Prevalence of virulence factors in Escherichia coli strains isolated from the genital tract of healthy cows

Resende, Deisy Vivian de; Santo, Edilene; Macedo, Cristiana Santos de; Marin, José Moacir

doi:10.1590/s0102-09352007000300003

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…Bacteriological examination of uteruses of buffalo with endometritis revealed the presence of Escherichia coli . Most studies suggested that E. coli is considered the most important pathogen in the genital tract of bovine [25], [27], [32], [37]. Examination of uteruses of buffalo with endometritis also revealed the presence of Staphylococcus species such as epidermidis and aureus, Streptococcus pyogenes , Proteus vulgaris as well as Micrococcus , Klebsiella pneumonia and Clostridium perfringens .…”

Section: Discussionmentioning

confidence: 99%

Characterization of buffalo interleukin 8 (IL-8) and its expression in endometritis

Mossallam

Nahas

Mahfouz

et al. 2015

Journal of Genetic Engineering and Biotechnology

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River buffalo (Bubalus bubalis bubalis) with a population over 135 million heads is an important livestock. Interleukin 8 (IL-8) is a member of the chemokine family and is an important chemoattractant for neutrophils associated with a wide variety of inflammatory diseases such as endometritis. Tissue samples from the mammary gland, uterus and ovary were obtained from river buffalo (Mediterranean type) with and without endometritis. Bacteriological examination showed the presence of both gram positive and negative in all buffalo with endometritis. RNA extraction and complementary DNA (cDNA) synthesis were conducted from all tissues. Specific primer for IL8 full coding regions was designed using known cDNA sequences of Bubalus bubalis, Genbank accession number AY952930.1. IL-8 gene expression was investigated in buffalo tissues. Expression of IL-8 in buffalo with endometritis was found to increase significantly over buffalo without endometritis only in the uterus (P = 0.0159). PCR products from uterus tissues (target organs) of buffalo with and without endometritis, were purified and sequenced. No polymorphic sites were detected in the investigated samples. IL-8 cDNA nucleotide sequences of buffalo with and without endometritis were 100% identical (accession number JX413057). Buffalo IL8 cDNAs were compared with corresponding sequences of member of subfamily Bovinae (buffalo and cattle) and subfamily Caprinae (sheep and goat). IL-8 species specific differences were identified.

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Section: Discussionmentioning

confidence: 99%

Characterization of buffalo interleukin 8 (IL-8) and its expression in endometritis

Mossallam

Nahas

Mahfouz

et al. 2015

Journal of Genetic Engineering and Biotechnology

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“…Gen pap, gen sfa, dan gen afa merupakan faktor virulensi penting yang berhubungan dengan adhesin sebagai awal dari proses infeksi (Vincent et al, 2010;Okura dan Marin, 2014). Keberadaan gen yang bertanggung jawab terhadap adhesi (pap, sfa, dan afa) dapat dideteksi dengan menggunakan primer spesifik menggunakan metode polymerase chain reaction (PCR) (Resende et al, 2007).…”

Section: Pendahuluanunclassified

DETEKSI GEN PENYANDI ADHESIN PADA VEROCYTOTOXIGENIC Escherichia coli (VTEC) ISOLAT SAPI (Detection of Gene Encoding Adhesin of Verocytotoxigenic Escherichia coli (VTEC) Isolated from Cattles)

Prihtiyantoro¹,

Hartatik²,

Slipranata

et al. 2016

J Kedokt Hewan

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This study was aimed to perform phenotypic and genothypic characterization of Escherichia coli (E. coli), particularly VTEC strain isolated from cattle faeces. In this study, 25 E.coli isolated from faeces specimens and faeces base fertilizer of dairy and beef cattles were used. Examination were carried out using phenotypic and genothypic characterization which is specified for E coli VTEC strain. The result showed that 20 % samples of fresh faeces specimens were detected as VTEC strains and none of isolate was detected from faeces base fertilizer samples. From VTEC strains, could detect 16 % VT1 gene, 12 % VT2 genes and 8% of both. Detection on gene pyelonephritis-associated pilli (pap), S fimbrial adhesion (sfa), and afimbrial adhesion (afa) were found about 60%, 80% and 80%, respectively.

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Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows

et al. 2019

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Escherichia coli is the most common contaminant of the bovine uterus in the first few weeks postpartum, and one of the most important pathogens involved in uterine infections. This bacterium is characterised by diverse virulence factors (VF); however, the profile of E. coli VF in physiologic postpartum uterine contamination is not well established. Therefore, the present study aimed to investigate the prevalence of intrauterine bacteria, a set of virulence factors and phylogroups of E. coli isolates, leukogram and uterine cytology in 75 Holstein cows at 24 h (Sampling time 1) and 14 days (Sampling time 2) postpartum. Escherichia coli, α-haemolytic Streptococcus, Trueperella pyogenes, and Pasteurella multocida were the most common microorganisms isolated in pure culture, whereas E. coli and Clostridium perfringens Type A, E. coli and α-haemolytic Streptococcus, and E. coli and Proteus mirabilis were the most frequent microorganisms in mixed colonies at both studied sampling times. In the 59 E. coli isolates after 24 h (n = 35) and 14 days (n = 24) postpartum, the genes detected for VF were fimH, iucD/aer, kps, hlyA, usp, vt1 and vt2. Most E. coli strains detected in both moments of study belonged to Commensal phylogenetic groups A and B1, whereas Pathogenic groups D and B2 were identified at 24 h and 14 days postpartum respectively. All cows presented suitable immune response against the presence of bacteria in uterine lumen, observed by leukocytosis, neutrophilia, lymphocytosis and monocytosis at leukogram and a high number of polymorphonuclear leukocytes in uterine cytology, in both studied moments. Thus, a complex diversity of aerobic and anaerobic bacteria is involved in uterine contamination in the early postpartum of cows, besides the predominance of E. coli. Moreover, the genes fimH, iucD/aer, hlyA, kps, usp, vt1 and vt2 play a key role in the virulence of E. coli in this period.

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Prevalence of virulence factors in Escherichia coli strains isolated from the genital tract of healthy cows

Cited by 3 publications

References 16 publications

Characterization of buffalo interleukin 8 (IL-8) and its expression in endometritis

Characterization of buffalo interleukin 8 (IL-8) and its expression in endometritis

DETEKSI GEN PENYANDI ADHESIN PADA VEROCYTOTOXIGENIC Escherichia coli (VTEC) ISOLAT SAPI (Detection of Gene Encoding Adhesin of Verocytotoxigenic Escherichia coli (VTEC) Isolated from Cattles)

Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows

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