European Economic Area (EU/EEA) countries have faced an increasing number of migrants. In the German federal state of Bavaria, a mandatory health screening approach is implemented, where individuals applying for asylum have to undergo a medical examination that includes serological testing for HIV and hepatitis B, screening for tuberculosis, and until September 2015, stool examination for Salmonella spp. and Shigella spp.. Methods: Data from mandatory screening of all first-time asylum seekers in Bavaria in 2015 was extracted from the mandatory notification and laboratory information system and evaluated. Results: The HIV positivity and hepatitis B surface antigen (HBsAg) positivity rate of tested samples from asylum seekers were 0.3% and 3.3%, respectively, while detection rate of active tuberculosis was between 0.22% and 0.38%. The rates for HIV, hepatitis B, and tuberculosis among asylum seekers were similar to the corresponding prevalence rates in most of their respective countries of birth. Only 47 Salmonella spp. (0.1%) were isolated from stool samples: 45 enteric and two typhoid serovars. Beyond mandatory screening, louse-borne relapsing fever was found in 40 individuals. Conclusions: These results show that mandatory screening during 2015 in Bavaria yielded overall low positivity rates for all tested infectious diseases in asylum seekers. A focus of mandatory screening on specific diseases in asylum seekers originating from countries with higher prevalence of those diseases could facilitate early diagnosis and provision of treatment to affected individuals while saving resources.

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Section: Methodsmentioning

confidence: 99%

Screening for infectious diseases among newly arrived asylum seekers, Bavaria, Germany, 2015

et al. 2018

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“…A novel Borrelia genospecies was detected and characterised by PCR amplification of the 5S-23S rRNA intergenic spacer (Rijpkema, Molkenboer, Schouls, Jongejan, & Schellekens, 1995), and subsequent sequencing of PCR amplicons of the 16S rRNA locus (Radulović, Milutinovic, Tomanovic, & Mulenga, 2010) and of the clpX gene (Margos et al, 2008).…”

Section: Molecular Analysismentioning

confidence: 99%

Host dispersal shapes the population structure of a tick‐borne bacterial pathogen

Norte

Margos²,

Becker

et al. 2020

Molecular Ecology

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Birds are hosts for several zoonotic pathogens. Because of their high mobility, especially of longdistance migrants, birds can disperse these pathogens, affecting their distribution and phylogeography. We focused on Borrelia burgdorferi sensu lato, which includes the causative agents of Lyme borreliosis, as an example for tick‐borne pathogens, to address the role of birds as propagation hosts of zoonotic agents at a large geographical scale. We collected ticks from passerine birds in 11 European countries. B. burgdorferi s.l. prevalence in Ixodes spp. was 37% and increased with latitude. The fieldfare Turdus pilaris and the blackbird T. merula carried ticks with the highest Borrelia prevalence (92 and 58%, respectively), whereas robin Erithacus rubecula ticks were the least infected (3.8%). Borrelia garinii was the most prevalent genospecies (61%), followed by B. valaisiana (24%), B. afzelii (9%), B. turdi (5%) and B. lusitaniae (0.5%). A novel Borrelia genospecies “Candidatus Borrelia aligera” was also detected. Multilocus sequence typing (MLST) analysis of B. garinii isolates together with the global collection of B. garinii genotypes obtained from the Borrelia MLST public database revealed that: (a) there was little overlap among genotypes from different continents, (b) there was no geographical structuring within Europe, and (c) there was no evident association pattern detectable among B. garinii genotypes from ticks feeding on birds, questing ticks or human isolates. These findings strengthen the hypothesis that the population structure and evolutionary biology of tick‐borne pathogens are shaped by their host associations and the movement patterns of these hosts.

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“…In all cases, a preliminary diagnosis was established by clinicians based on clinical symptoms and visible spirochetes in Giemsa stained blood films The German National Reference Centre (NRC) for Borrelia in Oberschleißheim was contacted directly by clinicians for confirmation. Microbiological confirmation of LBRF from EDTA blood included: Dark field microscopy (Leitz Dialux 20 microscope, Leitz, Germany, objective 40, occular 10) and PCR targeting 16S rRNA, flab, and glpQ followed by sequencing [1][2][3].…”

Section: Case Investigationmentioning

confidence: 99%

Louse-borne relapsing fever (Borrelia recurrentis) diagnosed in 15 refugees from northeast Africa: epidemiology and preventive control measures, Bavaria, Germany, July to October 2015

Hoch¹,

Wieser

Löscher³

et al. 2015

Eurosurveillance

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We report 15 imported louse-borne relapsing fever (LBRF) cases in refugees in Bavaria, Germany. One patient died. Epidemiological findings confirmed that all were young males from the Horn of Africa (12 from Somalia), who had similar migration routes converging in Sudan continuing through Libya and Italy. The majority likely acquired their infection during migration. Healthcare workers should be aware of LBRF in refugees passing through north Africa to ensure correct treatment and preventive measures.

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Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks

Cited by 9 publications

References 19 publications

Screening for infectious diseases among newly arrived asylum seekers, Bavaria, Germany, 2015

Screening for infectious diseases among newly arrived asylum seekers, Bavaria, Germany, 2015

Host dispersal shapes the population structure of a tick‐borne bacterial pathogen

Louse-borne relapsing fever (Borrelia recurrentis) diagnosed in 15 refugees from northeast Africa: epidemiology and preventive control measures, Bavaria, Germany, July to October 2015

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