Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis

Coutinho, Selene Dall’Acqua

doi:10.1590/s0102-09352005000800003

Cited by 7 publications

(8 citation statements)

References 16 publications

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“…However, the fact the seborrhea was the most frequent dermatopathy found in the diagnosis (10) must be taken into consideration. The yeast pathogen has been discussed in several studies which have described M. pachydermatis as a pathogenous opportunist which thrives in the medium where there is an increase in humidity and lipid cerumen composition, among other favorable factors (Amaral et al, 1998;Coutinho, 2005). Cocker Spaniel English, Daschund and Poodle breeds showing dermatopathies, as well as animals of no definite breed, were the ones which presented the most dermatological problems, followed by Sharpei, Labrador, German Shepherd, Boxer and Bulldog breeds, though their sampling was not significant due to the few individuals available for each breed.…”

Section: Discussionmentioning

confidence: 99%

Malassezia pachydermatis presence in canine external otitis and dermatopathies

Nascente¹,

Meinerz²,

Faria³

et al. 2015

J. Yeast Fungal Res.

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Malassezia pachydermatis is a commensal yeast of the skin and external ear canal of dogs and cats that may become an opportunistic pathogen under some conditions. The aim of this paper was to research the diseases which predispose to the development of opportunistic M. pachydermatis in dogs with external otitis or dermatopathies. The samples were collected by sterile swabs or by the carpet technique. Samples were obtained from the external ear canal and cutaneous tegument and were seeded in Sabouraud dextrose agar added with chloramphenicol and cycloheximide. Following, they were incubated at 32ºC for seven days for yeast identification. M. pachydermatis isolation was successful in 49 (70%) of the 70 samples investigated. Underlying diseases diagnosed in these animals included ceruminous otitis, skin fold pyoderma, seborrhoeic dermatitis, atopic dermatitis, pressure point pyoderma, parasitic otitis, acanthosis nigricans, hot spot and bacterial otitis. The results confirm that the presence of this yeast can cause the perpetuation of these diseases and be a treatment complication factor.

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Section: Discussionmentioning

confidence: 99%

Malassezia pachydermatis presence in canine external otitis and dermatopathies

Nascente¹,

Meinerz²,

Faria³

et al. 2015

J. Yeast Fungal Res.

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“…Para la evaluación de la actividad fosfolipasa, se utilizó agar Sabouraud con 10% (v/v) de yema de huevo, el medio fue inoculado de forma directa en cuatro puntos de la caja a partir de cultivos frescos e incubados a 32°C durante 21 días. Para determinar la actividad fosfolipasa se calculó el índice Pz, que equivale al valor resultante de la relación del tamaño de la colonia y el tamaño de la colonia más el halo de hidrólisis que se genera alrededor de la misma (15,16,24). Fue considerada actividad fosfolipasa nula cuando Pz=1, actividad fosfolipasa alta con valores Pz entre 0.64-0.99 y actividad fosfolipasa muy alta con Pz inferiores a 0.64 (15,24), la lectura se realizó al día 21 promediando los valores Pz de los cuatro puntos de siembra.…”

Section: Microorganismsunclassified

“…The medium was inoculated from fresh cultures directly at four different points in the Petri dish, and incubated at 32°C for 21 days. To determine phospholipase activity the ratio of colony diameter to diameter of the dense white zone of precipitation around phospholipase positive colonies (Pz value) was used (15,16,24). No phospholipase activity was considered for a Pz value of 1 (Pz=1).…”

Section: Biochemical and Physiological Characterizationmentioning

confidence: 99%

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Caracterización fenotípica de aislamientos de Malassezia spp., de origen canino

