Antibodies against pseudorabies virus in feral swine in southeast Brazil

Abstract

Serum samples collected from 358 wild boars (Sus scrofa) in breeding farms in São Paulo, southeast Brazil, from 1998 to 2001, were tested for antibodies against pseudorabies virus (PRV) by means of serum neutralization (SN) and enzyme-linked immunobsorbent assay (ELISA). Seropositive animals were detected in three of seven herds analyzed. Overall seroprevalence as assessed by SN was 30.7%, ranging from 25.2% to 100% for the herds that presented seropositive animals. Indirect ELISA detected lower seroprevalence (19.3%). Sensitivity and specificity of ELISA were equal to 57.3% and 97.6%, respectively. Agreement was equal to 85.2% (P<0.0001). These results showed that PRV infections occurred in farmed feral swine in southeast Brazil, and affect pseudorabies eradication program.

Serosurvey for Pseudorabies (Aujeszky's Disease) in Free-Range Wild Boars (Sus scrofa) of Brazil

“…There has not been a previous report of PRV in free-ranging wild boars in Brazil. The seroprevalence to PRV that we report was lower than that reported in Brazilian farmed wild boars, 19.3% (69/358) seropositive animals (Cunha et al 2006). In addition, although restricted to the Brazilian Pantanal biome (floodplains), feral pigs, classified as Sus scrofa have a higher seroprevalence to PRV, with 47.3% (88 of 186) positive animals (Paes et al 2013).…”

“…The presence of antibodies against PRV was performed at the Biological Institute, a National Livestock Reference Laboratory (São Paulo, Brazil) with wild boar serum samples were tested in duplicate by a commercially available enzyme-linked immunosorbent assay (IDEXX PRV/ADV gB Ab Testt, IDEXX Laboratories, Westbrook, Maine, USA) and results interpreted as positive or negative. Enzyme-linked immunosorbent assay sensitivity has been considered greater than serum neutralization and has been recommended as the international gold standard serological method for PRV diagnosis and notification by the World Organization for Animal Health (Cunha et al 2006; World Organization for Animal Health 2018).…”

Different methods of real-time PCR for detection of pseudorabies virus

“…Two researches were done in Brazil. The first one used 358 samples from 1998-2001 collected from different farms and demonstrated prevalence varying from 0% to 55.9% positive samples in ELISA (CUNHA et al, 2006). The second research was done with 186 samples collected from feral pigs throughout the Brazilian wetland plain, obtaining 31.8% positive in serum neutralization (PAES et al, 2013).…”

Estratégia para erradicação de focos da Doença de Aujeszky em suínos no Estado de São Paulo

“…Pesquisas demonstram que no estado de São Paulo há circulação do vírus responsável pela DA (Secretaria de Agricultura e Abastecimento 1986, Cunha et al 2006, Villalobos et al 2008.…”