Microbiota bacteriana em segmentos de mosca do estábulo Stomoxys calcitrans no Brasil: primeiro relato de espécies

Castro, Bruno Gomes de; Souza, Miliane Moreira Soares de; Bittencourt, Avelino José

doi:10.1590/s0102-09352008000400039

Cited by 1 publication

(1 citation statement)

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“…They reported that addition of Acinetobacter sp., Empedobacter breve, Flavobacterium odoratum or Escherichia coli favored larval development. In another study, microbiota with pathogenic potential for humans and animals and bacteria used for biological control of arthropods were isolated from adult flies; 33 bacterial species from the families Bacillaceae, Enterobacteriaceae and Micrococcaceae were identified (CASTRO et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of Stenotrophomonas maltophilia in Stomoxys calcitranslarvae

Moraes

Videira

Bittencourt

et al. 2014

Rev. Bras. Parasitol. Vet.

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The microbiota present in Stomoxys calcitrans larvae may assist their survival in contaminated environments through production of inhibitory substances. Bacteriological identification methods, the polymerase chain reaction (PCR) and scanning electron microscopy (SEM) were used to detect a bacterium naturally present in mucus and macerated S. calcitrans larvae. The antifungal activity was determined based on the results from disk diffusion tests on an artificial solid medium. The bacterium was identified as Stenotrophomonas maltophilia and presented antifungal activity against Beauveria bassiana sensu lato isolates CG 138, CG 228 and ESALQ 986. This result suggests that the larval microbiota is a factor that can compromise the use of B. bassiana s.l. fungus for biological control of S. calcitrans larvae.Keywords: Stenotrophomonas maltophilia, Stomoxys calcitrans, antifungal activity. ResumoA microbiota presente em larvas de Stomoxys calcitrans pode auxiliar na sua sobrevivência em ambientes contaminados, devido à produção de substâncias inibidoras. Métodos bacteriológicos de identificação, reação em cadeia da polimerase (PCR) e microscopia eletrônica de varredura (MEV) foram utilizados para detectar uma bactéria naturalmente presente no muco e macerado de larvas de S. calcitrans. A atividade antifúngica foi baseada nos resultados obtidos no teste de difusão em meio sólido artificial. A bactéria foi identificada como Stenotrophomonas maltophilia e apresentou atividade antifúngica contra os isolados CG 138, CG 228 e ESALQ 986 de Beauveria bassiana sensu lato. Estes resultados sugerem que a microbiota larval é um fator que pode comprometer o uso de B. bassiana s.l. no controle biológico de larvas de S. calcitrans.

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Section: Introductionmentioning

confidence: 99%