Isolation and characterizaton of Edwardsiella tarda from pacu Myleus micans

Lima, Luciene C.; Fernandes, Antônio Alberto Ribeiro; Costa, A.; Velasco, F.O.; Leite, Rômulo Cerqueira; Hackett, J.L.

doi:10.1590/s0102-09352008000100040

Cited by 20 publications

(15 citation statements)

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“…; Lima et al . ). It causes systemic infections in fish by entering skin, gill and intestine, where it invades several kinds of cells such as epithelial cells and macrophages and replicates intracellularly (Ling et al .…”

Section: Introductionmentioning

confidence: 97%

Invasin of Edwardsiella tarda is essential for its haemolytic activity, biofilm formation and virulence towards fish

et al. 2013

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Aims: The aim of this study was to investigate the role of invasin in a bacterial fish pathogen Edwardsiella tarda. Methods and Results: In this study, an in-frame deletion mutant of invasin (Dinv) in Edw. tarda H1 was constructed through double crossover allelic exchange to explore the function of invasin in virulence to fish. Meanwhile, an invasin overexpression strain (inv + ) was obtained by electrotransformation of a low-copy plasmid pACYC184 carrying the intact invasin into the Dinv mutant. Several virulence-associated characters of the mutants and wild-type strain were tested. Compared with the wild-type H1, haemolytic activity and biofilm formation were decreased in Dinv, while increased significantly in inv + . In addition, the invasin overexpressing strain inv + exhibited increased internalization into Epithelioma Papulosum Cyprini (EPC) cells. Moreover, in zebrafish model, Dinv showed decreased virulence compared with H1, while inv + restored the virulence of wild type completely. Conclusions:The results demonstrated that invasin of Edw. tarda plays essential roles in haemolytic activity, biofilm formation, adherence, internalization and pathogenicity of this bacterium. Significance and Impact of the Study: This study revealed the role of invasin in Edw. tarda infection and provided useful information for further unveiling the pathogenesis of Edw. tarda.

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Section: Introductionmentioning

confidence: 97%

Invasin of Edwardsiella tarda is essential for its haemolytic activity, biofilm formation and virulence towards fish

et al. 2013

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“…dengan karakter bakteri berdasarkan penelitian yang telah dilakukan sebelumnya dan dilakukan pemeriksaan kesesuaian, diperoleh 15 parameter yang sesuai (Lima et al, 2008;Park et al 2012) Zona hambat ekstrak kunyit terhadap bakteri Diameter zona hambat dari kunyit dengan metode ekstraksi berbeda menunjukkan hasil terbaik pada metode ekstraksi B (dekoksi) dengan diameter rataan 7,42 mm, sedangkan diameter terkecil ditunjukkan pada metode ekstraksi A dengan tidak munculnya zona hambat. Kontrol positif menggunakan alkohol 70% menghasilkan zona hambat 6,50 mm, sedangkan pada kontrol negatif tidak terlihat adanya zona hambat.…”

Section: Pemberian Pakanunclassified

“…Sama halnya dengan Lima et al (2008), bakteri E. tarda menunjukkan gejala klinis pada infeksi ikan seperti hemoragi atau pendarahan pada tubuh, insang, perut, insang, dan ekor, juga kemunculan ulcer akibat infeksi. Bakteri E. tarda juga menimbulkan gejala klinis pada organ dalam ikan ditandai dengan abses atau pembengkakan pada usus dan adanya gas pada bagian organ dalam, juga hati dan ginjal yang pucat (Muhanty & Sahoo, 2007), hal tersebut…”

Section: Pembahasanunclassified

The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.