et al. 2016

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Objective. To characterize and identify yeasts of the genus Malassezia by phenotypic features. Materials and methods. First, the macroscopic and microscopic morphological characteristics were described. In addition we performed biochemical and physiological assays as Tweens and Cremophor, including more. Results. Our results evidenced of 105 isolates obtained from dogs diagnosed with external otitis, it was possible to identify two distinct species from 46 isolates within the Malassezia genus: 36.19% (n=38) were identified as M. pachydermatis and 7.62% (n=8) as M. furfur. According to phenotypic patterns the remaining 56.19% (n=59) were reported as Malassezia spp., possibly corresponding to M. furfur and/or M. pachydermatis. Conclusions. Results emphasize the necessity to characterize according to species. It is not feasible to define Malassezia by species based on morphological, biochemical, and physiological findings. Therefore, molecular genotyping should be performed to identify markers allowing a more precise isolate identification. This would broaden our epidemiological knowledge regarding different species involved in canine otitis pathologies. Keywords: Biochemical techniques, external otitis, Malassezia, morphology. (Source: CAB) RESUMENObjetivo. Caracterizar e identificar levaduras del género Malassezia, mediante características fenotípicas. Materiales y métodos. Inicialmente se describieron las características morfológicas macroscópicas y microscópicas, adicionalmente se realizaron pruebas bioquímicas y fisiológicas como Tween y Cremophor, entre otras. Resultados. De 105 aislamientos de caninos diagnosticados previamente con otitis, 46 fueron caracterizados hasta especie, así: El 36.19% (n=38) correspondió a M. pachydermatis, el 7.2% (n=8) a M. furfur; y 56.19% (n=59) restante fueron reportados como Malassezia spp., debido a los patrones fenotípicos atípicos que presentaron, y que podrían corresponder a variantes de M. furfur y/o M. pachydermatis. Conclusión. Estos resultados enfatizan la necesidad de hacer una caracterización a nivel de especie y/o genotipos mediante marcadores moleculares que permitan una identificación más precisa de los aislamientos. Con el presente estudio, se contribuye al conocimiento de las diferentes especies involucradas en patologías óticas en caninos.

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“…Based on the observation of the genus Candida species has been shown that samples of M. pachydermatis isolated from the conduct of hearing dogs with otitis are also able to produce these enzymes (8). The proteolytic enzymes are part of the metabolic system of a large number of living organisms, can that be isolated from animal, vegetable or microorganisms and it is are classified as proteases and peptidase.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and identification of yeasts in dogs external ear canal with and without otitis

Bona

Telesca

2012

Rev MVZ Córdoba

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Objective. To analyze the presence of yeast in the external ear canal of 116 dogs with and without a diagnosis of otitis from veterinary clinic in the Chapecó city, Santa Catarina, Brazil, and to examine the secretion of the proteinase in isolates. Materials and methods. Were collected cerumen of conduct hearing of dogs of 16 different races 71% with pendular ear type, 5% of semi-pendular and 24% of the erect type. All dogs were previously evaluated by otoscopy and grouped in dogs with and without otitis. Results. Yeasts were isolated in 44 samples (approximately 36%), where Malassezia pachydermatis was identified in 95% of samples where were observed growth of yeasts. On 20 samples the proteinase enzyme showed strong activity in 31% isolates, were 21% of the dogs with otitis tested showed high proteolytic activity. Conclusions. We observed a variation of strains of M. pachydermatis-producing enzymes. The variation in production of these enzymes is probably more associated with different response to the action of the immune system of the animal in the tissue injury.Key words: Dog, Malassezia pachydermatis, otitis, proteinases (Fuente: CAB). RESUMENObjetivo. Se investigó la presencia de levaduras en el canal externo del oído de 116 perros de la clínica veterinaria en la ciudad de Chapecó, Santa Catarina,Brasil, en perros sanos y perros con otitis y se examinó la secreción de la proteinasa en las muestra aisladas. Materiales y métodos. Se recogieron cerumen del oído de perros de 16 razas diferentes, dónde 71% fue de oído de tipo pendular, 5% de semi-pendular y 24% del tipo erecto. Todos los perros fueron evaluados previamente por otoscopia y agrupados en perros con y sin otitis externa. Resultados. Las levaduras se aislaron en 44 muestras (aproximadamente 36%), donde Malassezia pachydermatis se identificó en el 95% de las muestras donde se observó el crecimiento de las levaduras. El 20 muestras la secreción de proteinasa mostró fuerte actividad en el 31% de los aislados y en 21% de los perros con otitis mostró alta actividad proteolítica. Conclusiones. Hemos observado una variación de cepas de M. pachydermatis productoras de enzimas. La variación en la producción de estas enzimas es probablemente más asociados con la respuesta diferente a la acción del sistema inmunológico del animal en la lesión tisular.Palabras clave: Malassezia pachydermatis, otitis, perro, proteinasa (Source: CAB). COMUNICACIÓN BREVERev.MVZ Córdoba 17(2):3059-3064, 2012. 3060REVISTA MVZ CÓRDOBA • Volumen 17(2), Mayo -Agosto 2012

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Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis

Cited by 7 publications

References 16 publications

Malassezia pachydermatis presence in canine external otitis and dermatopathies

Malassezia pachydermatis presence in canine external otitis and dermatopathies

Caracterización fenotípica de aislamientos de Malassezia spp., de origen canino

Occurrence and identification of yeasts in dogs external ear canal with and without otitis

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