Rapid detection of Salmonella sp. in pork samples using fluorescent in situ hybridization: a comparison with VIDAS®-SLM system and ISO 6579 cultural method

Vieira-Pinto, Madalena; Oliveira, Manuela; Bernardo, Fernando; Martins, C.

doi:10.1590/s0102-09352007000600006

Cited by 21 publications

(7 citation statements)

References 24 publications

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“…infection in wild boars. Among the techniques, PCR has been claimed to be the most sensitive in detecting salmonellae in swine samples (Sanno et al, 2014;Vieira-Pinto, Oliveira, Bernardo, & Martins, 2007;Wacheck et al, 2010), although one article reports better results for culture methods in experimentally infected samples (Eriksson & Aspan, 2007). Better results of PCR-based methods could rely on the existence of certain serotypes difficult to culture, but also on the possibility of false positives due to non-viable bacteria (Eriksson & Aspan, 2007).…”

Section: Discussionmentioning

confidence: 99%

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Molino

Sánchez²,

Risco³

et al. 2019

Transbound Emerg Dis

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Summary The importance of wild boars as game species in Spain is well known. Their feeding habits and intrusive behaviour, together with a progressively wider spreading of populations, increases the interactions of these animals with livestock and humans. Considering that wild boars could have a potential role in the transmission of certain pathogens as salmonellae, the aims of this study were to determine the prevalence of Salmonella spp. in wild boars hunted in central‐western Spain, the occurrence of this pathogen in tonsils, mandibular lymph nodes and faeces (as markers for transmission risk), and to define the phylogenetic relationships among isolated strains, in order to investigate the circulation pathways of bacteria among tissues, animals and estates. Samples from 1,041 hunted wild boars were analysed for the presence of Salmonella spp. by bacteriological culture. Isolates were confirmed by PCR and serotyped in the Spanish national reference laboratory. The genetic relationships between strains were determined by PFGE. The results showed a 7.7% of positive animals (81 wild boars), being tonsils the organ most frequently colonised by Salmonella spp. (18.7%), followed by lymph nodes (5.1%) and faecal samples (2.9%). Serovars Enteritidis and Newport were the most frequent amongst the 34 different serovars obtained. The pulsed‐field gel electrophoresis (PGFE) analysis showed a great genetic diversity, with serovars that exhibited different pulsotypes when isolated from different estates and multiple serovars in the same estate. In conclusion, this study reveals the importance of wild boars as carriers and possible transmitters of virulent and/or antimicrobial‐resistant clones of Salmonella spp. to livestock and humans.

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Section: Discussionmentioning

confidence: 99%

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Molino

Sánchez²,

Risco³

et al. 2019

Transbound Emerg Dis

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“…This is most often attributed to the consumption of contaminated foods such as poultry, beef, pork, eggs, milk, seafood, nut products, and fresh produce (Foley and Lynne 2008). Recently, pork has been implicated as a major source of Salmonella contamination causing human salmonellosis (Vieira‐Pinto and others 2007). Among the 2500 serovars causing disease, Salmonella Typhimurium is reported to be the most common serotype associated with swine.…”

Section: Introductionmentioning

confidence: 99%

Loop‐Mediated Isothermal Amplification (LAMP) for the Rapid and Sensitive Detection of Salmonella Typhimurium from Pork

Techathuvanan

Draughon

D’Souza

2010

Journal of Food Science

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The novel loop-mediated isothermal amplification (LAMP) assay is a rapid, specific, and sensitive method that has potential application for routine diagnostics of Salmonella from pork products. The isothermal method does not require expensive equipment such as a PCR thermocycler but only a simple waterbath for amplification within 90 min. Detection is even simpler by visual eye or turbidimeters that are less expensive than fluorescent spectrophotometers or real-time PCR machines. All these advantages make it a practical approach for routine use by processing industries to rapidly detect Salmonella in their environment and to implement appropriate control strategies. To improve detection sensitivities, preenrichment followed by selective enrichment may be necessary. Even so, the entire assay can be completed at the most within two 8-h working shifts.

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“…Ribosomes of quickly-growing cells include a certain number of rRNA copies, usually over 1,000, which is sufficient for inducing a light signal after the labelled probe binds (Bottari et al, 2006). After the death of the cell, rRNA disintegrates, and the rate of this process depends, among others, on the concentration of enzymes -RNAz, as well as on the continuity of the cytoplasmic membrane (Vieira-Pinto et al, 2007). Slowly growing or metabolically inactive cells, containing a small number of ribosome copies, emit light of low intensity.…”

Section: Fish (Fluorescent In Situ Hybridization)mentioning

confidence: 99%

Detection of Salmonella spp. Presence in Food

Zadernowska¹,

Chajęcka²

2012

Salmonella - A Dangerous Foodborne Pathogen

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Rapid detection of Salmonella sp. in pork samples using fluorescent in situ hybridization: a comparison with VIDAS®-SLM system and ISO 6579 cultural method

Cited by 21 publications

References 24 publications

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Loop‐Mediated Isothermal Amplification (LAMP) for the Rapid and Sensitive Detection of Salmonella Typhimurium from Pork

Detection of Salmonella spp. Presence in Food

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