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Sukenda

et al. 2015

Jurnal Akuakultur Indonesia

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ABSTRACT Target production of catfish in aquaculture can be reached by suppressing the pathogenic bacterial infection. Previous works had shown that turmeric Curcuma longa has antibacterial activity. The objectives of this research were to determine the best method of extraction and to evaluate the efficacy of turmeric extract as feed additive to control Edwardsiella tarda disease in catfish culture. Briefly, the objective was achieved through in vitro assay based on inhibition ability of extraction method against E. tarda, while the following objective was obtained through in vivo assay based on their survival during challenge test either as preventive or curative measurement. A complete randomized design with three replications was used for each assay. Furthermore, challenge test was done by mean intraperitoneal injection at concentration 106 cfu/mL of E. tarda (LD50). The results showed that 15 minutes decoction method allowed the best inhibition with diameter 7.42 mm of clear zone and then curative measurement using turmeric extract could be the best application against E. tarda since it gave 86.67% of survival. Clinical signs such as swelling, hemoraghic, body ulcer, gastroentritis and gaseous captivity were observed on challenged fish. However, there were no significant different among treatments for specific growth, body weight, and absolute length parameters. Keywords: Edwardsiella tarda, extraction, turmeric, catfish, inhibition zone ABSTRAK Peningkatan produksi ikan lele dapat dicapai melalui pencegahan infeksi penyakit bakterial. Hasil beberapa penelitian membuktikan bahwa kunyit Curcuma longa terbukti memiliki zat aktif yang bersifat antibakteri. Penelitian ini bertujuan untuk mencari metode ekstraksi terbaik dan mengevaluasi efektivitas penambahan ekstrak kunyit pada pakan untuk pengendalian patogen Edwardsiella tarda pada ikan lele. Metoda ekstraksi kunyit diuji secara in vitro dengan metoda zona hambat, sedangkan efikasi diuji secara in vivo melalui perlakuan pencegahan dan pengobatan. Penelitian didesain dalam rancangan acak lengkap dengan tiga ulangan. Efikasi ekstrak kunyit diketahui dari nilai sintasan ikan lele hasil uji tantang E. Tarda melalui injeksi peritoneal dengan dosis 106 cfu/ mL (LD50). Hasil uji in vitro menunjukkan bahwa metode dekoksi selama 15 menit memberikan zona hambat terbaik yaitu sebesar 7,42 mm. Hasil uji in vivo menunjukkan bahwa ekstrak kunyit sebagai tindak pengobatan memberikan nilai sintasan terbaik, yaitu sebesar 86,67%. Ikan lele yang diuji tantang menunjukkan gejala klinis berupa pembengkakan, luka, gastroentritis, dan gas pada perut. Tidak terdapat perbedaan nyata di antara perlakuan untuk parameter laju pertumbuhan harian, bobot harian, dan pertumbuhan panjang mutlak. Kata kunci: Edwardsiella tarda, ekstraksi, kunyit, ikan lele, zona hambat

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“…Esses sinais são frequentemente relatados em casos de septicemia em peixes, provocadas por outros agentes bacterianos (Lima et al, 2008).…”

Section: Palavras-chaveunclassified

“…Esses achados estão em consonância com as lesões teciduais promovidas por outras bactérias septicemiantes em peixes, capazes de induzir lesões de natureza inflamatória, caracterizadas por injúrias sistêmicas (Lima et al, 2008). As doenças infecciosas são observadas com maior frequência em peixes ornamentais do que em peixes selvagens, pois provavelmente na natureza a debilidade ocasionada pela condição mórbida facilita a predação sumária e o peixe é eliminado do sistema, dificultando o seu registro (Thatcher, 1981).…”

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Isolamento de Alcaligenes faecalis em peixe Betta splendens Regan, 1910 no Brasil

et al. 2014

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This paper aimed to describe one case of infection byAlcaligenes faecalis in an ornamental fish Betta splendens, including clinical, pathological, and bacteriological evaluation. A female fish weighing 16 g initially showed discrete ulcerative lesions in the integument, inappetence, progressive weight loss, lethargy, dyspnea, and increased volume in the left lateral region. Necropsy revealed bilateral exophthalmia, corneal opacity, ascites, and subcutaneous abscess causing distension of the celomatic cavity. Histological changes of the skin were characterized by abscess, necrosis, hemorrhage, and inflammatory infiltrate consisting mainly of heterophils. Microbiological assays using the exudates and venous blood isolated exclusively A. faecalis.

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Isolation and characterizaton of Edwardsiella tarda from pacu Myleus micans

Cited by 20 publications

References 2 publications

Invasin of Edwardsiella tarda is essential for its haemolytic activity, biofilm formation and virulence towards fish

Invasin of Edwardsiella tarda is essential for its haemolytic activity, biofilm formation and virulence towards fish

The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.

Isolamento de Alcaligenes faecalis em peixe Betta splendens Regan, 1910 no Brasil

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